Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins

Abstract Here we describe a new family of carotenoid cleavage oxygenases (CCOs) in metazoans, the BCO2-like (BCOL) clade, which contains lancelet, nematode, and molluscan carotenoid oxygenase sequences. Phylogenetic analysis of CCOs in all kingdoms of life confirmed that the BCOL enzymes are an inde...

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Autores principales:	Eugenia Poliakov, Joseph Soucy, Susan Gentleman, Igor B. Rogozin, T. Michael Redmond
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f411c555a86b4c2490a23365c5dbfb0d
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spelling	oai:doaj.org-article:f411c555a86b4c2490a23365c5dbfb0d2021-12-02T15:06:25ZPhylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins10.1038/s41598-017-13521-x2045-2322https://doaj.org/article/f411c555a86b4c2490a23365c5dbfb0d2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13521-xhttps://doaj.org/toc/2045-2322Abstract Here we describe a new family of carotenoid cleavage oxygenases (CCOs) in metazoans, the BCO2-like (BCOL) clade, which contains lancelet, nematode, and molluscan carotenoid oxygenase sequences. Phylogenetic analysis of CCOs in all kingdoms of life confirmed that the BCOL enzymes are an independent clade of ancient origin. One of the predicted lancelet BCOL proteins, cloned and analyzed for carotenoid cleavage activity in a bacterial carotenoid expression system, had activity similar to lancelet BCO2 proteins, although with a preference for cis isomers. Our docking predictions correlated well with the cis-favored activity. The extensive expansions of the new animal BCOL family in some species (e.g., lancelet) suggests that the carotenoid cleavage oxygenase superfamily has evolved in the “extremely high turnover” fashion: numerous losses and duplications of this family are likely to reflect complex regulation processes during development, and interactions with the environment. These findings also serve to provide a rationale for the evolution of the BCO-related outlier RPE65 retinol isomerase, an enzyme that does not utilize carotenoids as substrate or perform double-bond cleavage.Eugenia PoliakovJoseph SoucySusan GentlemanIgor B. RogozinT. Michael RedmondNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eugenia Poliakov Joseph Soucy Susan Gentleman Igor B. Rogozin T. Michael Redmond Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
description	Abstract Here we describe a new family of carotenoid cleavage oxygenases (CCOs) in metazoans, the BCO2-like (BCOL) clade, which contains lancelet, nematode, and molluscan carotenoid oxygenase sequences. Phylogenetic analysis of CCOs in all kingdoms of life confirmed that the BCOL enzymes are an independent clade of ancient origin. One of the predicted lancelet BCOL proteins, cloned and analyzed for carotenoid cleavage activity in a bacterial carotenoid expression system, had activity similar to lancelet BCO2 proteins, although with a preference for cis isomers. Our docking predictions correlated well with the cis-favored activity. The extensive expansions of the new animal BCOL family in some species (e.g., lancelet) suggests that the carotenoid cleavage oxygenase superfamily has evolved in the “extremely high turnover” fashion: numerous losses and duplications of this family are likely to reflect complex regulation processes during development, and interactions with the environment. These findings also serve to provide a rationale for the evolution of the BCO-related outlier RPE65 retinol isomerase, an enzyme that does not utilize carotenoids as substrate or perform double-bond cleavage.
format	article
author	Eugenia Poliakov Joseph Soucy Susan Gentleman Igor B. Rogozin T. Michael Redmond
author_facet	Eugenia Poliakov Joseph Soucy Susan Gentleman Igor B. Rogozin T. Michael Redmond
author_sort	Eugenia Poliakov
title	Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
title_short	Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
title_full	Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
title_fullStr	Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
title_full_unstemmed	Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins
title_sort	phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of bco-like (bcol) proteins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f411c555a86b4c2490a23365c5dbfb0d
work_keys_str_mv	AT eugeniapoliakov phylogeneticanalysisofthemetazoancarotenoidoxygenasesuperfamilyanewancestralgeneassemblageofbcolikebcolproteins AT josephsoucy phylogeneticanalysisofthemetazoancarotenoidoxygenasesuperfamilyanewancestralgeneassemblageofbcolikebcolproteins AT susangentleman phylogeneticanalysisofthemetazoancarotenoidoxygenasesuperfamilyanewancestralgeneassemblageofbcolikebcolproteins AT igorbrogozin phylogeneticanalysisofthemetazoancarotenoidoxygenasesuperfamilyanewancestralgeneassemblageofbcolikebcolproteins AT tmichaelredmond phylogeneticanalysisofthemetazoancarotenoidoxygenasesuperfamilyanewancestralgeneassemblageofbcolikebcolproteins
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Phylogenetic analysis of the metazoan carotenoid oxygenase superfamily: a new ancestral gene assemblage of BCO-like (BCOL) proteins

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