Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny

Abstract Helminths are large multicellular parasites that infect one quarter of the human population. To prolong their survival, helminths suppress the immune responses of their hosts. Strongyloides ratti delays its expulsion from the gut by induction of regulatory circuits in a mouse strain-specifi...

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Autores principales:	Wiebke Hartmann, Birte Blankenhaus, Marie-Luise Brunn, Jana Meiners, Minka Breloer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3c26d072329439ea2776bc719c1441d
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spelling	oai:doaj.org-article:f3c26d072329439ea2776bc719c1441d2021-12-02T14:12:08ZElucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny10.1038/s41598-020-79477-72045-2322https://doaj.org/article/f3c26d072329439ea2776bc719c1441d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79477-7https://doaj.org/toc/2045-2322Abstract Helminths are large multicellular parasites that infect one quarter of the human population. To prolong their survival, helminths suppress the immune responses of their hosts. Strongyloides ratti delays its expulsion from the gut by induction of regulatory circuits in a mouse strain-specific manner: depletion of Foxp3+ regulatory T cells (Treg) improves the anti-S. ratti immunity in BALB/c but not in C57BL/6 mice. In the current study we compare the hierarchy of immunoregulatory pathways in BALB/c, C57BL/6 mice and their F1 progeny (BALB/c × C57BL/6). Using multicolor flow cytometry, we show that S. ratti induces a distinct pattern of inhibitory checkpoint receptors by Foxp3+ Treg and Foxp3− T cells. Intensity of expression was highest in C57BL/6 and lowest in BALB/c mice, while the F1 cross had an intermediate phenotype or resembled BALB/c mice. Treg subsets expanded during infection in all three mouse strains. Similar to BALB/c mice, depletion of Treg reduced intestinal parasite burden and increased mucosal mast cell activation in S. ratti-infected F1 mice. Our data indicate that Treg dominate the regulation of immune responses in BALB/c and F1 mice, while multiple regulatory layers exist in C57BL/6 mice that may compensate for the absence of Treg.Wiebke HartmannBirte BlankenhausMarie-Luise BrunnJana MeinersMinka BreloerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wiebke Hartmann Birte Blankenhaus Marie-Luise Brunn Jana Meiners Minka Breloer Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
description	Abstract Helminths are large multicellular parasites that infect one quarter of the human population. To prolong their survival, helminths suppress the immune responses of their hosts. Strongyloides ratti delays its expulsion from the gut by induction of regulatory circuits in a mouse strain-specific manner: depletion of Foxp3+ regulatory T cells (Treg) improves the anti-S. ratti immunity in BALB/c but not in C57BL/6 mice. In the current study we compare the hierarchy of immunoregulatory pathways in BALB/c, C57BL/6 mice and their F1 progeny (BALB/c × C57BL/6). Using multicolor flow cytometry, we show that S. ratti induces a distinct pattern of inhibitory checkpoint receptors by Foxp3+ Treg and Foxp3− T cells. Intensity of expression was highest in C57BL/6 and lowest in BALB/c mice, while the F1 cross had an intermediate phenotype or resembled BALB/c mice. Treg subsets expanded during infection in all three mouse strains. Similar to BALB/c mice, depletion of Treg reduced intestinal parasite burden and increased mucosal mast cell activation in S. ratti-infected F1 mice. Our data indicate that Treg dominate the regulation of immune responses in BALB/c and F1 mice, while multiple regulatory layers exist in C57BL/6 mice that may compensate for the absence of Treg.
format	article
author	Wiebke Hartmann Birte Blankenhaus Marie-Luise Brunn Jana Meiners Minka Breloer
author_facet	Wiebke Hartmann Birte Blankenhaus Marie-Luise Brunn Jana Meiners Minka Breloer
author_sort	Wiebke Hartmann
title	Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
title_short	Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
title_full	Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
title_fullStr	Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
title_full_unstemmed	Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny
title_sort	elucidating different pattern of immunoregulation in balb/c and c57bl/6 mice and their f1 progeny
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3c26d072329439ea2776bc719c1441d
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Elucidating different pattern of immunoregulation in BALB/c and C57BL/6 mice and their F1 progeny

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