Transgenes of genetically modified animals detected non-invasively via environmental DNA.

We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of e...

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Autores principales:	Charles C Y Xu, Claire Ramsay, Mitra Cowan, Mehrnoush Dehghani, Paul Lasko, Rowan D H Barrett
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f412641795c4418d91b2e1b2333a0934
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spelling	oai:doaj.org-article:f412641795c4418d91b2e1b2333a09342021-12-02T20:14:54ZTransgenes of genetically modified animals detected non-invasively via environmental DNA.1932-620310.1371/journal.pone.0249439https://doaj.org/article/f412641795c4418d91b2e1b2333a09342021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249439https://doaj.org/toc/1932-6203We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of environmental DNA extracted from food media of GM fruit flies (Drosophila melanogaster), feces, urine, and saliva of GM laboratory mice (Mus musculus), and aquarium water of GM tetra fish (Gymnocorymbus ternetzi). With rapidly growing accessibility of genome-editing technologies such as CRISPR, the prevalence and diversity of GM animals will increase dramatically. GM animals have already been released into the wild with more releases planned in the future. eDNA methods have the potential to address the critical need for sensitive, accurate, and cost-effective detection and monitoring of GM animals and their transgenes in nature.Charles C Y XuClaire RamsayMitra CowanMehrnoush DehghaniPaul LaskoRowan D H BarrettPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0249439 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charles C Y Xu Claire Ramsay Mitra Cowan Mehrnoush Dehghani Paul Lasko Rowan D H Barrett Transgenes of genetically modified animals detected non-invasively via environmental DNA.
description	We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of environmental DNA extracted from food media of GM fruit flies (Drosophila melanogaster), feces, urine, and saliva of GM laboratory mice (Mus musculus), and aquarium water of GM tetra fish (Gymnocorymbus ternetzi). With rapidly growing accessibility of genome-editing technologies such as CRISPR, the prevalence and diversity of GM animals will increase dramatically. GM animals have already been released into the wild with more releases planned in the future. eDNA methods have the potential to address the critical need for sensitive, accurate, and cost-effective detection and monitoring of GM animals and their transgenes in nature.
format	article
author	Charles C Y Xu Claire Ramsay Mitra Cowan Mehrnoush Dehghani Paul Lasko Rowan D H Barrett
author_facet	Charles C Y Xu Claire Ramsay Mitra Cowan Mehrnoush Dehghani Paul Lasko Rowan D H Barrett
author_sort	Charles C Y Xu
title	Transgenes of genetically modified animals detected non-invasively via environmental DNA.
title_short	Transgenes of genetically modified animals detected non-invasively via environmental DNA.
title_full	Transgenes of genetically modified animals detected non-invasively via environmental DNA.
title_fullStr	Transgenes of genetically modified animals detected non-invasively via environmental DNA.
title_full_unstemmed	Transgenes of genetically modified animals detected non-invasively via environmental DNA.
title_sort	transgenes of genetically modified animals detected non-invasively via environmental dna.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f412641795c4418d91b2e1b2333a0934
work_keys_str_mv	AT charlescyxu transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna AT claireramsay transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna AT mitracowan transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna AT mehrnoushdehghani transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna AT paullasko transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna AT rowandhbarrett transgenesofgeneticallymodifiedanimalsdetectednoninvasivelyviaenvironmentaldna
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Transgenes of genetically modified animals detected non-invasively via environmental DNA.

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