Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>

Pufferfish is increasingly regarded by many as a delicacy. However, the tetrodotoxin (TTX) that accumulates in its body can be lethal upon consumption by humans. TTX is known to mainly accumulate in pufferfish skin, but the accumulation mechanisms are poorly understood. In this study, we aimed to ex...

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Autores principales:	Huimin Feng, Kun Qiao, Chunchun Wang, Bei Chen, Min Xu, Hua Hao, Zhen Huang, Zhiyu Liu, Qin Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Takifugu</i> <i>flavidus</i> tetrodotoxin transcriptome proteome accumulate skin Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f464cc79ecf542f7a02dbe9a461168e3
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spelling	oai:doaj.org-article:f464cc79ecf542f7a02dbe9a461168e32021-11-25T18:13:00ZMulti-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>10.3390/md191106391660-3397https://doaj.org/article/f464cc79ecf542f7a02dbe9a461168e32021-11-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/639https://doaj.org/toc/1660-3397Pufferfish is increasingly regarded by many as a delicacy. However, the tetrodotoxin (TTX) that accumulates in its body can be lethal upon consumption by humans. TTX is known to mainly accumulate in pufferfish skin, but the accumulation mechanisms are poorly understood. In this study, we aimed to explore the possible mechanism of TTX accumulation in the skin of the pufferfish <i>Takifugu flavidus</i> following treatment with TTX. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we detected 37.3% of toxin accumulated in the skin at the end of the rearing period (168 h). Transcriptome and proteome analyses revealed the mechanism and pathways of TTX accumulation in the skin of <i>T. flavidus</i> in detail. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes analyses strongly suggest that cardiac muscle contraction and adrenergic signaling in cardiomyocyte pathways play an important role in TTX accumulation. Moreover, some upregulated and downregulated genes, which were determined via RNA-Seq, were verified with qPCR analysis. This study is the first to use multi-omics profiling data to identify novel regulatory network mechanisms of TTX accumulation in the skin of pufferfish.Huimin FengKun QiaoChunchun WangBei ChenMin XuHua HaoZhen HuangZhiyu LiuQin WangMDPI AGarticle<i>Takifugu</i> <i>flavidus</i>tetrodotoxintranscriptomeproteomeaccumulateskinBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 639, p 639 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Takifugu</i> <i>flavidus</i> tetrodotoxin transcriptome proteome accumulate skin Biology (General) QH301-705.5
spellingShingle	<i>Takifugu</i> <i>flavidus</i> tetrodotoxin transcriptome proteome accumulate skin Biology (General) QH301-705.5 Huimin Feng Kun Qiao Chunchun Wang Bei Chen Min Xu Hua Hao Zhen Huang Zhiyu Liu Qin Wang Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
description	Pufferfish is increasingly regarded by many as a delicacy. However, the tetrodotoxin (TTX) that accumulates in its body can be lethal upon consumption by humans. TTX is known to mainly accumulate in pufferfish skin, but the accumulation mechanisms are poorly understood. In this study, we aimed to explore the possible mechanism of TTX accumulation in the skin of the pufferfish <i>Takifugu flavidus</i> following treatment with TTX. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we detected 37.3% of toxin accumulated in the skin at the end of the rearing period (168 h). Transcriptome and proteome analyses revealed the mechanism and pathways of TTX accumulation in the skin of <i>T. flavidus</i> in detail. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes analyses strongly suggest that cardiac muscle contraction and adrenergic signaling in cardiomyocyte pathways play an important role in TTX accumulation. Moreover, some upregulated and downregulated genes, which were determined via RNA-Seq, were verified with qPCR analysis. This study is the first to use multi-omics profiling data to identify novel regulatory network mechanisms of TTX accumulation in the skin of pufferfish.
format	article
author	Huimin Feng Kun Qiao Chunchun Wang Bei Chen Min Xu Hua Hao Zhen Huang Zhiyu Liu Qin Wang
author_facet	Huimin Feng Kun Qiao Chunchun Wang Bei Chen Min Xu Hua Hao Zhen Huang Zhiyu Liu Qin Wang
author_sort	Huimin Feng
title	Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
title_short	Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
title_full	Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
title_fullStr	Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
title_full_unstemmed	Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>
title_sort	multi-omics analysis of gene and protein candidates possibly related to tetrodotoxin accumulation in the skin of <i>takifugu flavidus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f464cc79ecf542f7a02dbe9a461168e3
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Multi-Omics Analysis of Gene and Protein Candidates Possibly Related to Tetrodotoxin Accumulation in the Skin of <i>Takifugu flavidus</i>

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