Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges

Limited by the poor proliferation and restricted sources of adult hepatocytes, there is an urgent need to find substitutes for proliferation and cultivation of mature hepatocytes in vitro for use in disease treatment, drug approval, and toxicity testing. Hepatocyte-like cells (HLCs), which originate...

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Autores principales:	Ye Xie, Jia Yao, Weilin Jin, Longfei Ren, Xun Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hepatocyte-like cells hepatocyte induction chemical approach culture system genetic manipulation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f409426274a444a2bc95cb85113ce789
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spelling	oai:doaj.org-article:f409426274a444a2bc95cb85113ce7892021-12-01T08:35:16ZInduction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges2296-634X10.3389/fcell.2021.765980https://doaj.org/article/f409426274a444a2bc95cb85113ce7892021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.765980/fullhttps://doaj.org/toc/2296-634XLimited by the poor proliferation and restricted sources of adult hepatocytes, there is an urgent need to find substitutes for proliferation and cultivation of mature hepatocytes in vitro for use in disease treatment, drug approval, and toxicity testing. Hepatocyte-like cells (HLCs), which originate from undifferentiated stem cells or modified adult cells, are considered good candidates because of their advantages in terms of cell source and in vitro expansion ability. However, the majority of induced HLCs are in an immature state, and their degree of differentiation is heterogeneous, diminishing their usability in basic research and limiting their clinical application. Therefore, various methods have been developed to promote the maturation of HLCs, including chemical approaches, alteration of cell culture systems, and genetic manipulation, to meet the needs of in vivo transplantation and in vitro model establishment. This review proposes different cell types for the induction of HLCs, and provide a comprehensive overview of various techniques to promote the generation and maturation of HLCs in vitro.Ye XieJia YaoJia YaoWeilin JinWeilin JinWeilin JinLongfei RenLongfei RenXun LiXun LiXun LiXun LiXun LiFrontiers Media S.A.articlehepatocyte-like cellshepatocyte inductionchemical approachculture systemgenetic manipulationBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatocyte-like cells hepatocyte induction chemical approach culture system genetic manipulation Biology (General) QH301-705.5
spellingShingle	hepatocyte-like cells hepatocyte induction chemical approach culture system genetic manipulation Biology (General) QH301-705.5 Ye Xie Jia Yao Jia Yao Weilin Jin Weilin Jin Weilin Jin Longfei Ren Longfei Ren Xun Li Xun Li Xun Li Xun Li Xun Li Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
description	Limited by the poor proliferation and restricted sources of adult hepatocytes, there is an urgent need to find substitutes for proliferation and cultivation of mature hepatocytes in vitro for use in disease treatment, drug approval, and toxicity testing. Hepatocyte-like cells (HLCs), which originate from undifferentiated stem cells or modified adult cells, are considered good candidates because of their advantages in terms of cell source and in vitro expansion ability. However, the majority of induced HLCs are in an immature state, and their degree of differentiation is heterogeneous, diminishing their usability in basic research and limiting their clinical application. Therefore, various methods have been developed to promote the maturation of HLCs, including chemical approaches, alteration of cell culture systems, and genetic manipulation, to meet the needs of in vivo transplantation and in vitro model establishment. This review proposes different cell types for the induction of HLCs, and provide a comprehensive overview of various techniques to promote the generation and maturation of HLCs in vitro.
format	article
author	Ye Xie Jia Yao Jia Yao Weilin Jin Weilin Jin Weilin Jin Longfei Ren Longfei Ren Xun Li Xun Li Xun Li Xun Li Xun Li
author_facet	Ye Xie Jia Yao Jia Yao Weilin Jin Weilin Jin Weilin Jin Longfei Ren Longfei Ren Xun Li Xun Li Xun Li Xun Li Xun Li
author_sort	Ye Xie
title	Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
title_short	Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
title_full	Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
title_fullStr	Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
title_full_unstemmed	Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
title_sort	induction and maturation of hepatocyte-like cells in vitro: focus on technological advances and challenges
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f409426274a444a2bc95cb85113ce789
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Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges

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