IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers

Streptococcus pneumoniae are significant causative agents of severe and life-threatening acute pneumonia, meningitis, as well as otitis and sinusitis both in children and elderly. As many as 1.2 million pediatric lethal outcomes due to pneumonia and infections of the central nervous system (meningit...

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Autores principales:	A. Z. Zaripova, Yu. A. Tyurin, L. T. Bayazitova, O. F. Tyupkina, G. Sh. Isaeva
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	streptococcus pneumoniae serotypes iga protease activity carrier children Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f450bce224fa42b88f2d3e1af92a8662
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spelling	oai:doaj.org-article:f450bce224fa42b88f2d3e1af92a86622021-11-22T07:09:53ZIgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers2220-76192313-739810.15789/2220-7619-2019-5-6-680-686https://doaj.org/article/f450bce224fa42b88f2d3e1af92a86622020-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/693https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Streptococcus pneumoniae are significant causative agents of severe and life-threatening acute pneumonia, meningitis, as well as otitis and sinusitis both in children and elderly. As many as 1.2 million pediatric lethal outcomes due to pneumonia and infections of the central nervous system (meningitis) caused by S. pneumoniae, are recorded worldwide annually, a large proportion of which occur in developing countries. Metal-dependent IgA1 proteases derived from pathogenic bacteria comprise an important group of bacterial enzymes cleaving human immunoglobulin A1 (IgA1) at the hinge region, thereby interfering with fully-executed host antibacterial immunity.Objective. To study activity of IgA1proteinases and their class profile (Na2-EDTA and PMSF-inhibited) in various pneumococcal serotypes isolated from nasopharyngeal carrier children.Materials and methods. There were examined 585 children attending preschool facilities residing in Kazan (n = 331) and rural areas (n = 254). Microbiological, molecular genetics and immunochemical methods were used to identify, serotyping composition and protease activity of Streptococcus pneumoniae isolates. Data statistical processing was carried out by using software Graph Pad Prism version 5.0.Results. Prevalence of S. pneumonie in pediatric carriers aged 1.5–3 years was 35.1%, 3–5 years — 23.4%, 5–7 years — 19.6%, and over 7 years — 21.9%. Vaccine serotypes 14, 19F, 23F as a part of current pneumococcal vaccines (Prevenar, Pneumavax-23) comprised as high as 55.8%. However, in 19% of cases were positive for non-vaccine S. pneumoniae strains. Non-typeable strains were detected in 5.8% isolates. IgA-proteinase activity was detected in cell lysates of 45 (86.5%) S. pneumoniae strains isolated from pediatric carriers. Cell lysates of S. pneumoniae strains showing no proteolytic properties, were assigned to serotypes 12F, Sg18. Thus, studies on development of alternative vaccines containing immunogenic proteins, adhesins or other virulence factors common to capsulated and non-typeable (encapsulated) pneumococcal strains hold promise. All the aforementioned accounts for a need for microbiological monitoring of S. pneumoniae carriage and search for new diagnostic approaches for etiological interpretation of S. pneumoniae-associated diseases.A. Z. ZaripovaYu. A. TyurinL. T. BayazitovaO. F. TyupkinaG. Sh. IsaevaSankt-Peterburg : NIIÈM imeni Pasteraarticlestreptococcus pneumoniaeserotypesiga protease activitycarrierchildrenInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 5-6, Pp 680-686 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	streptococcus pneumoniae serotypes iga protease activity carrier children Infectious and parasitic diseases RC109-216
spellingShingle	streptococcus pneumoniae serotypes iga protease activity carrier children Infectious and parasitic diseases RC109-216 A. Z. Zaripova Yu. A. Tyurin L. T. Bayazitova O. F. Tyupkina G. Sh. Isaeva IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
description	Streptococcus pneumoniae are significant causative agents of severe and life-threatening acute pneumonia, meningitis, as well as otitis and sinusitis both in children and elderly. As many as 1.2 million pediatric lethal outcomes due to pneumonia and infections of the central nervous system (meningitis) caused by S. pneumoniae, are recorded worldwide annually, a large proportion of which occur in developing countries. Metal-dependent IgA1 proteases derived from pathogenic bacteria comprise an important group of bacterial enzymes cleaving human immunoglobulin A1 (IgA1) at the hinge region, thereby interfering with fully-executed host antibacterial immunity.Objective. To study activity of IgA1proteinases and their class profile (Na2-EDTA and PMSF-inhibited) in various pneumococcal serotypes isolated from nasopharyngeal carrier children.Materials and methods. There were examined 585 children attending preschool facilities residing in Kazan (n = 331) and rural areas (n = 254). Microbiological, molecular genetics and immunochemical methods were used to identify, serotyping composition and protease activity of Streptococcus pneumoniae isolates. Data statistical processing was carried out by using software Graph Pad Prism version 5.0.Results. Prevalence of S. pneumonie in pediatric carriers aged 1.5–3 years was 35.1%, 3–5 years — 23.4%, 5–7 years — 19.6%, and over 7 years — 21.9%. Vaccine serotypes 14, 19F, 23F as a part of current pneumococcal vaccines (Prevenar, Pneumavax-23) comprised as high as 55.8%. However, in 19% of cases were positive for non-vaccine S. pneumoniae strains. Non-typeable strains were detected in 5.8% isolates. IgA-proteinase activity was detected in cell lysates of 45 (86.5%) S. pneumoniae strains isolated from pediatric carriers. Cell lysates of S. pneumoniae strains showing no proteolytic properties, were assigned to serotypes 12F, Sg18. Thus, studies on development of alternative vaccines containing immunogenic proteins, adhesins or other virulence factors common to capsulated and non-typeable (encapsulated) pneumococcal strains hold promise. All the aforementioned accounts for a need for microbiological monitoring of S. pneumoniae carriage and search for new diagnostic approaches for etiological interpretation of S. pneumoniae-associated diseases.
format	article
author	A. Z. Zaripova Yu. A. Tyurin L. T. Bayazitova O. F. Tyupkina G. Sh. Isaeva
author_facet	A. Z. Zaripova Yu. A. Tyurin L. T. Bayazitova O. F. Tyupkina G. Sh. Isaeva
author_sort	A. Z. Zaripova
title	IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
title_short	IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
title_full	IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
title_fullStr	IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
title_full_unstemmed	IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
title_sort	iga-protease activity coupled to cellular enzymes of different <i>streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/f450bce224fa42b88f2d3e1af92a8662
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IgA-protease activity coupled to cellular enzymes of different <i>Streptococcus pneumonia</i> serotypes isolated in pediatric bacteria carriers

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