Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis

Abstract Endometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera...

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Autores principales:	Katarzyna Sołkiewicz, Hubert Krotkiewski, Marcin Jędryka, Ewa M. Kratz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3b670821f854b73a3725189a504737b
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spelling	oai:doaj.org-article:f3b670821f854b73a3725189a504737b2021-12-02T15:52:58ZVariability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis10.1038/s41598-021-85200-x2045-2322https://doaj.org/article/f3b670821f854b73a3725189a504737b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85200-xhttps://doaj.org/toc/2045-2322Abstract Endometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera of women classified as: with endometriosis, without endometriosis but with some benign ginecological disease, and control group of healthy women, in context of its utility for differentiation of advanced endometriosis from the group of healthy women. IgG sialylation and galactosylation/agalactosylation degree was determined using specific lectins: MAA and SNA detecting sialic acid α2,3- and α2,6-linked, respectively, RCA-I and GSL-II specific to terminal Gal and terminal GlcNAc, respectively. The results of ROC and cluster analysis showed that the serum IgG MAA-reactivity, sialylation and agalactosylation factor may be used as supplementary parameters for endometriosis diagnostics and could be taken into account as a useful clinical tool to elucidate women with high risk of endometriosis development. Additionally, we have shown that the analysis of native serum IgG glycosylation, without the prior time-consuming and expensive isolation of the protein, is sufficient to differentiation endometriosis from a group of healthy women.Katarzyna SołkiewiczHubert KrotkiewskiMarcin JędrykaEwa M. KratzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Sołkiewicz Hubert Krotkiewski Marcin Jędryka Ewa M. Kratz Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
description	Abstract Endometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera of women classified as: with endometriosis, without endometriosis but with some benign ginecological disease, and control group of healthy women, in context of its utility for differentiation of advanced endometriosis from the group of healthy women. IgG sialylation and galactosylation/agalactosylation degree was determined using specific lectins: MAA and SNA detecting sialic acid α2,3- and α2,6-linked, respectively, RCA-I and GSL-II specific to terminal Gal and terminal GlcNAc, respectively. The results of ROC and cluster analysis showed that the serum IgG MAA-reactivity, sialylation and agalactosylation factor may be used as supplementary parameters for endometriosis diagnostics and could be taken into account as a useful clinical tool to elucidate women with high risk of endometriosis development. Additionally, we have shown that the analysis of native serum IgG glycosylation, without the prior time-consuming and expensive isolation of the protein, is sufficient to differentiation endometriosis from a group of healthy women.
format	article
author	Katarzyna Sołkiewicz Hubert Krotkiewski Marcin Jędryka Ewa M. Kratz
author_facet	Katarzyna Sołkiewicz Hubert Krotkiewski Marcin Jędryka Ewa M. Kratz
author_sort	Katarzyna Sołkiewicz
title	Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
title_short	Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
title_full	Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
title_fullStr	Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
title_full_unstemmed	Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis
title_sort	variability of serum igg sialylation and galactosylation degree in women with advanced endometriosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3b670821f854b73a3725189a504737b
work_keys_str_mv	AT katarzynasołkiewicz variabilityofserumiggsialylationandgalactosylationdegreeinwomenwithadvancedendometriosis AT hubertkrotkiewski variabilityofserumiggsialylationandgalactosylationdegreeinwomenwithadvancedendometriosis AT marcinjedryka variabilityofserumiggsialylationandgalactosylationdegreeinwomenwithadvancedendometriosis AT ewamkratz variabilityofserumiggsialylationandgalactosylationdegreeinwomenwithadvancedendometriosis
_version_	1718385547458641920

Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis

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