Chemoenzymatic synthesis of fucosylated oligosaccharides using Thermosynechococcus α1–2-fucosyltransferase and their application in the regulation of intestinal microbiota

Chemoenzymatic synthesis of fucosylated oligosaccharides using Thermosynechococcus α1–2-fucosyltransferase and their application in the regulation of intestinal microbiota

A novel bacterial α 1–2-fucosyltransferase (α 2FT) from Thermosynechococcus sp. NK55a (Ts2FT) has been discovered and characterized. It shares 28–62% protein sequence homology to α 2FTs reported previously. The Ts2FT was cloned as an N-terminal His6-tagged recombinant protein (His6-Ts2FT) and expres...

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Bibliographic Details
Main Authors: Ruting Zhong, Luying Gao, Zhengxin Chen, Sinan Yuan, Xi Chen, Chao Zhao
Format: article
Language:EN
Published: Elsevier 2021
Subjects:
Carbohydrate
Enzymatic synthesis
Fucosyltransferase
Fucosylated oligosaccharides
Lacto-N-fucopentaose I
Thermosynechococcus
Nutrition. Foods and food supply
TX341-641
Food processing and manufacture
TP368-456
Online Access:https://doaj.org/article/f4297c6ef7ae47d6a4e5eee7e47bbace
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