Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.

Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contamin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sujin Baek, Hyun Ho Noh, Chang Jo Kim, Kyungae Son, Hee-Dong Lee, Leesun Kim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f46530802453448999496042f510bf12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f46530802453448999496042f510bf12
record_format	dspace
spelling	oai:doaj.org-article:f46530802453448999496042f510bf122021-12-02T20:16:56ZEasy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.1932-620310.1371/journal.pone.0258266https://doaj.org/article/f46530802453448999496042f510bf122021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258266https://doaj.org/toc/1932-6203Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contaminated with three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the insect. This study was aimed to optimize a simple extraction method combined with liquid chromatography tandem mass spectrometry and apply it to the real samples. After the pulverized samples (5 g) were extracted with acetonitrile (10 mL) and formic acid (100 μL), fat and lipids in the samples were slowly precipitated at -20°C for 24 hours. After eight different clean-up methods were investigated, the mixture of 150 mg MgSO4/25 mg PSA/25 mg C18 was selected due to optimal recovery of the target compounds. Recovery (77.9%‒80.8% for carbendazim, 111.2%‒116.7% for dimethomorph, and 111.9%‒112.5% for fenoxanil) was achieved with reasonable relative standard deviation (<5.5%) The analytical method developed in this study was used to analyze three compounds in the 24 insect samples donated by the insect farm owners but no target compounds were detected. These results can provide important data for establishing the pesticide safety standards for P. brevitarsis before the medical applications.Sujin BaekHyun Ho NohChang Jo KimKyungae SonHee-Dong LeeLeesun KimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258266 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sujin Baek Hyun Ho Noh Chang Jo Kim Kyungae Son Hee-Dong Lee Leesun Kim Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
description	Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contaminated with three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the insect. This study was aimed to optimize a simple extraction method combined with liquid chromatography tandem mass spectrometry and apply it to the real samples. After the pulverized samples (5 g) were extracted with acetonitrile (10 mL) and formic acid (100 μL), fat and lipids in the samples were slowly precipitated at -20°C for 24 hours. After eight different clean-up methods were investigated, the mixture of 150 mg MgSO4/25 mg PSA/25 mg C18 was selected due to optimal recovery of the target compounds. Recovery (77.9%‒80.8% for carbendazim, 111.2%‒116.7% for dimethomorph, and 111.9%‒112.5% for fenoxanil) was achieved with reasonable relative standard deviation (<5.5%) The analytical method developed in this study was used to analyze three compounds in the 24 insect samples donated by the insect farm owners but no target compounds were detected. These results can provide important data for establishing the pesticide safety standards for P. brevitarsis before the medical applications.
format	article
author	Sujin Baek Hyun Ho Noh Chang Jo Kim Kyungae Son Hee-Dong Lee Leesun Kim
author_facet	Sujin Baek Hyun Ho Noh Chang Jo Kim Kyungae Son Hee-Dong Lee Leesun Kim
author_sort	Sujin Baek
title	Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
title_short	Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
title_full	Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
title_fullStr	Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
title_full_unstemmed	Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.
title_sort	easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from protaetia brevitarsis seulensis using lc-ms/ms.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f46530802453448999496042f510bf12
work_keys_str_mv	AT sujinbaek easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms AT hyunhonoh easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms AT changjokim easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms AT kyungaeson easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms AT heedonglee easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms AT leesunkim easyandeffectiveanalyticalmethodofcarbendazimdimethomorphandfenoxanilfromprotaetiabrevitarsisseulensisusinglcmsms
_version_	1718374438791020544

Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS.

Ejemplares similares