Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles

Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles

Sun-Ho Han1, Yu Jin Chang1, Eun Sun Jung1, Jong-Won Kim2, Duk Lyul Na3, Inhee Mook-Jung11Department of Biochemistry and Biomedical Sciences, Seoul National University, College of Medicine, Jongro-gu, Seoul, Korea; 2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Sun-Ho Han, Yu Jin Chang, Eun Sun Jung, et al
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2010
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/f42e036c07cc4ec08aff4bb7c4050c40
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:f42e036c07cc4ec08aff4bb7c4050c40
record_format dspace
spelling oai:doaj.org-article:f42e036c07cc4ec08aff4bb7c4050c402021-12-02T01:04:05ZEffective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles1176-91141178-2013https://doaj.org/article/f42e036c07cc4ec08aff4bb7c4050c402010-12-01T00:00:00Zhttp://www.dovepress.com/effective-screen-for-amyloid-beta-aggregation-inhibitor-using-amyloid--a5907https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sun-Ho Han1, Yu Jin Chang1, Eun Sun Jung1, Jong-Won Kim2, Duk Lyul Na3, Inhee Mook-Jung11Department of Biochemistry and Biomedical Sciences, Seoul National University, College of Medicine, Jongro-gu, Seoul, Korea; 2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Kangnam-Ku, Seoul, Korea; 3Department of Neurology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Kangnam-Ku, Seoul, KoreaAbstract: The abnormal aggregation of amyloid β (Aβ) and its subsequent intra- and extracellular accumulation constitute the disease-causing cascade of Alzheimer's disease (AD). The detection of Aβ aggregates and senile plaque formation, however, is nearly impossible during early pathogenesis, and the absence of a convenient screen to validate the activity of Aβ aggregation regulators impedes the development of promising drug targets and diagnostic biomarkers for AD. Here, we conjugated amyloid β42 (Aβ42) peptide to gold nanoparticles (AuNPs) to visualize Aβ42 aggregation via Aβ42 aggregation-induced AuNP precipitation. AuNP–Aβ42 precipitate was quantified by optical density measurements of supernatants and thioflavin T binding assay. Transmission electron microscopy (TEM) analysis also showed reduced interparticle distance of AuNPs and confirmed the Aβ42 aggregation-induced AuNP precipitation. Transthyretin, a widely known Aβ aggregation inhibitor, limited AuNP–Aβ42 precipitation by preventing Aβ42 aggregation. Finally, according to TEM analysis, Aβ42-conjugated AuNPs treated with blood-driven serum revealed the differentiated aggregation patterns between normal and AD. These findings may open a scientific breakthrough in finding a possible diagnostic and prognostic tool for neurodegenerative diseases involving abnormal protein aggregation as their key pathogenesis processes.Keywords: transthyretin, Alzheimer's disease, diagnosis, amyloid β aggregation, gold nanoparticle Sun-Ho HanYu Jin ChangEun Sun Junget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1-12 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Sun-Ho Han
Yu Jin Chang
Eun Sun Jung
et al
Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
description Sun-Ho Han1, Yu Jin Chang1, Eun Sun Jung1, Jong-Won Kim2, Duk Lyul Na3, Inhee Mook-Jung11Department of Biochemistry and Biomedical Sciences, Seoul National University, College of Medicine, Jongro-gu, Seoul, Korea; 2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Kangnam-Ku, Seoul, Korea; 3Department of Neurology, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Kangnam-Ku, Seoul, KoreaAbstract: The abnormal aggregation of amyloid β (Aβ) and its subsequent intra- and extracellular accumulation constitute the disease-causing cascade of Alzheimer's disease (AD). The detection of Aβ aggregates and senile plaque formation, however, is nearly impossible during early pathogenesis, and the absence of a convenient screen to validate the activity of Aβ aggregation regulators impedes the development of promising drug targets and diagnostic biomarkers for AD. Here, we conjugated amyloid β42 (Aβ42) peptide to gold nanoparticles (AuNPs) to visualize Aβ42 aggregation via Aβ42 aggregation-induced AuNP precipitation. AuNP–Aβ42 precipitate was quantified by optical density measurements of supernatants and thioflavin T binding assay. Transmission electron microscopy (TEM) analysis also showed reduced interparticle distance of AuNPs and confirmed the Aβ42 aggregation-induced AuNP precipitation. Transthyretin, a widely known Aβ aggregation inhibitor, limited AuNP–Aβ42 precipitation by preventing Aβ42 aggregation. Finally, according to TEM analysis, Aβ42-conjugated AuNPs treated with blood-driven serum revealed the differentiated aggregation patterns between normal and AD. These findings may open a scientific breakthrough in finding a possible diagnostic and prognostic tool for neurodegenerative diseases involving abnormal protein aggregation as their key pathogenesis processes.Keywords: transthyretin, Alzheimer's disease, diagnosis, amyloid β aggregation, gold nanoparticle
format article
author Sun-Ho Han
Yu Jin Chang
Eun Sun Jung
et al
author_facet Sun-Ho Han
Yu Jin Chang
Eun Sun Jung
et al
author_sort Sun-Ho Han
title Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
title_short Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
title_full Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
title_fullStr Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
title_full_unstemmed Effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
title_sort effective screen for amyloid β aggregation inhibitor using amyloid β-conjugated gold nanoparticles
publisher Dove Medical Press
publishDate 2010
url https://doaj.org/article/f42e036c07cc4ec08aff4bb7c4050c40
work_keys_str_mv AT sunhohan effectivescreenforamyloidampbetaaggregationinhibitorusingamyloidampbetaconjugatedgoldnanoparticles
AT yujinchang effectivescreenforamyloidampbetaaggregationinhibitorusingamyloidampbetaconjugatedgoldnanoparticles
AT eunsunjung effectivescreenforamyloidampbetaaggregationinhibitorusingamyloidampbetaconjugatedgoldnanoparticles
AT etal effectivescreenforamyloidampbetaaggregationinhibitorusingamyloidampbetaconjugatedgoldnanoparticles
_version_ 1718403353368592384

Ejemplares similares