Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia
The aim of this preliminary study was to investigate the presence of <i>Mycoplasma agalactiae</i> (<i>Ma</i>) or other Contagious Agalactia (CA) causative organisms, in hard ticks infesting milking sheep and goats in endemic areas for CA in Sicily (South-Italy). Although ther...
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2021
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oai:doaj.org-article:f443fbda811c4873b401c1355490d8272021-11-25T18:25:02ZDetection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia10.3390/microorganisms91123122076-2607https://doaj.org/article/f443fbda811c4873b401c1355490d8272021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2312https://doaj.org/toc/2076-2607The aim of this preliminary study was to investigate the presence of <i>Mycoplasma agalactiae</i> (<i>Ma</i>) or other Contagious Agalactia (CA) causative organisms, in hard ticks infesting milking sheep and goats in endemic areas for CA in Sicily (South-Italy). Although there is accumulating evidence to support the role of ticks in the transmission of blood-borne haemoplasmas, information regarding their role in the transmission of CA, remains scarce. Ticks (<i>n</i> = 152) were collected from 25 lactating sheep and goats from three farms with previous outbreaks of CA. Microbiological and biomolecular, as well as serological analysis were performed on milk, tick, and serum samples, respectively. <i>Rhipicephalus bursa</i> species predominated, comprising 84.8% of the sampled ticks. Mycoplasma-like colonies were isolated from 5/56 (8.9%) tick pools and were identified as <i>Ma</i> by specific PCR and 16S rRNA gene sequencing. Unexpectedly, the organism was isolated from <i>R. bursa</i> ticks recovered only from animals whose milk tested negative for the pathogen. This preliminary demonstration suggests the potential role for ticks to act as a reservoir for the organisms, with potential involvement in the spread and maintenance of CA. Further work is required to determine the location of the organisms within the body of the ticks and to assess transmission potential.Paola GalluzzoSergio MiglioreRoberto PuleioLucia GaluppoFrancesco La RussaValeria BlandaSerena TuminoAlessandra TorinaAnne RidleyGuido R. LoriaMDPI AGarticlecontagious agalactia<i>Mycoplasma agalactiae</i>tickssheep and goatsBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2312, p 2312 (2021) |
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contagious agalactia <i>Mycoplasma agalactiae</i> ticks sheep and goats Biology (General) QH301-705.5 |
| spellingShingle |
contagious agalactia <i>Mycoplasma agalactiae</i> ticks sheep and goats Biology (General) QH301-705.5 Paola Galluzzo Sergio Migliore Roberto Puleio Lucia Galuppo Francesco La Russa Valeria Blanda Serena Tumino Alessandra Torina Anne Ridley Guido R. Loria Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| description |
The aim of this preliminary study was to investigate the presence of <i>Mycoplasma agalactiae</i> (<i>Ma</i>) or other Contagious Agalactia (CA) causative organisms, in hard ticks infesting milking sheep and goats in endemic areas for CA in Sicily (South-Italy). Although there is accumulating evidence to support the role of ticks in the transmission of blood-borne haemoplasmas, information regarding their role in the transmission of CA, remains scarce. Ticks (<i>n</i> = 152) were collected from 25 lactating sheep and goats from three farms with previous outbreaks of CA. Microbiological and biomolecular, as well as serological analysis were performed on milk, tick, and serum samples, respectively. <i>Rhipicephalus bursa</i> species predominated, comprising 84.8% of the sampled ticks. Mycoplasma-like colonies were isolated from 5/56 (8.9%) tick pools and were identified as <i>Ma</i> by specific PCR and 16S rRNA gene sequencing. Unexpectedly, the organism was isolated from <i>R. bursa</i> ticks recovered only from animals whose milk tested negative for the pathogen. This preliminary demonstration suggests the potential role for ticks to act as a reservoir for the organisms, with potential involvement in the spread and maintenance of CA. Further work is required to determine the location of the organisms within the body of the ticks and to assess transmission potential. |
| format |
article |
| author |
Paola Galluzzo Sergio Migliore Roberto Puleio Lucia Galuppo Francesco La Russa Valeria Blanda Serena Tumino Alessandra Torina Anne Ridley Guido R. Loria |
| author_facet |
Paola Galluzzo Sergio Migliore Roberto Puleio Lucia Galuppo Francesco La Russa Valeria Blanda Serena Tumino Alessandra Torina Anne Ridley Guido R. Loria |
| author_sort |
Paola Galluzzo |
| title |
Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| title_short |
Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| title_full |
Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| title_fullStr |
Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| title_full_unstemmed |
Detection of <i>Mycoplasma agalactiae</i> in Ticks (<i>Rhipicephalus bursa</i>) Collected by Sheep and Goats in Sicily (South-Italy), Endemic Area for Contagious Agalactia |
| title_sort |
detection of <i>mycoplasma agalactiae</i> in ticks (<i>rhipicephalus bursa</i>) collected by sheep and goats in sicily (south-italy), endemic area for contagious agalactia |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/f443fbda811c4873b401c1355490d827 |
| work_keys_str_mv |
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| _version_ |
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