Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry

Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was use...

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Autores principales:	Lucia Borszéková Pulzová, Jan Roška, Michal Kalman, Ján Kliment, Pavol Slávik, Božena Smolková, Eduard Goffa, Dana Jurkovičová, Ľudovít Kulcsár, Katarína Lešková, Peter Bujdák, Michal Mego, Mangesh R. Bhide, Lukáš Plank, Miroslav Chovanec
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	testicular germ cell tumours clinical stage I seminoma rete testis invasion proteomics mesenchymal type proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/f434874ffa024723896a3e8b32349887
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spelling	oai:doaj.org-article:f434874ffa024723896a3e8b323498872021-11-11T15:35:42ZScreening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry10.3390/cancers132155732072-6694https://doaj.org/article/f434874ffa024723896a3e8b323498872021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5573https://doaj.org/toc/2072-6694Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was used to investigate RTI-associated proteins. 64 proteins were differentially expressed in RTI-positive compared to -negative CS I seminomas. Of them, 14-3-3γ, ezrin, filamin A, Parkinsonism-associated deglycase 7 (PARK7), vimentin and vinculin, were validated in CS I seminoma patient cohort. As shown by multivariate analysis controlling for clinical confounders, PARK7 and filamin A expression lowered the risk of RTI, while 14-3-3γ expression increased it. Therefore, we suggest that in real clinical biopsy specimens, the expression level of these proteins may reflect prognosis in CS I seminoma patients.Lucia Borszéková PulzováJan RoškaMichal KalmanJán KlimentPavol SlávikBožena SmolkováEduard GoffaDana JurkovičováĽudovít KulcsárKatarína LeškováPeter BujdákMichal MegoMangesh R. BhideLukáš PlankMiroslav ChovanecMDPI AGarticletesticular germ cell tumoursclinical stage I seminomarete testis invasionproteomicsmesenchymal type proteinsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5573, p 5573 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	testicular germ cell tumours clinical stage I seminoma rete testis invasion proteomics mesenchymal type proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	testicular germ cell tumours clinical stage I seminoma rete testis invasion proteomics mesenchymal type proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Lucia Borszéková Pulzová Jan Roška Michal Kalman Ján Kliment Pavol Slávik Božena Smolková Eduard Goffa Dana Jurkovičová Ľudovít Kulcsár Katarína Lešková Peter Bujdák Michal Mego Mangesh R. Bhide Lukáš Plank Miroslav Chovanec Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
description	Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was used to investigate RTI-associated proteins. 64 proteins were differentially expressed in RTI-positive compared to -negative CS I seminomas. Of them, 14-3-3γ, ezrin, filamin A, Parkinsonism-associated deglycase 7 (PARK7), vimentin and vinculin, were validated in CS I seminoma patient cohort. As shown by multivariate analysis controlling for clinical confounders, PARK7 and filamin A expression lowered the risk of RTI, while 14-3-3γ expression increased it. Therefore, we suggest that in real clinical biopsy specimens, the expression level of these proteins may reflect prognosis in CS I seminoma patients.
format	article
author	Lucia Borszéková Pulzová Jan Roška Michal Kalman Ján Kliment Pavol Slávik Božena Smolková Eduard Goffa Dana Jurkovičová Ľudovít Kulcsár Katarína Lešková Peter Bujdák Michal Mego Mangesh R. Bhide Lukáš Plank Miroslav Chovanec
author_facet	Lucia Borszéková Pulzová Jan Roška Michal Kalman Ján Kliment Pavol Slávik Božena Smolková Eduard Goffa Dana Jurkovičová Ľudovít Kulcsár Katarína Lešková Peter Bujdák Michal Mego Mangesh R. Bhide Lukáš Plank Miroslav Chovanec
author_sort	Lucia Borszéková Pulzová
title	Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
title_short	Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
title_full	Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
title_fullStr	Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
title_full_unstemmed	Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry
title_sort	screening for the key proteins associated with rete testis invasion in clinical stage i seminoma via label-free quantitative mass spectrometry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f434874ffa024723896a3e8b32349887
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Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry

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