Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21

ABSTRACT: The presence of Salmonella in air of poultry houses has been previously confirmed. Therefore, it is important to investigate the entry of Salmonella into broilers through air. The present study aimed to evaluate different levels of Salmonella Enteritidis aerosol inoculations in broiler chi...

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Autores principales:	Amrit Pal, Montana R. Riggs, Andrea Urrutia, Rachel C. Osborne, Alexandra P. Jackson, Matthew A. Bailey, Kenneth S. Macklin, Stuart B. Price, R. Jeffrey Buhr, Dianna V. Bourassa
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Salmonella broiler aerosol tissue poultry Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/f3ea8a1ee42f4d90ad3a0115f9b7c985
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spelling	oai:doaj.org-article:f3ea8a1ee42f4d90ad3a0115f9b7c9852021-11-24T04:22:44ZInvestigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 210032-579110.1016/j.psj.2021.101504https://doaj.org/article/f3ea8a1ee42f4d90ad3a0115f9b7c9852021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005265https://doaj.org/toc/0032-5791ABSTRACT: The presence of Salmonella in air of poultry houses has been previously confirmed. Therefore, it is important to investigate the entry of Salmonella into broilers through air. The present study aimed to evaluate different levels of Salmonella Enteritidis aerosol inoculations in broiler chicks for colonization of ceca, trachea, and liver/spleen and persistence over time. In 3 independent trials, 112 one-day-old birds were randomly divided into 4 groups (n = 28/group). On d 1 of age, one group was exposed to an aerosol of sterile saline and the remaining three groups were exposed to an aerosol generated from one of 3 doses (103, 106, or 109 CFU/mL) of S. Enteritidis inoculum. Aerosol exposure time was 30 min/group and was performed using a nebulizer. On d 3, 7, 14, and 21 of age, ceca, trachea, and liver/spleen were aseptically removed. Ceca were cultured for Salmonella counts (log10 CFU/g) and all tissues were cultured for Salmonella prevalence. All tissues from the control group were Salmonella negative for all sampling days. On sampling d 3 and 7, ceca Salmonella counts were highest (5.14 and 5.11, respectively) when challenged with 109 Salmonella (P ≤ 0.0281). Ceca Salmonella counts increased from d 3 (2.43) to d 7 (4.43), then remained constant when challenged at 103 Salmonella, and counts decreased over time for all other groups. Tissue Salmonella prevalence increased with increasing challenge levels at all sampling timepoints (P ≤ 0.0213). Salmonella prevalence was low (0/18 to 4/18) and did not change over time following 103 Salmonella challenge (P ≥ 0.2394). Prevalence decreased over time in ceca and trachea following 106 and 109 Salmonella challenge (P ≤ 0.0483). Liver/spleen Salmonella prevalence increased from d 3 (13/18) to d 14 (18/18) and then decreased at d 21 (10/18) in birds exposed to an aerosol of 109 Salmonella but remained constant over time for rest of the Salmonella inoculated groups. Overall, this study demonstrated the Salmonella colonization and persistence in different tissues in broilers following exposure to aerosolized Salmonella.Amrit PalMontana R. RiggsAndrea UrrutiaRachel C. OsborneAlexandra P. JacksonMatthew A. BaileyKenneth S. MacklinStuart B. PriceR. Jeffrey BuhrDianna V. BourassaElsevierarticleSalmonellabroileraerosoltissuepoultryAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101504- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella broiler aerosol tissue poultry Animal culture SF1-1100
spellingShingle	Salmonella broiler aerosol tissue poultry Animal culture SF1-1100 Amrit Pal Montana R. Riggs Andrea Urrutia Rachel C. Osborne Alexandra P. Jackson Matthew A. Bailey Kenneth S. Macklin Stuart B. Price R. Jeffrey Buhr Dianna V. Bourassa Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
description	ABSTRACT: The presence of Salmonella in air of poultry houses has been previously confirmed. Therefore, it is important to investigate the entry of Salmonella into broilers through air. The present study aimed to evaluate different levels of Salmonella Enteritidis aerosol inoculations in broiler chicks for colonization of ceca, trachea, and liver/spleen and persistence over time. In 3 independent trials, 112 one-day-old birds were randomly divided into 4 groups (n = 28/group). On d 1 of age, one group was exposed to an aerosol of sterile saline and the remaining three groups were exposed to an aerosol generated from one of 3 doses (103, 106, or 109 CFU/mL) of S. Enteritidis inoculum. Aerosol exposure time was 30 min/group and was performed using a nebulizer. On d 3, 7, 14, and 21 of age, ceca, trachea, and liver/spleen were aseptically removed. Ceca were cultured for Salmonella counts (log10 CFU/g) and all tissues were cultured for Salmonella prevalence. All tissues from the control group were Salmonella negative for all sampling days. On sampling d 3 and 7, ceca Salmonella counts were highest (5.14 and 5.11, respectively) when challenged with 109 Salmonella (P ≤ 0.0281). Ceca Salmonella counts increased from d 3 (2.43) to d 7 (4.43), then remained constant when challenged at 103 Salmonella, and counts decreased over time for all other groups. Tissue Salmonella prevalence increased with increasing challenge levels at all sampling timepoints (P ≤ 0.0213). Salmonella prevalence was low (0/18 to 4/18) and did not change over time following 103 Salmonella challenge (P ≥ 0.2394). Prevalence decreased over time in ceca and trachea following 106 and 109 Salmonella challenge (P ≤ 0.0483). Liver/spleen Salmonella prevalence increased from d 3 (13/18) to d 14 (18/18) and then decreased at d 21 (10/18) in birds exposed to an aerosol of 109 Salmonella but remained constant over time for rest of the Salmonella inoculated groups. Overall, this study demonstrated the Salmonella colonization and persistence in different tissues in broilers following exposure to aerosolized Salmonella.
format	article
author	Amrit Pal Montana R. Riggs Andrea Urrutia Rachel C. Osborne Alexandra P. Jackson Matthew A. Bailey Kenneth S. Macklin Stuart B. Price R. Jeffrey Buhr Dianna V. Bourassa
author_facet	Amrit Pal Montana R. Riggs Andrea Urrutia Rachel C. Osborne Alexandra P. Jackson Matthew A. Bailey Kenneth S. Macklin Stuart B. Price R. Jeffrey Buhr Dianna V. Bourassa
author_sort	Amrit Pal
title	Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
title_short	Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
title_full	Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
title_fullStr	Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
title_full_unstemmed	Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21
title_sort	investigation of the potential of aerosolized salmonella enteritidis on colonization and persistence in broilers from day 3 to 21
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f3ea8a1ee42f4d90ad3a0115f9b7c985
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Investigation of the potential of aerosolized Salmonella Enteritidis on colonization and persistence in broilers from day 3 to 21

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