Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India

The diabetic population faces 80% increased risk of cellulitis, 4-fold increased risk of osteomyelitis and 2-fold risk of both sepsis and death caused by infections.Study objectives. The present study was carried out to assess the clinical aspects and microbiological profile of organisms isolated fr...

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Autores principales:	Rajpal Singh Punia, Reetu Kundu, Vikram Jassal, Ashok Kumar Attri, Jagdish Chander
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2015
Materias:	diabetes amputation infection culture Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/f425566cc4cd47be8d238fed543e7bf9
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spelling	oai:doaj.org-article:f425566cc4cd47be8d238fed543e7bf92021-11-14T09:00:19ZClinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India2072-03512072-037810.14341/DM2015279-83https://doaj.org/article/f425566cc4cd47be8d238fed543e7bf92015-06-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/7093https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378The diabetic population faces 80% increased risk of cellulitis, 4-fold increased risk of osteomyelitis and 2-fold risk of both sepsis and death caused by infections.Study objectives. The present study was carried out to assess the clinical aspects and microbiological profile of organisms isolated from 25 patients undergoing diabetic limb amputations.Materials and Methods. In 25 diabetes persons who underwent limb amputation, grading of ulcers was done according to Wagner system. Material was stained with Gram stain. Potassium hydroxide wet mounts were also studied. Culture was done in blood agar, MacConkey agar, Sabouraud dextrose agar tube slants and brain heart infusion broth and examined for growth. The histopathology sections were also studied and special stains were done.Results. Of 25 cases, 16 were males and 9 were females. The age ranged from 30 to 90 years (mean: 58?10.91). Majority of ulcers were grade 3. Osteomyelitis was seen in 13 (52%) cases; acute in 2 (8%), chronic in 3 (12%) and acute exacerbation of chronic osteomyelitis in 8 (32%) cases. On culture Proteus mirabilis was isolated in majority of cases followed by Escherichia coli. In 20 cases more than one bacterium were isolated. Candida was cultured in 8 cases followed by Trichosporon in 2 and Fusarium in one case. On histopathology Candida was seen in 3 cases, while one case showed spores of Trichosporon. 80% cases with osteomyelitis had polymicrobial infection.Conclusions. The isolation of etiologic agent helps in administering appropriate antibiotic regimens, thus reducing the problem of multidrug resistance, morbidity and surgical limb amputations in patients suffering from diabetes mellitus.Rajpal Singh PuniaReetu KunduVikram JassalAshok Kumar AttriJagdish ChanderEndocrinology Research CentrearticlediabetesamputationinfectioncultureNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 18, Iss 2, Pp 79-83 (2015)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	diabetes amputation infection culture Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	diabetes amputation infection culture Nutritional diseases. Deficiency diseases RC620-627 Rajpal Singh Punia Reetu Kundu Vikram Jassal Ashok Kumar Attri Jagdish Chander Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
description	The diabetic population faces 80% increased risk of cellulitis, 4-fold increased risk of osteomyelitis and 2-fold risk of both sepsis and death caused by infections.Study objectives. The present study was carried out to assess the clinical aspects and microbiological profile of organisms isolated from 25 patients undergoing diabetic limb amputations.Materials and Methods. In 25 diabetes persons who underwent limb amputation, grading of ulcers was done according to Wagner system. Material was stained with Gram stain. Potassium hydroxide wet mounts were also studied. Culture was done in blood agar, MacConkey agar, Sabouraud dextrose agar tube slants and brain heart infusion broth and examined for growth. The histopathology sections were also studied and special stains were done.Results. Of 25 cases, 16 were males and 9 were females. The age ranged from 30 to 90 years (mean: 58?10.91). Majority of ulcers were grade 3. Osteomyelitis was seen in 13 (52%) cases; acute in 2 (8%), chronic in 3 (12%) and acute exacerbation of chronic osteomyelitis in 8 (32%) cases. On culture Proteus mirabilis was isolated in majority of cases followed by Escherichia coli. In 20 cases more than one bacterium were isolated. Candida was cultured in 8 cases followed by Trichosporon in 2 and Fusarium in one case. On histopathology Candida was seen in 3 cases, while one case showed spores of Trichosporon. 80% cases with osteomyelitis had polymicrobial infection.Conclusions. The isolation of etiologic agent helps in administering appropriate antibiotic regimens, thus reducing the problem of multidrug resistance, morbidity and surgical limb amputations in patients suffering from diabetes mellitus.
format	article
author	Rajpal Singh Punia Reetu Kundu Vikram Jassal Ashok Kumar Attri Jagdish Chander
author_facet	Rajpal Singh Punia Reetu Kundu Vikram Jassal Ashok Kumar Attri Jagdish Chander
author_sort	Rajpal Singh Punia
title	Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
title_short	Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
title_full	Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
title_fullStr	Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
title_full_unstemmed	Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India
title_sort	clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in north india
publisher	Endocrinology Research Centre
publishDate	2015
url	https://doaj.org/article/f425566cc4cd47be8d238fed543e7bf9
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Clinico-microbiological study of diabetic limb amputations in a tertiary care hospital in North India

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