Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo

Background: Maclura pomifera a member of Moraceae family, is a tropical plant with ethnobotanical uses. Objectives: The present study aimed to evaluate bioactive compounds, antioxidants, and acetylcholinesterase (AChE) inhibitory activities of different extracts of Tunisia M. pomifera (Rafin.) Sc...

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Autores principales:	Moahamed Mendili, Mohamed Ali Smach, Nedaa Husein, Ayda Khadhri
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2020
Materias:	maclura pomifera phenolic content antioxidant activity acetylcholinesterase inhibition in vivo in vitro Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/f42f254f5cca467888568a6a2ae1f966
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spelling	oai:doaj.org-article:f42f254f5cca467888568a6a2ae1f9662021-11-21T10:18:17ZComparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo10.34172/ajmb.2020.102345-4113https://doaj.org/article/f42f254f5cca467888568a6a2ae1f9662020-12-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-8-64.pdfhttps://doaj.org/toc/2345-4113Background: Maclura pomifera a member of Moraceae family, is a tropical plant with ethnobotanical uses. Objectives: The present study aimed to evaluate bioactive compounds, antioxidants, and acetylcholinesterase (AChE) inhibitory activities of different extracts of Tunisia M. pomifera (Rafin.) Schneid fruit in vitro and in vivo. Methods: Organic extracts in different polarities (chloroform, ethyl acetate, and acetone) were extracted from different parts of the fruit of M. pomifera (exocarp, mesocarp, and pips). Phenolic content, antioxidant activity, and anti-AChE activity were determined. The anti-amnesic effects of ethyl acetate extract of the exocarp of M. pomifera were measured in galactose-induced memory deficit mice by the Y maze. The levels of biomarkers and AChE activity were determined in brain tissues. Results: The obtained results showed that the ethyl acetate extract of exocarp contains the highest content of flavonoids and polyphenols 22.3mg quercetin equivalents per g of dry weight and 718.6 mg gallic acid equivalents per g of dry weight. The evaluation of antioxidant activities highlighted that the ethyl acetate extract of exocarp was the most active element. The study of the AChE inhibitory power demonstrated that the ethyl acetate extract of the exocarp had the greatest inhibitory activity. The ethyl acetate extract from the exocarp ameliorated cognitive performance and reversed the oxidative damage as compared to galactose group. Conclusion: M. pomifera fruit is a good source of natural antioxidants, which might help prevent oxidative stress-related damage and memory impairment in such mental disorders as Alzheimer’s disease (AD).Moahamed MendiliMohamed Ali SmachNedaa HuseinAyda KhadhriHamadan University of Medical Sciencesarticlemaclura pomiferaphenolic contentantioxidant activityacetylcholinesterase inhibitionin vivoin vitroMedical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 8, Iss 2, Pp 64-73 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	maclura pomifera phenolic content antioxidant activity acetylcholinesterase inhibition in vivo in vitro Medical technology R855-855.5
spellingShingle	maclura pomifera phenolic content antioxidant activity acetylcholinesterase inhibition in vivo in vitro Medical technology R855-855.5 Moahamed Mendili Mohamed Ali Smach Nedaa Husein Ayda Khadhri Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
description	Background: Maclura pomifera a member of Moraceae family, is a tropical plant with ethnobotanical uses. Objectives: The present study aimed to evaluate bioactive compounds, antioxidants, and acetylcholinesterase (AChE) inhibitory activities of different extracts of Tunisia M. pomifera (Rafin.) Schneid fruit in vitro and in vivo. Methods: Organic extracts in different polarities (chloroform, ethyl acetate, and acetone) were extracted from different parts of the fruit of M. pomifera (exocarp, mesocarp, and pips). Phenolic content, antioxidant activity, and anti-AChE activity were determined. The anti-amnesic effects of ethyl acetate extract of the exocarp of M. pomifera were measured in galactose-induced memory deficit mice by the Y maze. The levels of biomarkers and AChE activity were determined in brain tissues. Results: The obtained results showed that the ethyl acetate extract of exocarp contains the highest content of flavonoids and polyphenols 22.3mg quercetin equivalents per g of dry weight and 718.6 mg gallic acid equivalents per g of dry weight. The evaluation of antioxidant activities highlighted that the ethyl acetate extract of exocarp was the most active element. The study of the AChE inhibitory power demonstrated that the ethyl acetate extract of the exocarp had the greatest inhibitory activity. The ethyl acetate extract from the exocarp ameliorated cognitive performance and reversed the oxidative damage as compared to galactose group. Conclusion: M. pomifera fruit is a good source of natural antioxidants, which might help prevent oxidative stress-related damage and memory impairment in such mental disorders as Alzheimer’s disease (AD).
format	article
author	Moahamed Mendili Mohamed Ali Smach Nedaa Husein Ayda Khadhri
author_facet	Moahamed Mendili Mohamed Ali Smach Nedaa Husein Ayda Khadhri
author_sort	Moahamed Mendili
title	Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
title_short	Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
title_full	Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
title_fullStr	Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
title_full_unstemmed	Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo
title_sort	comparison of antioxidant and antiacetylcholinesterase activities of different extracts of tunisia maclura pomifera (rafin.) schneid fruit in vitro and in vivo
publisher	Hamadan University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/f42f254f5cca467888568a6a2ae1f966
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Comparison of Antioxidant and Antiacetylcholinesterase Activities of Different Extracts of Tunisia Maclura pomifera (Rafin.) Schneid Fruit In Vitro and In Vivo

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