Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

Wei-Juan Zeng,1 Chun-Wei Peng,1 Jing-Ping Yuan,1 Ran Cui,2 Yan Li11Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People’s Republic of China; 2Key Laboratory of Analy...

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Autores principales: Zeng WJ, Peng CW, Yuan JP, Cui R, Li Y
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/f3e8bf24c6e441d9947d00d1bbc9af27
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spelling oai:doaj.org-article:f3e8bf24c6e441d9947d00d1bbc9af272021-12-02T01:38:46ZQuantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification1178-2013https://doaj.org/article/f3e8bf24c6e441d9947d00d1bbc9af272015-03-01T00:00:00Zhttp://www.dovepress.com/quantum-dot-based-multiplexed-imaging-innbspmalignant-ascites-a-new-mo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Wei-Juan Zeng,1 Chun-Wei Peng,1 Jing-Ping Yuan,1 Ran Cui,2 Yan Li11Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People’s Republic of China; 2Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) and College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People’s Republic of China Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized. Keywords: gastric cancer, malignant ascites, quantum dots, classification, multiplexed imagingZeng WJPeng CWYuan JPCui RLi YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1759-1768 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Zeng WJ
Peng CW
Yuan JP
Cui R
Li Y
Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
description Wei-Juan Zeng,1 Chun-Wei Peng,1 Jing-Ping Yuan,1 Ran Cui,2 Yan Li11Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan, People’s Republic of China; 2Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) and College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People’s Republic of China Purpose: The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification.Methods: A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features.Results: The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes (P<0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer.Conclusion: The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized. Keywords: gastric cancer, malignant ascites, quantum dots, classification, multiplexed imaging
format article
author Zeng WJ
Peng CW
Yuan JP
Cui R
Li Y
author_facet Zeng WJ
Peng CW
Yuan JP
Cui R
Li Y
author_sort Zeng WJ
title Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
title_short Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
title_full Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
title_fullStr Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
title_full_unstemmed Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
title_sort quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/f3e8bf24c6e441d9947d00d1bbc9af27
work_keys_str_mv AT zengwj quantumdotbasedmultiplexedimaginginnbspmalignantascitesanewmodelformalignantascitesclassification
AT pengcw quantumdotbasedmultiplexedimaginginnbspmalignantascitesanewmodelformalignantascitesclassification
AT yuanjp quantumdotbasedmultiplexedimaginginnbspmalignantascitesanewmodelformalignantascitesclassification
AT cuir quantumdotbasedmultiplexedimaginginnbspmalignantascitesanewmodelformalignantascitesclassification
AT liy quantumdotbasedmultiplexedimaginginnbspmalignantascitesanewmodelformalignantascitesclassification
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