mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
Abstract. Chemokines are a key factor that ensures the participation of different cell types in the immunological protection of mucosa. In our study we chose some chemokines that ensured the chemotaxis of neutrophils (CXCL8/IL-8), eosinophils (CCL11/eotaxin, CCL24/eotaxin-...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
SPb RAACI
2014
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/f41c97009952478d91c39c280b7f6aa1 |
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| Sumario: | Abstract. Chemokines are a key factor that ensures the participation of different cell types in the immunological protection of mucosa. In our study we chose some chemokines that ensured the chemotaxis of neutrophils (CXCL8/IL-8), eosinophils (CCL11/eotaxin, CCL24/eotaxin-2), monocytes and T-lymphocytes (CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES), as well as their receptors (CCR1, CCR3, CCR5, CXCR1, CXCR2). mRNA expression of chemokines and their receptors in nasopharyngeal mucosa brush-biopsy specimens determined by RT-PCR in healthy persons, the level of the same chemokines in serum determined by multiplex chemiluminescent assay were analyzed according to smoking. The level of mRNA expression of IL-8 (p < 0.001) and RANTES (p < 0.001) in nasopharynx brush-biopsy specimens and serum levels of IL-8 (p < 0.0001) of smokers were significantly lower as compared with nonsmokers. Correlation analysis showed the dependence of the chemokine synthesis on the factor of smoking: the index of smoking (pack/years) is negatively correlated with mRNA levels of IL-8 (r = -0,67 p = 0,003) and RANTES (r = -0,58, p = 0,015) in nasopharynx brush-biopsy specimens and serum concentration of IL-8 (r = -0,89, p = 0,0000002). Thus, these data offer that smokers manifested a defect of the local synthesis of RANTES and IL-8 in nasopharyngeal mucosa in combination with systemic defect of IL-8 production in peripheral blood, that can lead to chronization of bacterial infection and prolonged persistence of viral infection. (Med. Immunol., 2011, vol. 13, N 6, pp 617-622) |
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