Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells

Abstract Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and...

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Autores principales:	Ulf Hedström, Oskar Hallgren, Lisa Öberg, Amy DeMicco, Outi Vaarala, Gunilla Westergren-Thorsson, Xiaohong Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f43b5198fe3f450ba3479b461c43074f
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spelling	oai:doaj.org-article:f43b5198fe3f450ba3479b461c43074f2021-12-02T11:40:36ZBronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells10.1038/s41598-018-21727-w2045-2322https://doaj.org/article/f43b5198fe3f450ba3479b461c43074f2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21727-whttps://doaj.org/toc/2045-2322Abstract Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals. By using transcriptomics, we show that bronchial ECM from COPD patients induces differential gene expression in primary NHBE cells when compared to normal bronchial ECM. The gene expression profile indicated altered activity of upstream mediators associated with COPD pathophysiology, including hepatocyte growth factor, transforming growth factor beta 1 and platelet-derived growth factor B, which suggests that COPD-related changes in the bronchial ECM contribute to the defective regenerative ability in the airways of COPD patients.Ulf HedströmOskar HallgrenLisa ÖbergAmy DeMiccoOuti VaaralaGunilla Westergren-ThorssonXiaohong ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulf Hedström Oskar Hallgren Lisa Öberg Amy DeMicco Outi Vaarala Gunilla Westergren-Thorsson Xiaohong Zhou Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
description	Abstract Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals. By using transcriptomics, we show that bronchial ECM from COPD patients induces differential gene expression in primary NHBE cells when compared to normal bronchial ECM. The gene expression profile indicated altered activity of upstream mediators associated with COPD pathophysiology, including hepatocyte growth factor, transforming growth factor beta 1 and platelet-derived growth factor B, which suggests that COPD-related changes in the bronchial ECM contribute to the defective regenerative ability in the airways of COPD patients.
format	article
author	Ulf Hedström Oskar Hallgren Lisa Öberg Amy DeMicco Outi Vaarala Gunilla Westergren-Thorsson Xiaohong Zhou
author_facet	Ulf Hedström Oskar Hallgren Lisa Öberg Amy DeMicco Outi Vaarala Gunilla Westergren-Thorsson Xiaohong Zhou
author_sort	Ulf Hedström
title	Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
title_short	Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
title_full	Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
title_fullStr	Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
title_full_unstemmed	Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
title_sort	bronchial extracellular matrix from copd patients induces altered gene expression in repopulated primary human bronchial epithelial cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f43b5198fe3f450ba3479b461c43074f
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_version_	1718395618253078528

Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells

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