mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS

Abstract. Chemokines are a key factor that ensures the participation of different cell types in the immunological protection of mucosa. In our study we chose some chemokines that ensured the chemotaxis of neutrophils (CXCL8/IL-8), eosinophils (CCL11/eotaxin, CCL24/eotaxin-...

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Autores principales:	A. A. Bibkova, K. A. Sysoyev, A. V. Semenov, N. E. Lyubimova, N. A. Arsentieva, V. I. Trofimov, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	chemokines chemokine receptors mip-1α mip-1β rantes eotaxin eotaxin-2 il-8 smoking Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f41c97009952478d91c39c280b7f6aa1
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spelling	oai:doaj.org-article:f41c97009952478d91c39c280b7f6aa12021-11-18T08:03:42ZmRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS1563-06252313-741X10.15789/1563-0625-2011-6-617-622https://doaj.org/article/f41c97009952478d91c39c280b7f6aa12014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/599https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Chemokines are a key factor that ensures the participation of different cell types in the immunological protection of mucosa. In our study we chose some chemokines that ensured the chemotaxis of neutrophils (CXCL8/IL-8), eosinophils (CCL11/eotaxin, CCL24/eotaxin-2), monocytes and T-lymphocytes (CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES), as well as their receptors (CCR1, CCR3, CCR5, CXCR1, CXCR2). mRNA expression of chemokines and their receptors in nasopharyngeal mucosa brush-biopsy specimens determined by RT-PCR in healthy persons, the level of the same chemokines in serum determined by multiplex chemiluminescent assay were analyzed according to smoking. The level of mRNA expression of IL-8 (p < 0.001) and RANTES (p < 0.001) in nasopharynx brush-biopsy specimens and serum levels of IL-8 (p < 0.0001) of smokers were significantly lower as compared with nonsmokers. Correlation analysis showed the dependence of the chemokine synthesis on the factor of smoking: the index of smoking (pack/years) is negatively correlated with mRNA levels of IL-8 (r = -0,67 p = 0,003) and RANTES (r = -0,58, p = 0,015) in nasopharynx brush-biopsy specimens and serum concentration of IL-8 (r = -0,89, p = 0,0000002). Thus, these data offer that smokers manifested a defect of the local synthesis of RANTES and IL-8 in nasopharyngeal mucosa in combination with systemic defect of IL-8 production in peripheral blood, that can lead to chronization of bacterial infection and prolonged persistence of viral infection. (Med. Immunol., 2011, vol. 13, N 6, pp 617-622)A. A. BibkovaK. A. SysoyevA. V. SemenovN. E. LyubimovaN. A. ArsentievaV. I. TrofimovAreg A. TotolianSPb RAACIarticlechemokineschemokine receptorsmip-1αmip-1βranteseotaxineotaxin-2il-8smokingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 13, Iss 6, Pp 617-622 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	chemokines chemokine receptors mip-1α mip-1β rantes eotaxin eotaxin-2 il-8 smoking Immunologic diseases. Allergy RC581-607
spellingShingle	chemokines chemokine receptors mip-1α mip-1β rantes eotaxin eotaxin-2 il-8 smoking Immunologic diseases. Allergy RC581-607 A. A. Bibkova K. A. Sysoyev A. V. Semenov N. E. Lyubimova N. A. Arsentieva V. I. Trofimov Areg A. Totolian mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
description	Abstract. Chemokines are a key factor that ensures the participation of different cell types in the immunological protection of mucosa. In our study we chose some chemokines that ensured the chemotaxis of neutrophils (CXCL8/IL-8), eosinophils (CCL11/eotaxin, CCL24/eotaxin-2), monocytes and T-lymphocytes (CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES), as well as their receptors (CCR1, CCR3, CCR5, CXCR1, CXCR2). mRNA expression of chemokines and their receptors in nasopharyngeal mucosa brush-biopsy specimens determined by RT-PCR in healthy persons, the level of the same chemokines in serum determined by multiplex chemiluminescent assay were analyzed according to smoking. The level of mRNA expression of IL-8 (p < 0.001) and RANTES (p < 0.001) in nasopharynx brush-biopsy specimens and serum levels of IL-8 (p < 0.0001) of smokers were significantly lower as compared with nonsmokers. Correlation analysis showed the dependence of the chemokine synthesis on the factor of smoking: the index of smoking (pack/years) is negatively correlated with mRNA levels of IL-8 (r = -0,67 p = 0,003) and RANTES (r = -0,58, p = 0,015) in nasopharynx brush-biopsy specimens and serum concentration of IL-8 (r = -0,89, p = 0,0000002). Thus, these data offer that smokers manifested a defect of the local synthesis of RANTES and IL-8 in nasopharyngeal mucosa in combination with systemic defect of IL-8 production in peripheral blood, that can lead to chronization of bacterial infection and prolonged persistence of viral infection. (Med. Immunol., 2011, vol. 13, N 6, pp 617-622)
format	article
author	A. A. Bibkova K. A. Sysoyev A. V. Semenov N. E. Lyubimova N. A. Arsentieva V. I. Trofimov Areg A. Totolian
author_facet	A. A. Bibkova K. A. Sysoyev A. V. Semenov N. E. Lyubimova N. A. Arsentieva V. I. Trofimov Areg A. Totolian
author_sort	A. A. Bibkova
title	mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
title_short	mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
title_full	mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
title_fullStr	mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
title_full_unstemmed	mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS
title_sort	mrna expression of some chemokines and their receptors in nasal mucosa of healthy persons
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/f41c97009952478d91c39c280b7f6aa1
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mRNA EXPRESSION OF SOME CHEMOKINES AND THEIR RECEPTORS IN NASAL MUCOSA OF HEALTHY PERSONS

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