Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens

Abstract Phosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. Ther...

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Autores principales:	Muhammad Arsalan Iqbal, Asghar Ali, Frieder Hadlich, Michael Oster, Henry Reyer, Nares Trakooljul, Vera Sommerfeld, Markus Rodehutscord, Klaus Wimmers, Siriluck Ponsuksili
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f43cf73926264b05acf2341b37c2c990
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spelling	oai:doaj.org-article:f43cf73926264b05acf2341b37c2c9902021-12-02T18:18:51ZDietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens10.1038/s41598-021-92932-32045-2322https://doaj.org/article/f43cf73926264b05acf2341b37c2c9902021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92932-3https://doaj.org/toc/2045-2322Abstract Phosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (n = 80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.Muhammad Arsalan IqbalAsghar AliFrieder HadlichMichael OsterHenry ReyerNares TrakooljulVera SommerfeldMarkus RodehutscordKlaus WimmersSiriluck PonsuksiliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Arsalan Iqbal Asghar Ali Frieder Hadlich Michael Oster Henry Reyer Nares Trakooljul Vera Sommerfeld Markus Rodehutscord Klaus Wimmers Siriluck Ponsuksili Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
description	Abstract Phosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (n = 80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.
format	article
author	Muhammad Arsalan Iqbal Asghar Ali Frieder Hadlich Michael Oster Henry Reyer Nares Trakooljul Vera Sommerfeld Markus Rodehutscord Klaus Wimmers Siriluck Ponsuksili
author_facet	Muhammad Arsalan Iqbal Asghar Ali Frieder Hadlich Michael Oster Henry Reyer Nares Trakooljul Vera Sommerfeld Markus Rodehutscord Klaus Wimmers Siriluck Ponsuksili
author_sort	Muhammad Arsalan Iqbal
title	Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
title_short	Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
title_full	Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
title_fullStr	Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
title_full_unstemmed	Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens
title_sort	dietary phosphorus and calcium in feed affects mirna profiles and their mrna targets in jejunum of two strains of laying hens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f43cf73926264b05acf2341b37c2c990
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Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens

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