Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol pho...

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Autores principales:	Mahammed Ilyas Khazi, Chenshuo Li, Fakhra Liaqat, Przemyslaw Malec, Jian Li, Pengcheng Fu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Euryhalinema sp. Desertifilum sp. iron nitrogen C-phycocyanin purification Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f42c4ae4805c4b99a4e137a24a6fa0bd
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spelling	oai:doaj.org-article:f42c4ae4805c4b99a4e137a24a6fa0bd2021-11-10T07:58:37ZAcclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production2296-418510.3389/fbioe.2021.752024https://doaj.org/article/f42c4ae4805c4b99a4e137a24a6fa0bd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.752024/fullhttps://doaj.org/toc/2296-4185This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A620/A280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A620/A280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.Mahammed Ilyas KhaziChenshuo LiFakhra LiaqatPrzemyslaw MalecJian LiPengcheng FuFrontiers Media S.A.articleEuryhalinema sp.Desertifilum sp.ironnitrogenC-phycocyaninpurificationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Euryhalinema sp. Desertifilum sp. iron nitrogen C-phycocyanin purification Biotechnology TP248.13-248.65
spellingShingle	Euryhalinema sp. Desertifilum sp. iron nitrogen C-phycocyanin purification Biotechnology TP248.13-248.65 Mahammed Ilyas Khazi Chenshuo Li Fakhra Liaqat Przemyslaw Malec Jian Li Pengcheng Fu Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
description	This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A620/A280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A620/A280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.
format	article
author	Mahammed Ilyas Khazi Chenshuo Li Fakhra Liaqat Przemyslaw Malec Jian Li Pengcheng Fu
author_facet	Mahammed Ilyas Khazi Chenshuo Li Fakhra Liaqat Przemyslaw Malec Jian Li Pengcheng Fu
author_sort	Mahammed Ilyas Khazi
title	Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_short	Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_full	Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_fullStr	Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_full_unstemmed	Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production
title_sort	acclimation and characterization of marine cyanobacterial strains euryhalinema and desertifilum for c-phycocyanin production
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f42c4ae4805c4b99a4e137a24a6fa0bd
work_keys_str_mv	AT mahammedilyaskhazi acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction AT chenshuoli acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction AT fakhraliaqat acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction AT przemyslawmalec acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction AT jianli acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction AT pengchengfu acclimationandcharacterizationofmarinecyanobacterialstrainseuryhalinemaanddesertifilumforcphycocyaninproduction
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Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production

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