Functional conservation and coherence of HIV-1 subtype A Vpu alleles

Abstract Functional studies of HIV-1 proteins are normally conducted using lab adapted strains of HIV-1. The extent of those functions in clinical strains is sometimes unknown. In this study, we amplified and sequenced HIV-1 Vpu from 10 Iranian patients infected with HIV-1. Phylogenetic analysis ind...

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Autores principales:	Bizhan Romani, Amirarsalan Kavyanifard, Elham Allahbakhshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f44bc48575bb4309b46f11a33edb0ffa
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spelling	oai:doaj.org-article:f44bc48575bb4309b46f11a33edb0ffa2021-12-02T15:05:55ZFunctional conservation and coherence of HIV-1 subtype A Vpu alleles10.1038/s41598-017-00222-82045-2322https://doaj.org/article/f44bc48575bb4309b46f11a33edb0ffa2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00222-8https://doaj.org/toc/2045-2322Abstract Functional studies of HIV-1 proteins are normally conducted using lab adapted strains of HIV-1. The extent of those functions in clinical strains is sometimes unknown. In this study, we amplified and sequenced HIV-1 Vpu from 10 Iranian patients infected with HIV-1. Phylogenetic analysis indicated that the Vpu alleles were closely related to the CRF35_AD from Iran and subtype A Vpu. We addressed some of the well-established functions of the HIV-1 Vpu, as well as some of its recently reported functions. Ability of the clinical strains of subtype A Vpu alleles for downregulation of CD4 was similar to that of the lab adapted NL4.3 Vpu. Majority of the subtype A Vpu alleles performed stronger than NL4.3 Vpu for downregulation of SNAT1. The Vpu alleles differentially induced downregulation of HLA-C, ranging from no effect to 88% downregulation of surface HLA-C. Downregulation of tetherin and enhancement of virus release was similar for the subtype A Vpu alleles and NL4.3. Subtype A Vpu alleles were more potent when compared with NL4.3 for inhibition of NF-κB activation. Our study shows that subtype A Vpu alleles exert the classical functions of HIV-1 Vpu.Bizhan RomaniAmirarsalan KavyanifardElham AllahbakhshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bizhan Romani Amirarsalan Kavyanifard Elham Allahbakhshi Functional conservation and coherence of HIV-1 subtype A Vpu alleles
description	Abstract Functional studies of HIV-1 proteins are normally conducted using lab adapted strains of HIV-1. The extent of those functions in clinical strains is sometimes unknown. In this study, we amplified and sequenced HIV-1 Vpu from 10 Iranian patients infected with HIV-1. Phylogenetic analysis indicated that the Vpu alleles were closely related to the CRF35_AD from Iran and subtype A Vpu. We addressed some of the well-established functions of the HIV-1 Vpu, as well as some of its recently reported functions. Ability of the clinical strains of subtype A Vpu alleles for downregulation of CD4 was similar to that of the lab adapted NL4.3 Vpu. Majority of the subtype A Vpu alleles performed stronger than NL4.3 Vpu for downregulation of SNAT1. The Vpu alleles differentially induced downregulation of HLA-C, ranging from no effect to 88% downregulation of surface HLA-C. Downregulation of tetherin and enhancement of virus release was similar for the subtype A Vpu alleles and NL4.3. Subtype A Vpu alleles were more potent when compared with NL4.3 for inhibition of NF-κB activation. Our study shows that subtype A Vpu alleles exert the classical functions of HIV-1 Vpu.
format	article
author	Bizhan Romani Amirarsalan Kavyanifard Elham Allahbakhshi
author_facet	Bizhan Romani Amirarsalan Kavyanifard Elham Allahbakhshi
author_sort	Bizhan Romani
title	Functional conservation and coherence of HIV-1 subtype A Vpu alleles
title_short	Functional conservation and coherence of HIV-1 subtype A Vpu alleles
title_full	Functional conservation and coherence of HIV-1 subtype A Vpu alleles
title_fullStr	Functional conservation and coherence of HIV-1 subtype A Vpu alleles
title_full_unstemmed	Functional conservation and coherence of HIV-1 subtype A Vpu alleles
title_sort	functional conservation and coherence of hiv-1 subtype a vpu alleles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f44bc48575bb4309b46f11a33edb0ffa
work_keys_str_mv	AT bizhanromani functionalconservationandcoherenceofhiv1subtypeavpualleles AT amirarsalankavyanifard functionalconservationandcoherenceofhiv1subtypeavpualleles AT elhamallahbakhshi functionalconservationandcoherenceofhiv1subtypeavpualleles
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Functional conservation and coherence of HIV-1 subtype A Vpu alleles

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