Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Abstract In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to z...

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Autores principales:	Silvia Falcinelli, Ana Rodiles, Azadeh Hatef, Simona Picchietti, Lina Cossignani, Daniel L. Merrifield, Suraj Unniappan, Oliana Carnevali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f46c6fb0d7dd48229cd240946aa41b02
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spelling	oai:doaj.org-article:f46c6fb0d7dd48229cd240946aa41b022021-12-02T12:32:14ZDietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish10.1038/s41598-017-05147-w2045-2322https://doaj.org/article/f46c6fb0d7dd48229cd240946aa41b022017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05147-whttps://doaj.org/toc/2045-2322Abstract In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG. Probiotic feeding also decreased nesfatin-1 peptide in HFD-P and attenuated weight gain in HFD-P and MFD-P fed zebrafish, but not in LFD-P group. Intestinal ultrastructure was not affected by dietary fat level or probiotic inclusion. In conclusion, these findings underline the role of fat content in the diet in altering gut microbiota community by shifting phylotype composition and highlight the potential of probiotics to attenuate high-fat diet-related metabolic disorder.Silvia FalcinelliAna RodilesAzadeh HatefSimona PicchiettiLina CossignaniDaniel L. MerrifieldSuraj UnniappanOliana CarnevaliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Falcinelli Ana Rodiles Azadeh Hatef Simona Picchietti Lina Cossignani Daniel L. Merrifield Suraj Unniappan Oliana Carnevali Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
description	Abstract In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG. Probiotic feeding also decreased nesfatin-1 peptide in HFD-P and attenuated weight gain in HFD-P and MFD-P fed zebrafish, but not in LFD-P group. Intestinal ultrastructure was not affected by dietary fat level or probiotic inclusion. In conclusion, these findings underline the role of fat content in the diet in altering gut microbiota community by shifting phylotype composition and highlight the potential of probiotics to attenuate high-fat diet-related metabolic disorder.
format	article
author	Silvia Falcinelli Ana Rodiles Azadeh Hatef Simona Picchietti Lina Cossignani Daniel L. Merrifield Suraj Unniappan Oliana Carnevali
author_facet	Silvia Falcinelli Ana Rodiles Azadeh Hatef Simona Picchietti Lina Cossignani Daniel L. Merrifield Suraj Unniappan Oliana Carnevali
author_sort	Silvia Falcinelli
title	Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
title_short	Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
title_full	Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
title_fullStr	Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
title_full_unstemmed	Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
title_sort	dietary lipid content reorganizes gut microbiota and probiotic l. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f46c6fb0d7dd48229cd240946aa41b02
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Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

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