A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for oth...

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Autores principales: Pascal Püllmann, Anja Knorrscheidt, Judith Münch, Paul R. Palme, Wolfgang Hoehenwarter, Sylvestre Marillonnet, Miguel Alcalde, Bernhard Westermann, Martin J. Weissenborn
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/f3c63e6d684b4ea8b0fbe616270d80cf
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spelling oai:doaj.org-article:f3c63e6d684b4ea8b0fbe616270d80cf2021-12-02T15:43:08ZA modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases10.1038/s42003-021-02076-32399-3642https://doaj.org/article/f3c63e6d684b4ea8b0fbe616270d80cf2021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02076-3https://doaj.org/toc/2399-3642Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for other genes of interest that are suitable for production in yeast.Pascal PüllmannAnja KnorrscheidtJudith MünchPaul R. PalmeWolfgang HoehenwarterSylvestre MarillonnetMiguel AlcaldeBernhard WestermannMartin J. WeissenbornNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-20 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Pascal Püllmann
Anja Knorrscheidt
Judith Münch
Paul R. Palme
Wolfgang Hoehenwarter
Sylvestre Marillonnet
Miguel Alcalde
Bernhard Westermann
Martin J. Weissenborn
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
description Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for other genes of interest that are suitable for production in yeast.
format article
author Pascal Püllmann
Anja Knorrscheidt
Judith Münch
Paul R. Palme
Wolfgang Hoehenwarter
Sylvestre Marillonnet
Miguel Alcalde
Bernhard Westermann
Martin J. Weissenborn
author_facet Pascal Püllmann
Anja Knorrscheidt
Judith Münch
Paul R. Palme
Wolfgang Hoehenwarter
Sylvestre Marillonnet
Miguel Alcalde
Bernhard Westermann
Martin J. Weissenborn
author_sort Pascal Püllmann
title A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
title_short A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
title_full A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
title_fullStr A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
title_full_unstemmed A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
title_sort modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/f3c63e6d684b4ea8b0fbe616270d80cf
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