Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.

<h4>Introduction</h4>Yes-associated protein (YAP) is considered an oncogene found amplified in multiple tumors, including head and neck squamous cell carcinoma (HNSCC). However, the role for YAP expression in HNSCC is not understood. Based on the central role of YAP in the hippo pathway,...

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Autores principales:	Lin Ge, Matthew Smail, Wenxia Meng, Yu Shyr, Fei Ye, Kang-Hsien Fan, Xiaohong Li, Hong-Mei Zhou, Neil A Bhowmick
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f47a2f0730d24dd89db9cb03ae33abaa
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spelling	oai:doaj.org-article:f47a2f0730d24dd89db9cb03ae33abaa2021-11-18T07:34:37ZYes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.1932-620310.1371/journal.pone.0027529https://doaj.org/article/f47a2f0730d24dd89db9cb03ae33abaa2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22096589/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Yes-associated protein (YAP) is considered an oncogene found amplified in multiple tumors, including head and neck squamous cell carcinoma (HNSCC). However, the role for YAP expression in HNSCC is not understood. Based on the central role of YAP in the hippo pathway, we tested if YAP was associated with the stage of HNSCC progression and metastatic potential.<h4>Methods</h4>To determine the expression of YAP in human benign and HNSCC tissue specimens, immunohistochemical analyses were performed in whole tissue samples and tissue microarrays. The expression of YAP in tissues of microarray was first associated with clinic-pathologic factors and results verified in samples from whole tissue sections. To investigate the role of YAP and p63 in regulating HNSCC epithelial to mesenchymal transition, epithelial and mesenchymal markers were assayed in Fadu and SCC-25 cells, HNSCC cells with endogenously elevated YAP expression and siRNA-mediated expression knockdown.<h4>Results</h4>Analysis of human HNSCC tissues suggested YAP expression was elevated in tumors compared to benign tissues and specifically localized at the tumor invasive front (p value < 0.05). But, indexed YAP expression was lower with greater tumor grade (p value = 0.02). In contrast, p63 expression was primarily elevated in high-grade tumors. Interestingly, both YAP and p63 was strongly expressed at the tumor invasive front and in metastatic HNSCC. Strikingly, we demonstrated YAP expression in the primary HNSCC tumor was associated with nodal metastasis in univariate analysis (p value = 0.02). However, the knockdown of YAP in Fadu and SCC-25 cell lines was not associated with changes in epithelial to mesenchymal transdifferentiation or p63 expression.<h4>Conclusion</h4>Together, YAP expression, in combination with p63 can facilitate identification of HNSCC tumors from hyperplastic and benign tissues and the metastatic function of YAP in HNSCC may not be a result of epithelia to mesenchymal transdifferentiation.Lin GeMatthew SmailWenxia MengYu ShyrFei YeKang-Hsien FanXiaohong LiHong-Mei ZhouNeil A BhowmickPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27529 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Ge Matthew Smail Wenxia Meng Yu Shyr Fei Ye Kang-Hsien Fan Xiaohong Li Hong-Mei Zhou Neil A Bhowmick Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
description	<h4>Introduction</h4>Yes-associated protein (YAP) is considered an oncogene found amplified in multiple tumors, including head and neck squamous cell carcinoma (HNSCC). However, the role for YAP expression in HNSCC is not understood. Based on the central role of YAP in the hippo pathway, we tested if YAP was associated with the stage of HNSCC progression and metastatic potential.<h4>Methods</h4>To determine the expression of YAP in human benign and HNSCC tissue specimens, immunohistochemical analyses were performed in whole tissue samples and tissue microarrays. The expression of YAP in tissues of microarray was first associated with clinic-pathologic factors and results verified in samples from whole tissue sections. To investigate the role of YAP and p63 in regulating HNSCC epithelial to mesenchymal transition, epithelial and mesenchymal markers were assayed in Fadu and SCC-25 cells, HNSCC cells with endogenously elevated YAP expression and siRNA-mediated expression knockdown.<h4>Results</h4>Analysis of human HNSCC tissues suggested YAP expression was elevated in tumors compared to benign tissues and specifically localized at the tumor invasive front (p value < 0.05). But, indexed YAP expression was lower with greater tumor grade (p value = 0.02). In contrast, p63 expression was primarily elevated in high-grade tumors. Interestingly, both YAP and p63 was strongly expressed at the tumor invasive front and in metastatic HNSCC. Strikingly, we demonstrated YAP expression in the primary HNSCC tumor was associated with nodal metastasis in univariate analysis (p value = 0.02). However, the knockdown of YAP in Fadu and SCC-25 cell lines was not associated with changes in epithelial to mesenchymal transdifferentiation or p63 expression.<h4>Conclusion</h4>Together, YAP expression, in combination with p63 can facilitate identification of HNSCC tumors from hyperplastic and benign tissues and the metastatic function of YAP in HNSCC may not be a result of epithelia to mesenchymal transdifferentiation.
format	article
author	Lin Ge Matthew Smail Wenxia Meng Yu Shyr Fei Ye Kang-Hsien Fan Xiaohong Li Hong-Mei Zhou Neil A Bhowmick
author_facet	Lin Ge Matthew Smail Wenxia Meng Yu Shyr Fei Ye Kang-Hsien Fan Xiaohong Li Hong-Mei Zhou Neil A Bhowmick
author_sort	Lin Ge
title	Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
title_short	Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
title_full	Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
title_fullStr	Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
title_full_unstemmed	Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
title_sort	yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f47a2f0730d24dd89db9cb03ae33abaa
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Yes-associated protein expression in head and neck squamous cell carcinoma nodal metastasis.

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