Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts

Abstract The effects of spaceflight on human physiology is an increasingly studied field, yet the molecular mechanisms driving physiological changes remain unknown. With that in mind, this study was performed to obtain a deeper understanding of changes to the human proteome during space travel, by q...

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Autores principales:	Irina M. Larina, Andrew J. Percy, Juncong Yang, Christoph H. Borchers, Andrei M. Nosovsky, Anatoli I. Grigoriev, Evgeny N. Nikolaev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f462ad54401c458f8b2253dd84b6c722
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spelling	oai:doaj.org-article:f462ad54401c458f8b2253dd84b6c7222021-12-02T15:06:19ZProtein expression changes caused by spaceflight as measured for 18 Russian cosmonauts10.1038/s41598-017-08432-w2045-2322https://doaj.org/article/f462ad54401c458f8b2253dd84b6c7222017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08432-whttps://doaj.org/toc/2045-2322Abstract The effects of spaceflight on human physiology is an increasingly studied field, yet the molecular mechanisms driving physiological changes remain unknown. With that in mind, this study was performed to obtain a deeper understanding of changes to the human proteome during space travel, by quantitating a panel of 125 proteins in the blood plasma of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station. The panel of labeled prototypic tryptic peptides from these proteins covered a concentration range of more than 5 orders of magnitude in human plasma. Quantitation was achieved by a well-established and highly-regarded targeted mass spectrometry approach involving multiple reaction monitoring in conjunction with stable isotope-labeled standards. Linear discriminant function analysis of the quantitative results revealed three distinct groups of proteins: 1) proteins with post-flight protein concentrations remaining stable, 2) proteins whose concentrations recovered slowly, or 3) proteins whose concentrations recovered rapidly to their pre-flight levels. Using a systems biology approach, nearly all of the reacting proteins could be linked to pathways that regulate the activities of proteases, natural immunity, lipid metabolism, coagulation cascades, or extracellular matrix metabolism.Irina M. LarinaAndrew J. PercyJuncong YangChristoph H. BorchersAndrei M. NosovskyAnatoli I. GrigorievEvgeny N. NikolaevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irina M. Larina Andrew J. Percy Juncong Yang Christoph H. Borchers Andrei M. Nosovsky Anatoli I. Grigoriev Evgeny N. Nikolaev Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
description	Abstract The effects of spaceflight on human physiology is an increasingly studied field, yet the molecular mechanisms driving physiological changes remain unknown. With that in mind, this study was performed to obtain a deeper understanding of changes to the human proteome during space travel, by quantitating a panel of 125 proteins in the blood plasma of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station. The panel of labeled prototypic tryptic peptides from these proteins covered a concentration range of more than 5 orders of magnitude in human plasma. Quantitation was achieved by a well-established and highly-regarded targeted mass spectrometry approach involving multiple reaction monitoring in conjunction with stable isotope-labeled standards. Linear discriminant function analysis of the quantitative results revealed three distinct groups of proteins: 1) proteins with post-flight protein concentrations remaining stable, 2) proteins whose concentrations recovered slowly, or 3) proteins whose concentrations recovered rapidly to their pre-flight levels. Using a systems biology approach, nearly all of the reacting proteins could be linked to pathways that regulate the activities of proteases, natural immunity, lipid metabolism, coagulation cascades, or extracellular matrix metabolism.
format	article
author	Irina M. Larina Andrew J. Percy Juncong Yang Christoph H. Borchers Andrei M. Nosovsky Anatoli I. Grigoriev Evgeny N. Nikolaev
author_facet	Irina M. Larina Andrew J. Percy Juncong Yang Christoph H. Borchers Andrei M. Nosovsky Anatoli I. Grigoriev Evgeny N. Nikolaev
author_sort	Irina M. Larina
title	Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
title_short	Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
title_full	Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
title_fullStr	Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
title_full_unstemmed	Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
title_sort	protein expression changes caused by spaceflight as measured for 18 russian cosmonauts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f462ad54401c458f8b2253dd84b6c722
work_keys_str_mv	AT irinamlarina proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT andrewjpercy proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT juncongyang proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT christophhborchers proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT andreimnosovsky proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT anatoliigrigoriev proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts AT evgenynnikolaev proteinexpressionchangescausedbyspaceflightasmeasuredfor18russiancosmonauts
_version_	1718388554745249792

Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts

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