Aggregatibacter actinomycetemcomitans and Aggregatibacter aphrophilus in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures

Background: A virulent genotype (JP2) of the periodonto-pathogen, Aggregatibacter actinomycetemcomitans (Aa), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with per...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mark Lindholm, Rolf Claesson, Arthur Kemoli, Tonnie Mulli, Jan Oscarsson, Dorte Haubek, Anders Johansson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aggregatibacter actinomycetemcomitans</i> <i>Aggregatibacter aphrophilus</i> leukotoxicity herbal plants Maasai adolescents Medicine R
Acceso en línea:	https://doaj.org/article/f46e0d7b086c432bb04e73d48018880b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f46e0d7b086c432bb04e73d48018880b
record_format	dspace
spelling	oai:doaj.org-article:f46e0d7b086c432bb04e73d48018880b2021-11-25T18:02:27Z<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures10.3390/jcm102254022077-0383https://doaj.org/article/f46e0d7b086c432bb04e73d48018880b2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/22/5402https://doaj.org/toc/2077-0383Background: A virulent genotype (JP2) of the periodonto-pathogen, <i>Aggregatibacter actinomycetemcomitans</i> (<i>Aa</i>), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a high leukotoxicity. The aim of the study was to examine the prevalence of <i>Aa</i> and its JP2 genotype, the prevalence of the oral, commensal <i>Aggregatibacter aphrophilus</i> in a Maasai adolescent population, and the effect of herbal plants for inhibition of leukotoxicity. Methods: A total of 284 adolescents from Maasai Mara, Kenya, underwent an oral examination and microbial sampling. The presence of <i>Aa</i> and <i>A. aphrophilus</i> was analyzed by quantitative PCR and cultivation (the 58 samples collected at the last day of field study). The collected <i>Aa</i> strains were characterized and leukotoxin promoter typed. Additionally, herbal plants commonly used for oral hygiene were assessed for the inhibition of leukotoxicity. Results and Conclusions: The prevalence of <i>Aa</i> in stimulated whole saliva was high (71.8%), with the JP2 genotype detected in one individual, and <i>A. aphrophilus</i> in 99% of the sampled individuals. The commonly used herbal plant, <i>Warburgia ugandensis</i>, inactivated <i>Aa</i> leukotoxicity. The <i>Aa</i> virulence might be reduced through use of <i>W. ugandensis</i> and the high levels of <i>A. aphrophilus</i>.Mark LindholmRolf ClaessonArthur KemoliTonnie MulliJan OscarssonDorte HaubekAnders JohanssonMDPI AGarticle<i>Aggregatibacter actinomycetemcomitans</i><i>Aggregatibacter aphrophilus</i>leukotoxicityherbal plantsMaasai adolescentsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5402, p 5402 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aggregatibacter actinomycetemcomitans</i> <i>Aggregatibacter aphrophilus</i> leukotoxicity herbal plants Maasai adolescents Medicine R
spellingShingle	<i>Aggregatibacter actinomycetemcomitans</i> <i>Aggregatibacter aphrophilus</i> leukotoxicity herbal plants Maasai adolescents Medicine R Mark Lindholm Rolf Claesson Arthur Kemoli Tonnie Mulli Jan Oscarsson Dorte Haubek Anders Johansson <i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
description	Background: A virulent genotype (JP2) of the periodonto-pathogen, <i>Aggregatibacter actinomycetemcomitans</i> (<i>Aa</i>), is widespread in North and West Africa, while its presence in East Africa has not been thoroughly investigated. This JP2 genotype is associated with periodontitis in adolescents and has a high leukotoxicity. The aim of the study was to examine the prevalence of <i>Aa</i> and its JP2 genotype, the prevalence of the oral, commensal <i>Aggregatibacter aphrophilus</i> in a Maasai adolescent population, and the effect of herbal plants for inhibition of leukotoxicity. Methods: A total of 284 adolescents from Maasai Mara, Kenya, underwent an oral examination and microbial sampling. The presence of <i>Aa</i> and <i>A. aphrophilus</i> was analyzed by quantitative PCR and cultivation (the 58 samples collected at the last day of field study). The collected <i>Aa</i> strains were characterized and leukotoxin promoter typed. Additionally, herbal plants commonly used for oral hygiene were assessed for the inhibition of leukotoxicity. Results and Conclusions: The prevalence of <i>Aa</i> in stimulated whole saliva was high (71.8%), with the JP2 genotype detected in one individual, and <i>A. aphrophilus</i> in 99% of the sampled individuals. The commonly used herbal plant, <i>Warburgia ugandensis</i>, inactivated <i>Aa</i> leukotoxicity. The <i>Aa</i> virulence might be reduced through use of <i>W. ugandensis</i> and the high levels of <i>A. aphrophilus</i>.
format	article
author	Mark Lindholm Rolf Claesson Arthur Kemoli Tonnie Mulli Jan Oscarsson Dorte Haubek Anders Johansson
author_facet	Mark Lindholm Rolf Claesson Arthur Kemoli Tonnie Mulli Jan Oscarsson Dorte Haubek Anders Johansson
author_sort	Mark Lindholm
title	<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_short	<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_full	<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_fullStr	<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_full_unstemmed	<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures
title_sort	<i>aggregatibacter actinomycetemcomitans</i> and <i>aggregatibacter aphrophilus</i> in a kenyan maasai adolescent population and inhibition of leukotoxic activity by herbal plants used as part of oral hygiene procedures
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f46e0d7b086c432bb04e73d48018880b
work_keys_str_mv	AT marklindholm iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT rolfclaesson iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT arthurkemoli iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT tonniemulli iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT janoscarsson iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT dortehaubek iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures AT andersjohansson iaggregatibacteractinomycetemcomitansiandiaggregatibacteraphrophilusiinakenyanmaasaiadolescentpopulationandinhibitionofleukotoxicactivitybyherbalplantsusedaspartoforalhygieneprocedures
_version_	1718411718056476672

<i>Aggregatibacter actinomycetemcomitans</i> and <i>Aggregatibacter aphrophilus</i> in a Kenyan Maasai Adolescent Population and Inhibition of Leukotoxic Activity by Herbal Plants Used as Part of Oral Hygiene Procedures

Ejemplares similares