A combined approach for single-cell mRNA and intracellular protein expression analysis

Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor p...

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Autores principales: Johan Reimegård, Marcel Tarbier, Marcus Danielsson, Jens Schuster, Sathishkumar Baskaran, Styliani Panagiotou, Niklas Dahl, Marc R. Friedländer, Caroline J. Gallant
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b4
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spelling oai:doaj.org-article:f42cc4fcda564a3198a2a80c8aee12b42021-12-02T16:53:18ZA combined approach for single-cell mRNA and intracellular protein expression analysis10.1038/s42003-021-02142-w2399-3642https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b42021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02142-whttps://doaj.org/toc/2399-3642Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary.Johan ReimegårdMarcel TarbierMarcus DanielssonJens SchusterSathishkumar BaskaranStyliani PanagiotouNiklas DahlMarc R. FriedländerCaroline J. GallantNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Johan Reimegård
Marcel Tarbier
Marcus Danielsson
Jens Schuster
Sathishkumar Baskaran
Styliani Panagiotou
Niklas Dahl
Marc R. Friedländer
Caroline J. Gallant
A combined approach for single-cell mRNA and intracellular protein expression analysis
description Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary.
format article
author Johan Reimegård
Marcel Tarbier
Marcus Danielsson
Jens Schuster
Sathishkumar Baskaran
Styliani Panagiotou
Niklas Dahl
Marc R. Friedländer
Caroline J. Gallant
author_facet Johan Reimegård
Marcel Tarbier
Marcus Danielsson
Jens Schuster
Sathishkumar Baskaran
Styliani Panagiotou
Niklas Dahl
Marc R. Friedländer
Caroline J. Gallant
author_sort Johan Reimegård
title A combined approach for single-cell mRNA and intracellular protein expression analysis
title_short A combined approach for single-cell mRNA and intracellular protein expression analysis
title_full A combined approach for single-cell mRNA and intracellular protein expression analysis
title_fullStr A combined approach for single-cell mRNA and intracellular protein expression analysis
title_full_unstemmed A combined approach for single-cell mRNA and intracellular protein expression analysis
title_sort combined approach for single-cell mrna and intracellular protein expression analysis
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b4
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