Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties

Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties

The aim of this study was to optimize the extraction method for caffeic acid from the leaves of <i>Dendropanax morbifera</i> using response surface methodology (RSM), and subsequently determine the polyphenolic content and antioxidant capacity of the <i>D. morbifera</i> leave...

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Autores principales: Ming Zhang, Ting Bu, Shuilin Liu, Sooah Kim
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
antioxidant
caffeic acid
<i>Dendropanax morbifera</i>
extraction
polyphenols
response surface methodology
Plant culture
SB1-1110
Acceso en línea:https://doaj.org/article/f4328b53999644c0a3a5d1574ea0dead
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spelling oai:doaj.org-article:f4328b53999644c0a3a5d1574ea0dead2021-11-25T17:47:36ZOptimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties10.3390/horticulturae71104912311-7524https://doaj.org/article/f4328b53999644c0a3a5d1574ea0dead2021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/491https://doaj.org/toc/2311-7524The aim of this study was to optimize the extraction method for caffeic acid from the leaves of <i>Dendropanax morbifera</i> using response surface methodology (RSM), and subsequently determine the polyphenolic content and antioxidant capacity of the <i>D. morbifera</i> leaves extracts. The extraction process considered operating variables such as solvent concentration, extraction temperature, and extraction time, which were optimized via Box–Behnken experimental design. In addition, the antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The optimal extraction conditions of 41.23% (<i>v/v</i>) methanol concentration, at 88.61 °C, and 1.86 h produced a maximum caffeic acid (CA) yield of 20.35 mg/g. Additionally, total polyphenols, total tannins, and total flavonoids contents in the leaves extracts of <i>D. morbifera</i> were 32.48 ± 2.05 mg GAE (gallic acid equivalents)/g, 17.61 ± 2.61 mg GAE/g, and 9.14 ± 0.17 mg QE (quercetin equivalents)/g, respectively. The results showed that methanol extracts exhibited an IC50 of approximately 14.3 mg AAE (ascorbic acid equivalent)/g. The results suggested that the extracts of <i>D. morbifera</i> leaves contain potential antioxidant activity, and could be a good source of functional food and used in medicinal applications.Ming ZhangTing BuShuilin LiuSooah KimMDPI AGarticleantioxidantcaffeic acid<i>Dendropanax morbifera</i>extractionpolyphenolsresponse surface methodologyPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 491, p 491 (2021)
institution DOAJ
collection DOAJ
language EN
topic antioxidant
caffeic acid
<i>Dendropanax morbifera</i>
extraction
polyphenols
response surface methodology
Plant culture
SB1-1110
spellingShingle antioxidant
caffeic acid
<i>Dendropanax morbifera</i>
extraction
polyphenols
response surface methodology
Plant culture
SB1-1110
Ming Zhang
Ting Bu
Shuilin Liu
Sooah Kim
Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
description The aim of this study was to optimize the extraction method for caffeic acid from the leaves of <i>Dendropanax morbifera</i> using response surface methodology (RSM), and subsequently determine the polyphenolic content and antioxidant capacity of the <i>D. morbifera</i> leaves extracts. The extraction process considered operating variables such as solvent concentration, extraction temperature, and extraction time, which were optimized via Box–Behnken experimental design. In addition, the antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The optimal extraction conditions of 41.23% (<i>v/v</i>) methanol concentration, at 88.61 °C, and 1.86 h produced a maximum caffeic acid (CA) yield of 20.35 mg/g. Additionally, total polyphenols, total tannins, and total flavonoids contents in the leaves extracts of <i>D. morbifera</i> were 32.48 ± 2.05 mg GAE (gallic acid equivalents)/g, 17.61 ± 2.61 mg GAE/g, and 9.14 ± 0.17 mg QE (quercetin equivalents)/g, respectively. The results showed that methanol extracts exhibited an IC50 of approximately 14.3 mg AAE (ascorbic acid equivalent)/g. The results suggested that the extracts of <i>D. morbifera</i> leaves contain potential antioxidant activity, and could be a good source of functional food and used in medicinal applications.
format article
author Ming Zhang
Ting Bu
Shuilin Liu
Sooah Kim
author_facet Ming Zhang
Ting Bu
Shuilin Liu
Sooah Kim
author_sort Ming Zhang
title Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
title_short Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
title_full Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
title_fullStr Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
title_full_unstemmed Optimization of Caffeic Acid Extraction from <i>Dendropanax morbifera</i> Leaves Using Response Surface Methodology and Determination of Polyphenols and Antioxidant Properties
title_sort optimization of caffeic acid extraction from <i>dendropanax morbifera</i> leaves using response surface methodology and determination of polyphenols and antioxidant properties
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/f4328b53999644c0a3a5d1574ea0dead
work_keys_str_mv AT mingzhang optimizationofcaffeicacidextractionfromidendropanaxmorbiferaileavesusingresponsesurfacemethodologyanddeterminationofpolyphenolsandantioxidantproperties
AT tingbu optimizationofcaffeicacidextractionfromidendropanaxmorbiferaileavesusingresponsesurfacemethodologyanddeterminationofpolyphenolsandantioxidantproperties
AT shuilinliu optimizationofcaffeicacidextractionfromidendropanaxmorbiferaileavesusingresponsesurfacemethodologyanddeterminationofpolyphenolsandantioxidantproperties
AT sooahkim optimizationofcaffeicacidextractionfromidendropanaxmorbiferaileavesusingresponsesurfacemethodologyanddeterminationofpolyphenolsandantioxidantproperties
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