Messenger RNAs localized to distal projections of human stem cell derived neurons

Abstract The identification of mRNAs in distal projections of model organisms has led to the discovery of multiple proteins that are locally synthesized for functional roles such as axon guidance, injury signaling and regeneration. The extent to which local protein synthesis is conserved in human ne...

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Autores principales:	Rebecca L. Bigler, Joyce W. Kamande, Raluca Dumitru, Mark Niedringhaus, Anne Marion Taylor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f44e3cec4ede4ee5a6138c9055d581d4
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spelling	oai:doaj.org-article:f44e3cec4ede4ee5a6138c9055d581d42021-12-02T11:52:39ZMessenger RNAs localized to distal projections of human stem cell derived neurons10.1038/s41598-017-00676-w2045-2322https://doaj.org/article/f44e3cec4ede4ee5a6138c9055d581d42017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00676-whttps://doaj.org/toc/2045-2322Abstract The identification of mRNAs in distal projections of model organisms has led to the discovery of multiple proteins that are locally synthesized for functional roles such as axon guidance, injury signaling and regeneration. The extent to which local protein synthesis is conserved in human neurons is unknown. Here we used compartmentalized microfluidic chambers to characterize the transcriptome of distal projections of human embryonic stem cells differentiated using a protocol which enriched for glutamatergic neurons (hESC-neurons). Using gene expression analysis, we identified mRNAs proportionally enriched in these projections, representing a functionally unique local transcriptome as compared to the human neuronal transcriptome inclusive of somata. Further, we found that the most abundant mRNAs within these hESC-neuron projections were functionally similar to the axonal transcriptome of rat cortical neurons. We confirmed the presence of two well characterized axonal mRNAs in model organisms, β-actin and GAP43, within hESC-neuron projections using multiplexed single molecule RNA-FISH. Additionally, we report the novel finding that oxytocin mRNA localized to these human projections and confirmed its localization using RNA-FISH. This new evaluation of mRNA within human projections provides an important resource for studying local mRNA translation and has the potential to reveal both conserved and unique translation dependent mechanisms.Rebecca L. BiglerJoyce W. KamandeRaluca DumitruMark NiedringhausAnne Marion TaylorNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebecca L. Bigler Joyce W. Kamande Raluca Dumitru Mark Niedringhaus Anne Marion Taylor Messenger RNAs localized to distal projections of human stem cell derived neurons
description	Abstract The identification of mRNAs in distal projections of model organisms has led to the discovery of multiple proteins that are locally synthesized for functional roles such as axon guidance, injury signaling and regeneration. The extent to which local protein synthesis is conserved in human neurons is unknown. Here we used compartmentalized microfluidic chambers to characterize the transcriptome of distal projections of human embryonic stem cells differentiated using a protocol which enriched for glutamatergic neurons (hESC-neurons). Using gene expression analysis, we identified mRNAs proportionally enriched in these projections, representing a functionally unique local transcriptome as compared to the human neuronal transcriptome inclusive of somata. Further, we found that the most abundant mRNAs within these hESC-neuron projections were functionally similar to the axonal transcriptome of rat cortical neurons. We confirmed the presence of two well characterized axonal mRNAs in model organisms, β-actin and GAP43, within hESC-neuron projections using multiplexed single molecule RNA-FISH. Additionally, we report the novel finding that oxytocin mRNA localized to these human projections and confirmed its localization using RNA-FISH. This new evaluation of mRNA within human projections provides an important resource for studying local mRNA translation and has the potential to reveal both conserved and unique translation dependent mechanisms.
format	article
author	Rebecca L. Bigler Joyce W. Kamande Raluca Dumitru Mark Niedringhaus Anne Marion Taylor
author_facet	Rebecca L. Bigler Joyce W. Kamande Raluca Dumitru Mark Niedringhaus Anne Marion Taylor
author_sort	Rebecca L. Bigler
title	Messenger RNAs localized to distal projections of human stem cell derived neurons
title_short	Messenger RNAs localized to distal projections of human stem cell derived neurons
title_full	Messenger RNAs localized to distal projections of human stem cell derived neurons
title_fullStr	Messenger RNAs localized to distal projections of human stem cell derived neurons
title_full_unstemmed	Messenger RNAs localized to distal projections of human stem cell derived neurons
title_sort	messenger rnas localized to distal projections of human stem cell derived neurons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f44e3cec4ede4ee5a6138c9055d581d4
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_version_	1718395021171884032

Messenger RNAs localized to distal projections of human stem cell derived neurons

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