Selective microbial genomic DNA isolation using restriction endonucleases.

Selective microbial genomic DNA isolation using restriction endonucleases.

To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI Gm6AT...

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Autores principales: Helen E Barnes, Guohong Liu, Christopher Q Weston, Paula King, Long K Pham, Shannon Waltz, Kimberly T Helzer, Laura Day, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/f4589789518941dbb5e98d04134b80f0
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