Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system

Abstract To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are...

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Autores principales:	Fan Yang, Gang Li, Jiamin Yang, Zhenhai Wang, Danhong Han, Fengjie Zheng, Shengyong Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3db0ff096e846019d23b4d6a0ea8370
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spelling	oai:doaj.org-article:f3db0ff096e846019d23b4d6a0ea83702021-12-02T11:41:21ZMeasurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system10.1038/s41598-017-01891-12045-2322https://doaj.org/article/f3db0ff096e846019d23b4d6a0ea83702017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01891-1https://doaj.org/toc/2045-2322Abstract To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute increments of local temperature in micro-Testing Zones induced by live cells. In this method, built-in high-performance micro-thermocouple arrays and double-stabilized system with a stability of 10 mK were applied. Increments of local temperature close to adherent human hepatoblastoma (HepG2) cells were continuously recorded for days without stimulus, showing frequent fluctuations within 60 mK and a maximum increment by 285 mK. This method may open a door for real-time recording of the absolute local temperature increments of individual cells, therefore offering valuable information for cell biology and clinical therapy in the field of cancer research.Fan YangGang LiJiamin YangZhenhai WangDanhong HanFengjie ZhengShengyong XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan Yang Gang Li Jiamin Yang Zhenhai Wang Danhong Han Fengjie Zheng Shengyong Xu Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
description	Abstract To monitor the temperature distribution of a cell and its changes under varied conditions is currently a technical challenge. A variety of non-contact methods used for measuring cellular temperature have been developed, where changes of local temperature at cell-level and sub-cell-level are indirectly calculated through the changes in intensity, band-shape, bandwidth, lifetime or polarization anisotropy of the fluorescence spectra recorded from the nano-sized fluorescent materials pre-injected into the target cell. Unfortunately, the optical properties of the fluorescent nano-materials may be affected by complicated intracellular environment, leading to unexpected measurement errors and controversial arguments. Here, we attempted to offer an alternative approach for measuring the absolute increments of local temperature in micro-Testing Zones induced by live cells. In this method, built-in high-performance micro-thermocouple arrays and double-stabilized system with a stability of 10 mK were applied. Increments of local temperature close to adherent human hepatoblastoma (HepG2) cells were continuously recorded for days without stimulus, showing frequent fluctuations within 60 mK and a maximum increment by 285 mK. This method may open a door for real-time recording of the absolute local temperature increments of individual cells, therefore offering valuable information for cell biology and clinical therapy in the field of cancer research.
format	article
author	Fan Yang Gang Li Jiamin Yang Zhenhai Wang Danhong Han Fengjie Zheng Shengyong Xu
author_facet	Fan Yang Gang Li Jiamin Yang Zhenhai Wang Danhong Han Fengjie Zheng Shengyong Xu
author_sort	Fan Yang
title	Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
title_short	Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
title_full	Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
title_fullStr	Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
title_full_unstemmed	Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system
title_sort	measurement of local temperature increments induced by cultured hepg2 cells with micro-thermocouples in a thermally stabilized system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f3db0ff096e846019d23b4d6a0ea8370
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Measurement of local temperature increments induced by cultured HepG2 cells with micro-thermocouples in a thermally stabilized system

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