Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei

RNA interference (RNAi) is a conservative and important functional pathway in eukaryocyte. It regulates the expression of genes that are engaged in a variety of cellular physiological functions. Among the functions of RNAi, its antiviral function have attracted many attentions.The RNAi pathway molec...

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Autores principales:	Bin-Bin Li, Jin-Quan Fan, Ke-Cheng Lu, Guo-Liang Chen, Yi-Hong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Litopenaeus vannamei RNA interference Systemic RNA interference defective 1 White spot syndrome virus Zoology QL1-991
Acceso en línea:	https://doaj.org/article/f3ed52597dd643f997ee5d600806865f
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spelling	oai:doaj.org-article:f3ed52597dd643f997ee5d600806865f2021-11-20T05:15:47ZIdentification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei2667-011910.1016/j.fsirep.2021.100033https://doaj.org/article/f3ed52597dd643f997ee5d600806865f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667011921000281https://doaj.org/toc/2667-0119RNA interference (RNAi) is a conservative and important functional pathway in eukaryocyte. It regulates the expression of genes that are engaged in a variety of cellular physiological functions. Among the functions of RNAi, its antiviral function have attracted many attentions.The RNAi pathway molecules are able to recognize virus-related dsRNA and degrade it, therefore killing the virus. More importantly, RNAi could mediate systemic antiviral responses, transmit from cell to cell, and systemic RNA interference defective 1 (SID1) was thought to play an important role in this process. In the present study, a SID1 gene (LvSID1) of Litopenaeus vannamei was cloned. LvSID1 could locate to both plasma membrane and endoplasmic reticulum. Result of real-time RT-PCR assay showed that it was highly expressed in shrimp gills. Besides, it was shown that over-expressed LvSID1 in Sf9 cells could significant enchane RNAi efficiency. It was found that the expression of LvSID1was regulated by white spot syndrome virus (WSSV), and knockdown expression of LvSID1 increased the cumulative mortality of WSSV infection shrimp. These results suggested that LvSID1 likely to played a role in L. vannamei systemic RNAi, and was involved in WSSV resistence.Bin-Bin LiJin-Quan FanKe-Cheng LuGuo-Liang ChenYi-Hong ChenElsevierarticleLitopenaeus vannameiRNA interferenceSystemic RNA interference defective 1White spot syndrome virusZoologyQL1-991ENFish and Shellfish Immunology Reports, Vol 2, Iss , Pp 100033- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Litopenaeus vannamei RNA interference Systemic RNA interference defective 1 White spot syndrome virus Zoology QL1-991
spellingShingle	Litopenaeus vannamei RNA interference Systemic RNA interference defective 1 White spot syndrome virus Zoology QL1-991 Bin-Bin Li Jin-Quan Fan Ke-Cheng Lu Guo-Liang Chen Yi-Hong Chen Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
description	RNA interference (RNAi) is a conservative and important functional pathway in eukaryocyte. It regulates the expression of genes that are engaged in a variety of cellular physiological functions. Among the functions of RNAi, its antiviral function have attracted many attentions.The RNAi pathway molecules are able to recognize virus-related dsRNA and degrade it, therefore killing the virus. More importantly, RNAi could mediate systemic antiviral responses, transmit from cell to cell, and systemic RNA interference defective 1 (SID1) was thought to play an important role in this process. In the present study, a SID1 gene (LvSID1) of Litopenaeus vannamei was cloned. LvSID1 could locate to both plasma membrane and endoplasmic reticulum. Result of real-time RT-PCR assay showed that it was highly expressed in shrimp gills. Besides, it was shown that over-expressed LvSID1 in Sf9 cells could significant enchane RNAi efficiency. It was found that the expression of LvSID1was regulated by white spot syndrome virus (WSSV), and knockdown expression of LvSID1 increased the cumulative mortality of WSSV infection shrimp. These results suggested that LvSID1 likely to played a role in L. vannamei systemic RNAi, and was involved in WSSV resistence.
format	article
author	Bin-Bin Li Jin-Quan Fan Ke-Cheng Lu Guo-Liang Chen Yi-Hong Chen
author_facet	Bin-Bin Li Jin-Quan Fan Ke-Cheng Lu Guo-Liang Chen Yi-Hong Chen
author_sort	Bin-Bin Li
title	Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
title_short	Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
title_full	Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
title_fullStr	Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
title_full_unstemmed	Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei
title_sort	identification and functional characterization of a systemic rna interference defective 1 gene in litopenaeus vannamei
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f3ed52597dd643f997ee5d600806865f
work_keys_str_mv	AT binbinli identificationandfunctionalcharacterizationofasystemicrnainterferencedefective1geneinlitopenaeusvannamei AT jinquanfan identificationandfunctionalcharacterizationofasystemicrnainterferencedefective1geneinlitopenaeusvannamei AT kechenglu identificationandfunctionalcharacterizationofasystemicrnainterferencedefective1geneinlitopenaeusvannamei AT guoliangchen identificationandfunctionalcharacterizationofasystemicrnainterferencedefective1geneinlitopenaeusvannamei AT yihongchen identificationandfunctionalcharacterizationofasystemicrnainterferencedefective1geneinlitopenaeusvannamei
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Identification and functional characterization of a systemic RNA interference defective 1 gene in Litopenaeus vannamei

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