Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis
Background: Extracellular heat-shock proteins (eHsp) are highly conserved molecules that play an important role in inflammatory diseases and have been quantified in plasma from patients with infectious diseases, including sepsis. There is a constant search for dependable biochemical markers that, in...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:f3d4539086c7482d8e9a62d39684820d2021-12-01T02:14:34ZIncreased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis2296-236010.3389/fped.2021.740274https://doaj.org/article/f3d4539086c7482d8e9a62d39684820d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.740274/fullhttps://doaj.org/toc/2296-2360Background: Extracellular heat-shock proteins (eHsp) are highly conserved molecules that play an important role in inflammatory diseases and have been quantified in plasma from patients with infectious diseases, including sepsis. There is a constant search for dependable biochemical markers that, in combination with conventional methods, could deliver a prompt and reliable diagnosis of early-onset neonatal sepsis.Objective: We sought to assess the level of eHsp-27, eHsp-60, eHsp-70, and tumor necrosis factor-alpha (TNFα) in plasma of healthy neonates at term and infants with early-onset neonatal sepsis.Methods: This study included 34 newborns that were classified as healthy neonates at term (blood samples from the umbilical cord, n = 23) or infants with early-onset neonatal sepsis (blood samples obtained from umbilical artery by standard sterile procedures before starting a systemic antibiotic intervention, n = 11). All blood samples were centrifuged, and the plasma recovered to determine eHsp-27, eHsp-60, eHsp-70, and TNFα levels by ELISA.Results: Our results indicate that the level of eHsp-27 in healthy neonates at term was 0.045 ± 0.024 pg/ml. This value decreased 2.5-fold in infants with early-onset neonate sepsis (0.019 ± 0.006 pg/ml, p = 0.004). In contrast, the levels of eHsp-60 and eHsp-70 in healthy neonates at term were 13.69 ± 5.3 and 4.03 ± 2.6 pg/ml, respectively. These protein levels increased significantly 1.8- and 1.9-fold in the plasma of infants with early-onset neonatal sepsis (p ≤ 0.001). The level of TNFα in healthy neonates at term was 2.94 ± 0.46 pg/ml, with a 3.0-fold increase in infants with early-onset neonatal sepsis (8.96 ± 0.72 pm/ml, p ≤ 0.001). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of eHsp compared with that of C-reactive protein were 73.3, 60.0, 47.8, and 33.3%, respectively.Conclusion: This study demonstrated a consistent increase of eHsp-60 and eHsp-70 in the plasma of infants diagnosed with early-onset neonatal sepsis. These proteins showed higher sensitivity and specificity than C-reactive protein and blood culture test.Arturo Alejandro Canul-EuanGibran Zúñiga-GonzálezJanelly Estefania Palacios-LunaRolando Maida-ClarosNéstor Fabián DíazPatricia Saltigeral-TigeralPerla Karina García-MayOscar Díaz-RuizHéctor Flores-HerreraFrontiers Media S.A.articleearly-neonatal sepsisextracellular heat-shock proteinneonatal intensive care unittumor necrosis factor alphaneonatal infectionPediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021) |
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early-neonatal sepsis extracellular heat-shock protein neonatal intensive care unit tumor necrosis factor alpha neonatal infection Pediatrics RJ1-570 |
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early-neonatal sepsis extracellular heat-shock protein neonatal intensive care unit tumor necrosis factor alpha neonatal infection Pediatrics RJ1-570 Arturo Alejandro Canul-Euan Gibran Zúñiga-González Janelly Estefania Palacios-Luna Rolando Maida-Claros Néstor Fabián Díaz Patricia Saltigeral-Tigeral Perla Karina García-May Oscar Díaz-Ruiz Héctor Flores-Herrera Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| description |
Background: Extracellular heat-shock proteins (eHsp) are highly conserved molecules that play an important role in inflammatory diseases and have been quantified in plasma from patients with infectious diseases, including sepsis. There is a constant search for dependable biochemical markers that, in combination with conventional methods, could deliver a prompt and reliable diagnosis of early-onset neonatal sepsis.Objective: We sought to assess the level of eHsp-27, eHsp-60, eHsp-70, and tumor necrosis factor-alpha (TNFα) in plasma of healthy neonates at term and infants with early-onset neonatal sepsis.Methods: This study included 34 newborns that were classified as healthy neonates at term (blood samples from the umbilical cord, n = 23) or infants with early-onset neonatal sepsis (blood samples obtained from umbilical artery by standard sterile procedures before starting a systemic antibiotic intervention, n = 11). All blood samples were centrifuged, and the plasma recovered to determine eHsp-27, eHsp-60, eHsp-70, and TNFα levels by ELISA.Results: Our results indicate that the level of eHsp-27 in healthy neonates at term was 0.045 ± 0.024 pg/ml. This value decreased 2.5-fold in infants with early-onset neonate sepsis (0.019 ± 0.006 pg/ml, p = 0.004). In contrast, the levels of eHsp-60 and eHsp-70 in healthy neonates at term were 13.69 ± 5.3 and 4.03 ± 2.6 pg/ml, respectively. These protein levels increased significantly 1.8- and 1.9-fold in the plasma of infants with early-onset neonatal sepsis (p ≤ 0.001). The level of TNFα in healthy neonates at term was 2.94 ± 0.46 pg/ml, with a 3.0-fold increase in infants with early-onset neonatal sepsis (8.96 ± 0.72 pm/ml, p ≤ 0.001). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of eHsp compared with that of C-reactive protein were 73.3, 60.0, 47.8, and 33.3%, respectively.Conclusion: This study demonstrated a consistent increase of eHsp-60 and eHsp-70 in the plasma of infants diagnosed with early-onset neonatal sepsis. These proteins showed higher sensitivity and specificity than C-reactive protein and blood culture test. |
| format |
article |
| author |
Arturo Alejandro Canul-Euan Gibran Zúñiga-González Janelly Estefania Palacios-Luna Rolando Maida-Claros Néstor Fabián Díaz Patricia Saltigeral-Tigeral Perla Karina García-May Oscar Díaz-Ruiz Héctor Flores-Herrera |
| author_facet |
Arturo Alejandro Canul-Euan Gibran Zúñiga-González Janelly Estefania Palacios-Luna Rolando Maida-Claros Néstor Fabián Díaz Patricia Saltigeral-Tigeral Perla Karina García-May Oscar Díaz-Ruiz Héctor Flores-Herrera |
| author_sort |
Arturo Alejandro Canul-Euan |
| title |
Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| title_short |
Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| title_full |
Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| title_fullStr |
Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| title_full_unstemmed |
Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis |
| title_sort |
increased levels of plasma extracellular heat-shock proteins 60 and 70 kda characterized early-onset neonatal sepsis |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f3d4539086c7482d8e9a62d39684820d |
| work_keys_str_mv |
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