FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species

Abstract The present study explores an alternative method for antioxidants determination in buckwheat (Fagopyrum) samples. Buckwheat contains different amounts of the antioxidants rutin, quercetin and quercitrin in different plant parts. Buckwheat seeds are most commonly used as food; however, prepa...

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Autores principales:	Meta Kokalj Ladan, Janka Straus, Eva Tavčar Benković, Samo Kreft
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f42b78bf2f48425ba5e98b7b56c6235e
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spelling	oai:doaj.org-article:f42b78bf2f48425ba5e98b7b56c6235e2021-12-02T12:31:46ZFT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species10.1038/s41598-017-07665-z2045-2322https://doaj.org/article/f42b78bf2f48425ba5e98b7b56c6235e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07665-zhttps://doaj.org/toc/2045-2322Abstract The present study explores an alternative method for antioxidants determination in buckwheat (Fagopyrum) samples. Buckwheat contains different amounts of the antioxidants rutin, quercetin and quercitrin in different plant parts. Buckwheat seeds are most commonly used as food; however, preparations from the herb can also be used as a rich source of rutin. Infrared spectroscopy was used for individual and sum quantification of rutin, quercetin and quercitrin in whole and ground flowers and leaves of seven different buckwheat species. Correlation coefficients R of calibration and independent validation set for rutin, quercetin and quercitrin were 1.00 and 0.98, 0.94 and 0.99, 0.99 and 0.95, respectively. Some of the developed models had accuracy comparable to the reference HPLC method. Additionally many different parameters that give an important insight into the FTIR technique are discussed (different plant parts, whole and ground untreated samples, 3 different resolutions, 7 spectra pre-treatments, using individual or averaged spectra, reducing spectral data input, considering additional non-spectral data). The implemented technique used no sample preparation, is non-destructive and uses very little amounts of sample. Result show that infrared spectroscopy can be a fast and environmentally friendly alternative technique for routine analysis of main flavonoids in aerial parts of buckwheat.Meta Kokalj LadanJanka StrausEva Tavčar BenkovićSamo KreftNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meta Kokalj Ladan Janka Straus Eva Tavčar Benković Samo Kreft FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
description	Abstract The present study explores an alternative method for antioxidants determination in buckwheat (Fagopyrum) samples. Buckwheat contains different amounts of the antioxidants rutin, quercetin and quercitrin in different plant parts. Buckwheat seeds are most commonly used as food; however, preparations from the herb can also be used as a rich source of rutin. Infrared spectroscopy was used for individual and sum quantification of rutin, quercetin and quercitrin in whole and ground flowers and leaves of seven different buckwheat species. Correlation coefficients R of calibration and independent validation set for rutin, quercetin and quercitrin were 1.00 and 0.98, 0.94 and 0.99, 0.99 and 0.95, respectively. Some of the developed models had accuracy comparable to the reference HPLC method. Additionally many different parameters that give an important insight into the FTIR technique are discussed (different plant parts, whole and ground untreated samples, 3 different resolutions, 7 spectra pre-treatments, using individual or averaged spectra, reducing spectral data input, considering additional non-spectral data). The implemented technique used no sample preparation, is non-destructive and uses very little amounts of sample. Result show that infrared spectroscopy can be a fast and environmentally friendly alternative technique for routine analysis of main flavonoids in aerial parts of buckwheat.
format	article
author	Meta Kokalj Ladan Janka Straus Eva Tavčar Benković Samo Kreft
author_facet	Meta Kokalj Ladan Janka Straus Eva Tavčar Benković Samo Kreft
author_sort	Meta Kokalj Ladan
title	FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
title_short	FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
title_full	FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
title_fullStr	FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
title_full_unstemmed	FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species
title_sort	ft-ir-based method for rutin, quercetin and quercitrin quantification in different buckwheat (fagopyrum) species
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f42b78bf2f48425ba5e98b7b56c6235e
work_keys_str_mv	AT metakokaljladan ftirbasedmethodforrutinquercetinandquercitrinquantificationindifferentbuckwheatfagopyrumspecies AT jankastraus ftirbasedmethodforrutinquercetinandquercitrinquantificationindifferentbuckwheatfagopyrumspecies AT evatavcarbenkovic ftirbasedmethodforrutinquercetinandquercitrinquantificationindifferentbuckwheatfagopyrumspecies AT samokreft ftirbasedmethodforrutinquercetinandquercitrinquantificationindifferentbuckwheatfagopyrumspecies
_version_	1718394313860186112

FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species

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