Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models

Psoriasis (Ps), commonly perceived as a skin and joint disorder, has a complex basis and results from disturbances in the sophisticated network between skin and the immune system. This makes it difficult to properly depict the complete pathomechanism on an in vitro scale. Deciphering the complicated...

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Autores principales:	Katarzyna Bocheńska, Marta Moskot, Magdalena Gabig-Cimińska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell-based models in vitro cultures skin epidermal keratinocytes monocytes psoriasis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f3e77c1011044c06b84d77aa25a2fccd
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spelling	oai:doaj.org-article:f3e77c1011044c06b84d77aa25a2fccd2021-11-25T17:10:04ZUse of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models10.3390/cells101129852073-4409https://doaj.org/article/f3e77c1011044c06b84d77aa25a2fccd2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2985https://doaj.org/toc/2073-4409Psoriasis (Ps), commonly perceived as a skin and joint disorder, has a complex basis and results from disturbances in the sophisticated network between skin and the immune system. This makes it difficult to properly depict the complete pathomechanism on an in vitro scale. Deciphering the complicated or even subtle modulation of intra- and intercellular factors, assisted by the implementation of in vitro human skin models, may provide the opportunity to dissect the disease background step by step. In addition to reconstructed artificial skin substitutes, which mimic the native physiological context, in vitro models are conducive to the broad “3 Rs” philosophy (reduce, refine, and replace) and represent important tools for basic and applied skin research. To meet the need for a more comprehensive in vitro Ps model, a set of various experimental conditions was applied in this study. The selection of in vitro treatment that mimicked the Ps phenotype was illustrated by analyses of discriminating biomarker genes involved in the pathogenesis of the disease, i.e., keratinocyte differentiation markers, antimicrobial peptides, chemokines, and proliferation markers. This resulted in a reproducible protocol for the use of the primary skin keratinocyte (pKC) monoculture treated with a cytokine cocktail (5MIX, i.e., interleukin (IL) 1 alpha (IL-1α), IL-17A, IL-22, oncostatin M (OSM), and tumour necrosis factor alpha (TNF-α)) at a calcium (Ca<sup>2+</sup>) concentration (i.e., 2 mM) in an applied medium, which best mirrored the in vitro Ps-like inflammatory model. In addition, based on waste skin material, the method has the potential for extensive experimentation, both in detailed molecular studies and preclinical tests.Katarzyna BocheńskaMarta MoskotMagdalena Gabig-CimińskaMDPI AGarticlecell-based modelsin vitro culturesskinepidermal keratinocytesmonocytespsoriasisBiology (General)QH301-705.5ENCells, Vol 10, Iss 2985, p 2985 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell-based models in vitro cultures skin epidermal keratinocytes monocytes psoriasis Biology (General) QH301-705.5
spellingShingle	cell-based models in vitro cultures skin epidermal keratinocytes monocytes psoriasis Biology (General) QH301-705.5 Katarzyna Bocheńska Marta Moskot Magdalena Gabig-Cimińska Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
description	Psoriasis (Ps), commonly perceived as a skin and joint disorder, has a complex basis and results from disturbances in the sophisticated network between skin and the immune system. This makes it difficult to properly depict the complete pathomechanism on an in vitro scale. Deciphering the complicated or even subtle modulation of intra- and intercellular factors, assisted by the implementation of in vitro human skin models, may provide the opportunity to dissect the disease background step by step. In addition to reconstructed artificial skin substitutes, which mimic the native physiological context, in vitro models are conducive to the broad “3 Rs” philosophy (reduce, refine, and replace) and represent important tools for basic and applied skin research. To meet the need for a more comprehensive in vitro Ps model, a set of various experimental conditions was applied in this study. The selection of in vitro treatment that mimicked the Ps phenotype was illustrated by analyses of discriminating biomarker genes involved in the pathogenesis of the disease, i.e., keratinocyte differentiation markers, antimicrobial peptides, chemokines, and proliferation markers. This resulted in a reproducible protocol for the use of the primary skin keratinocyte (pKC) monoculture treated with a cytokine cocktail (5MIX, i.e., interleukin (IL) 1 alpha (IL-1α), IL-17A, IL-22, oncostatin M (OSM), and tumour necrosis factor alpha (TNF-α)) at a calcium (Ca<sup>2+</sup>) concentration (i.e., 2 mM) in an applied medium, which best mirrored the in vitro Ps-like inflammatory model. In addition, based on waste skin material, the method has the potential for extensive experimentation, both in detailed molecular studies and preclinical tests.
format	article
author	Katarzyna Bocheńska Marta Moskot Magdalena Gabig-Cimińska
author_facet	Katarzyna Bocheńska Marta Moskot Magdalena Gabig-Cimińska
author_sort	Katarzyna Bocheńska
title	Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
title_short	Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
title_full	Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
title_fullStr	Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
title_full_unstemmed	Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
title_sort	use of cytokine mix-, imiquimod-, and serum-induced monoculture and lipopolysaccharide- and interferon gamma-treated co-culture to establish in vitro psoriasis-like inflammation models
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f3e77c1011044c06b84d77aa25a2fccd
work_keys_str_mv	AT katarzynabochenska useofcytokinemiximiquimodandseruminducedmonocultureandlipopolysaccharideandinterferongammatreatedcoculturetoestablishinvitropsoriasislikeinflammationmodels AT martamoskot useofcytokinemiximiquimodandseruminducedmonocultureandlipopolysaccharideandinterferongammatreatedcoculturetoestablishinvitropsoriasislikeinflammationmodels AT magdalenagabigciminska useofcytokinemiximiquimodandseruminducedmonocultureandlipopolysaccharideandinterferongammatreatedcoculturetoestablishinvitropsoriasislikeinflammationmodels
_version_	1718412688651976704

Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models

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