Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production

Clostridioides difficile is a major cause of diarrhea in patients with antibiotic administration. Lacticaseibacillus casei T21, isolated from a human gastric biopsy, was tested in a murine C. difficile infection (CDI) model and colonic epithelial cells (Caco-2 and HT-29). Daily administration of L....

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Autores principales:	Wimonrat Panpetch, Pornpimol Phuengmaung, Thanya Cheibchalard, Naraporn Somboonna, Asada Leelahavanichkul, Somying Tumwasorn
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Lacticaseibacillus casei T21 probiotics proinflammatory cytokines Clostridioides difficile gut dysbiosis inflammation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f45e84b7f8604457b05a8c57f1ad23cf
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spelling	oai:doaj.org-article:f45e84b7f8604457b05a8c57f1ad23cf2021-12-02T00:04:32ZLacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production1664-302X10.3389/fmicb.2021.745299https://doaj.org/article/f45e84b7f8604457b05a8c57f1ad23cf2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.745299/fullhttps://doaj.org/toc/1664-302XClostridioides difficile is a major cause of diarrhea in patients with antibiotic administration. Lacticaseibacillus casei T21, isolated from a human gastric biopsy, was tested in a murine C. difficile infection (CDI) model and colonic epithelial cells (Caco-2 and HT-29). Daily administration of L. casei T21 [1 × 108 colony forming units (CFU)/dose] for 4 days starting at 1 day before C. difficile challenge attenuated CDI as demonstrated by a reduction in mortality rate, weight loss, diarrhea, gut leakage, gut dysbiosis, intestinal pathology changes, and levels of pro-inflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor (TNF)-α, macrophage inflammatory protein 2 (MIP-2), and keratinocyte chemoattractant (KC)] in the intestinal tissue and serum. Conditioned media from L. casei T21 exerted biological activities that fight against C. difficile as demonstrated in colonic epithelial cells by the following: (i) suppression of gene expression and production of IL-8, an important chemokine involved in C. difficile pathogenesis, (ii) reduction in the expression of SLC11A1 (solute carrier family 11 member 1) and HuR (human antigen R), important genes for the lethality of C. difficile toxin B, (iii) augmentation of intestinal integrity, and (iv) up-regulation of MUC2, a mucosal protective gene. These results supported the therapeutic potential of L. casei T21 for CDI and the need for further study on the intervention capabilities of CDI.Wimonrat PanpetchPornpimol PhuengmaungThanya CheibchalardNaraporn SomboonnaNaraporn SomboonnaAsada LeelahavanichkulAsada LeelahavanichkulSomying TumwasornSomying TumwasornFrontiers Media S.A.articleLacticaseibacillus casei T21probioticsproinflammatory cytokinesClostridioides difficilegut dysbiosisinflammationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lacticaseibacillus casei T21 probiotics proinflammatory cytokines Clostridioides difficile gut dysbiosis inflammation Microbiology QR1-502
spellingShingle	Lacticaseibacillus casei T21 probiotics proinflammatory cytokines Clostridioides difficile gut dysbiosis inflammation Microbiology QR1-502 Wimonrat Panpetch Pornpimol Phuengmaung Thanya Cheibchalard Naraporn Somboonna Naraporn Somboonna Asada Leelahavanichkul Asada Leelahavanichkul Somying Tumwasorn Somying Tumwasorn Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
description	Clostridioides difficile is a major cause of diarrhea in patients with antibiotic administration. Lacticaseibacillus casei T21, isolated from a human gastric biopsy, was tested in a murine C. difficile infection (CDI) model and colonic epithelial cells (Caco-2 and HT-29). Daily administration of L. casei T21 [1 × 108 colony forming units (CFU)/dose] for 4 days starting at 1 day before C. difficile challenge attenuated CDI as demonstrated by a reduction in mortality rate, weight loss, diarrhea, gut leakage, gut dysbiosis, intestinal pathology changes, and levels of pro-inflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor (TNF)-α, macrophage inflammatory protein 2 (MIP-2), and keratinocyte chemoattractant (KC)] in the intestinal tissue and serum. Conditioned media from L. casei T21 exerted biological activities that fight against C. difficile as demonstrated in colonic epithelial cells by the following: (i) suppression of gene expression and production of IL-8, an important chemokine involved in C. difficile pathogenesis, (ii) reduction in the expression of SLC11A1 (solute carrier family 11 member 1) and HuR (human antigen R), important genes for the lethality of C. difficile toxin B, (iii) augmentation of intestinal integrity, and (iv) up-regulation of MUC2, a mucosal protective gene. These results supported the therapeutic potential of L. casei T21 for CDI and the need for further study on the intervention capabilities of CDI.
format	article
author	Wimonrat Panpetch Pornpimol Phuengmaung Thanya Cheibchalard Naraporn Somboonna Naraporn Somboonna Asada Leelahavanichkul Asada Leelahavanichkul Somying Tumwasorn Somying Tumwasorn
author_facet	Wimonrat Panpetch Pornpimol Phuengmaung Thanya Cheibchalard Naraporn Somboonna Naraporn Somboonna Asada Leelahavanichkul Asada Leelahavanichkul Somying Tumwasorn Somying Tumwasorn
author_sort	Wimonrat Panpetch
title	Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
title_short	Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
title_full	Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
title_fullStr	Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
title_full_unstemmed	Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production
title_sort	lacticaseibacillus casei strain t21 attenuates clostridioides difficile infection in a murine model through reduction of inflammation and gut dysbiosis with decreased toxin lethality and enhanced mucin production
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f45e84b7f8604457b05a8c57f1ad23cf
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Lacticaseibacillus casei Strain T21 Attenuates Clostridioides difficile Infection in a Murine Model Through Reduction of Inflammation and Gut Dysbiosis With Decreased Toxin Lethality and Enhanced Mucin Production

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