High diversity droplet microfluidic libraries generated with a commercial liquid spotter

Abstract Droplet libraries consisting of many reagents encapsulated in separate droplets are necessary for applications of microfluidics, including combinatorial chemical synthesis, DNA-encoded libraries, and massively multiplexed PCR. However, existing approaches for generating them are laborious a...

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Autores principales:	Jesse Q. Zhang, Christian A. Siltanen, Ata Dolatmoradi, Chen Sun, Kai-Chun Chang, Russell H. Cole, Zev J. Gartner, Adam R. Abate
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3cff36afa924cc0a4bb916acd1bb7cd
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spelling	oai:doaj.org-article:f3cff36afa924cc0a4bb916acd1bb7cd2021-12-02T16:23:14ZHigh diversity droplet microfluidic libraries generated with a commercial liquid spotter10.1038/s41598-021-83865-y2045-2322https://doaj.org/article/f3cff36afa924cc0a4bb916acd1bb7cd2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83865-yhttps://doaj.org/toc/2045-2322Abstract Droplet libraries consisting of many reagents encapsulated in separate droplets are necessary for applications of microfluidics, including combinatorial chemical synthesis, DNA-encoded libraries, and massively multiplexed PCR. However, existing approaches for generating them are laborious and impractical. Here, we describe an automated approach using a commercial array spotter. The approach can controllably emulsify hundreds of different reagents in a fraction of the time of manual operation of a microfluidic device, and without any user intervention. We demonstrate that the droplets produced by the spotter are similarly uniform to those produced by microfluidics and automate the generation of a ~ 2 mL emulsion containing 192 different reagents in ~ 4 h. The ease with which it can generate high diversity droplet libraries should make combinatorial applications more feasible in droplet microfluidics. Moreover, the instrument serves as an automated droplet generator, allowing execution of droplet reactions without microfluidic expertise.Jesse Q. ZhangChristian A. SiltanenAta DolatmoradiChen SunKai-Chun ChangRussell H. ColeZev J. GartnerAdam R. AbateNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jesse Q. Zhang Christian A. Siltanen Ata Dolatmoradi Chen Sun Kai-Chun Chang Russell H. Cole Zev J. Gartner Adam R. Abate High diversity droplet microfluidic libraries generated with a commercial liquid spotter
description	Abstract Droplet libraries consisting of many reagents encapsulated in separate droplets are necessary for applications of microfluidics, including combinatorial chemical synthesis, DNA-encoded libraries, and massively multiplexed PCR. However, existing approaches for generating them are laborious and impractical. Here, we describe an automated approach using a commercial array spotter. The approach can controllably emulsify hundreds of different reagents in a fraction of the time of manual operation of a microfluidic device, and without any user intervention. We demonstrate that the droplets produced by the spotter are similarly uniform to those produced by microfluidics and automate the generation of a ~ 2 mL emulsion containing 192 different reagents in ~ 4 h. The ease with which it can generate high diversity droplet libraries should make combinatorial applications more feasible in droplet microfluidics. Moreover, the instrument serves as an automated droplet generator, allowing execution of droplet reactions without microfluidic expertise.
format	article
author	Jesse Q. Zhang Christian A. Siltanen Ata Dolatmoradi Chen Sun Kai-Chun Chang Russell H. Cole Zev J. Gartner Adam R. Abate
author_facet	Jesse Q. Zhang Christian A. Siltanen Ata Dolatmoradi Chen Sun Kai-Chun Chang Russell H. Cole Zev J. Gartner Adam R. Abate
author_sort	Jesse Q. Zhang
title	High diversity droplet microfluidic libraries generated with a commercial liquid spotter
title_short	High diversity droplet microfluidic libraries generated with a commercial liquid spotter
title_full	High diversity droplet microfluidic libraries generated with a commercial liquid spotter
title_fullStr	High diversity droplet microfluidic libraries generated with a commercial liquid spotter
title_full_unstemmed	High diversity droplet microfluidic libraries generated with a commercial liquid spotter
title_sort	high diversity droplet microfluidic libraries generated with a commercial liquid spotter
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3cff36afa924cc0a4bb916acd1bb7cd
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High diversity droplet microfluidic libraries generated with a commercial liquid spotter

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