A combined approach for single-cell mRNA and intracellular protein expression analysis

Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor p...

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Auteurs principaux:	Johan Reimegård, Marcel Tarbier, Marcus Danielsson, Jens Schuster, Sathishkumar Baskaran, Styliani Panagiotou, Niklas Dahl, Marc R. Friedländer, Caroline J. Gallant
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b4
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spelling	oai:doaj.org-article:f42cc4fcda564a3198a2a80c8aee12b42021-12-02T16:53:18ZA combined approach for single-cell mRNA and intracellular protein expression analysis10.1038/s42003-021-02142-w2399-3642https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b42021-05-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02142-whttps://doaj.org/toc/2399-3642Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary.Johan ReimegårdMarcel TarbierMarcus DanielssonJens SchusterSathishkumar BaskaranStyliani PanagiotouNiklas DahlMarc R. FriedländerCaroline J. GallantNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant A combined approach for single-cell mRNA and intracellular protein expression analysis
description	Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary.
format	article
author	Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant
author_facet	Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant
author_sort	Johan Reimegård
title	A combined approach for single-cell mRNA and intracellular protein expression analysis
title_short	A combined approach for single-cell mRNA and intracellular protein expression analysis
title_full	A combined approach for single-cell mRNA and intracellular protein expression analysis
title_fullStr	A combined approach for single-cell mRNA and intracellular protein expression analysis
title_full_unstemmed	A combined approach for single-cell mRNA and intracellular protein expression analysis
title_sort	combined approach for single-cell mrna and intracellular protein expression analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f42cc4fcda564a3198a2a80c8aee12b4
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A combined approach for single-cell mRNA and intracellular protein expression analysis

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