Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.

Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little...

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Autores principales:	Ligang Wang, Hongkuan Yang, Shiguang Zhao, Haruhisa Sato, Yoshihiro Konishi, Thomas G Beach, Essam Mohamed Abdelalim, Naomi J Bisem, Ikuo Tooyama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3e9cbc16636427e8b7915d03519a728
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spelling	oai:doaj.org-article:f3e9cbc16636427e8b7915d03519a7282021-11-18T06:49:51ZExpression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.1932-620310.1371/journal.pone.0022325https://doaj.org/article/f3e9cbc16636427e8b7915d03519a7282011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21799823/?tool=EBIhttps://doaj.org/toc/1932-6203Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H(2)O(2) was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.Ligang WangHongkuan YangShiguang ZhaoHaruhisa SatoYoshihiro KonishiThomas G BeachEssam Mohamed AbdelalimNaomi J BisemIkuo TooyamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22325 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ligang Wang Hongkuan Yang Shiguang Zhao Haruhisa Sato Yoshihiro Konishi Thomas G Beach Essam Mohamed Abdelalim Naomi J Bisem Ikuo Tooyama Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
description	Mitochondrial ferritin (MtF) has been identified as a novel ferritin encoded by an intron-lacking gene with specific mitochondrial localization located on chromosome 5q23.1. MtF has been associated with neurodegenerative disorders such as Friedreich ataxia and restless leg syndrome. However, little information is available about MtF in Alzheimer's disease (AD). In this study, therefore, we investigated the expression and localization of MtF messenger RNA (mRNA) in the cerebral cortex of AD and control cases using real-time polymerase chain reaction (PCR) as well as in situ hybridization histochemistry. We also examined protein expression using western-blot assay. In addition, we used in vitro methods to further explore the effect of oxidative stress and β-amyloid peptide (Aβ) on MtF expression. To do this we examined MtF mRNA and protein expression changes in the human neuroblastoma cell line, IMR-32, after treatment with Aβ, H2O2, or both. The neuroprotective effect of MtF on oxidative stress induced by H(2)O(2) was measured by MTT assay. The in situ hybridization studies revealed that MtF mRNA was detected mainly in neurons to a lesser degree in glial cells in the cerebral cortex. The staining intensity and the number of positive cells were increased in the cerebral cortex of AD patients. Real-time PCR and western-blot confirmed that MtF expression levels in the cerebral cortex were significantly higher in AD cases than that in control cases at both the mRNA and the protein level. Cell culture experiments demonstrated that the expression of both MtF mRNA and protein were increased by treatment with H2O2 or a combination of Aβ and H2O2, but not with Aβ alone. Finally, MtF expression showed a significant neuroprotective effect against H2O2-induced oxidative stress (p<0.05). The present study suggests that MtF is involved in the pathology of AD and may play a neuroprotective role against oxidative stress.
format	article
author	Ligang Wang Hongkuan Yang Shiguang Zhao Haruhisa Sato Yoshihiro Konishi Thomas G Beach Essam Mohamed Abdelalim Naomi J Bisem Ikuo Tooyama
author_facet	Ligang Wang Hongkuan Yang Shiguang Zhao Haruhisa Sato Yoshihiro Konishi Thomas G Beach Essam Mohamed Abdelalim Naomi J Bisem Ikuo Tooyama
author_sort	Ligang Wang
title	Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
title_short	Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
title_full	Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
title_fullStr	Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
title_full_unstemmed	Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.
title_sort	expression and localization of mitochondrial ferritin mrna in alzheimer's disease cerebral cortex.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f3e9cbc16636427e8b7915d03519a728
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_version_	1718424391290716160

Expression and localization of mitochondrial ferritin mRNA in Alzheimer's disease cerebral cortex.

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