Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

Abstract The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence...

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Autores principales:	Tania García-Martínez, Teresa Mogas, Steven F. Mullen, Iris Martínez-Rodero, Ramila E. Gulieva, Adam Z. Higgins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f47b10002b8a48829e4f8226f2522032
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spelling	oai:doaj.org-article:f47b10002b8a48829e4f8226f25220322021-12-02T16:06:44ZEffect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches10.1038/s41598-021-94884-02045-2322https://doaj.org/article/f47b10002b8a48829e4f8226f25220322021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94884-0https://doaj.org/toc/2045-2322Abstract The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence of ethylene glycol (EG) and dimethyl sulphoxide (Me2SO), and to compare two different modeling approaches: the two parameter (2P) model and a nondilute transport model. Membrane permeability parameters were determined by consecutively exposing oocytes to increasing concentrations of Me2SO or EG. Higher water permeability was observed for MII oocytes than GV oocytes in the presence of both Me2SO and EG, and in all cases the water permeability was observed to decrease as CPA concentration increased. At high CPA concentrations, the CPA permeability was similar for Me2SO and EG, for both MII and GV oocytes, but at low concentrations the EG permeability of GV oocytes was substantially higher. Predictions of cell volume changes during CPA addition and removal indicate that accounting for the concentration dependence of permeability only has a modest effect, but there were substantial differences between the 2P model and the nondilute model during CPA removal, which may have implications for design of improved methods for bovine oocyte vitrification.Tania García-MartínezTeresa MogasSteven F. MullenIris Martínez-RoderoRamila E. GulievaAdam Z. HigginsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tania García-Martínez Teresa Mogas Steven F. Mullen Iris Martínez-Rodero Ramila E. Gulieva Adam Z. Higgins Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
description	Abstract The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence of ethylene glycol (EG) and dimethyl sulphoxide (Me2SO), and to compare two different modeling approaches: the two parameter (2P) model and a nondilute transport model. Membrane permeability parameters were determined by consecutively exposing oocytes to increasing concentrations of Me2SO or EG. Higher water permeability was observed for MII oocytes than GV oocytes in the presence of both Me2SO and EG, and in all cases the water permeability was observed to decrease as CPA concentration increased. At high CPA concentrations, the CPA permeability was similar for Me2SO and EG, for both MII and GV oocytes, but at low concentrations the EG permeability of GV oocytes was substantially higher. Predictions of cell volume changes during CPA addition and removal indicate that accounting for the concentration dependence of permeability only has a modest effect, but there were substantial differences between the 2P model and the nondilute model during CPA removal, which may have implications for design of improved methods for bovine oocyte vitrification.
format	article
author	Tania García-Martínez Teresa Mogas Steven F. Mullen Iris Martínez-Rodero Ramila E. Gulieva Adam Z. Higgins
author_facet	Tania García-Martínez Teresa Mogas Steven F. Mullen Iris Martínez-Rodero Ramila E. Gulieva Adam Z. Higgins
author_sort	Tania García-Martínez
title	Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
title_short	Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
title_full	Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
title_fullStr	Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
title_full_unstemmed	Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
title_sort	effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f47b10002b8a48829e4f8226f2522032
work_keys_str_mv	AT taniagarciamartinez effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches AT teresamogas effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches AT stevenfmullen effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches AT irismartinezrodero effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches AT ramilaegulieva effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches AT adamzhiggins effectofcryoprotectantconcentrationonbovineoocytepermeabilityandcomparisonoftwomembranepermeabilitymodellingapproaches
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Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

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