FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER

Colorectal cancer is one of the most common malignant diseases in Russia worldwide making up 5-6% of all human malignant tumors. Neutrophilic granulocytes are actively involved in development of antitumor response. A key role in tumor regression is assigned to active forms of oxygen produced by neut...

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Autores principales:	O. V. Smirnova, E. V. Kasparov, Ya. I. Perepechay, A. A. Nesytykh, V. S. Belyaev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	spontaneous chemiluminescent activity induced chemiluminescent activity neutrophil granulocytes rectal cancer Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f48431f86f004ce59d3a27003558c57b
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spelling	oai:doaj.org-article:f48431f86f004ce59d3a27003558c57b2021-11-18T08:03:48ZFEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER1563-06252313-741X10.15789/1563-0625-2019-1-157-164https://doaj.org/article/f48431f86f004ce59d3a27003558c57b2019-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1710https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XColorectal cancer is one of the most common malignant diseases in Russia worldwide making up 5-6% of all human malignant tumors. Neutrophilic granulocytes are actively involved in development of antitumor response. A key role in tumor regression is assigned to active forms of oxygen produced by neutrophils. In connection with these pre-requisites, our goal was to study functional characteristics of spontaneous and induced chemiluminescent activity of neutrophil granulocytes in patients with rectal cancer before starting pathogenetic therapy and in subsequent dynamics. The paper presents some laboratory results, i.e., functional indices of neutrophilic granulocytes’ activity in 36 patients with rectal cancer being at different stages of oncological process. The control group consisted of 112 practically healthy volunteers, comparable in sex and age to the group of patients under study. To perform the study venous blood was taken from patients to vacuum test tubes with lithium heparin in the morning time before surgical treatment, and on day 7 after the surgical intervention. Evaluation of spontaneous and induced chemiluminescence was performed for 90 minutes in a 36-channel “CL 3606” chemiluminescence analyzer (Russia). The following characteristics were determined: time of the curve transition to maximal chemiluminescence intensity (Tmax), maximal value of chemiluminescence intensity (Imax), integral area under the chemiluminescence curve (S). Luminol was used as the chemiluminescence enhancer. Opsonized zymosan was used to induce the respiratory explosion. Chemiluminescence amplification induced by opsonized zymosan was evaluated by the ratio of induced-tospontaneous chemiluminescence (Sind/spont) designated as an activation index.Analysis of chemiluminescence activity in neutrophilic granulocytes showed a significant increase in spontaneous chemiluminescence activity at the stages III and IV of the disease. The production of active oxygen forms induced in neutrophilic granulocytes by opsonized zymosan increased in all the study groups, relative to control parameters. The area under the curves of spontaneous and induced chemiluminescence in patients with colorectal cancer at all stages of the oncological process is less, as compared to the control group, which, despite high indices of maximal chemiluminescence activity, may indicate insufficient total production of reactive oxygen species. The time-to-peak values of the chemiluminescence curves in patients with rectal cancer at all stages of the disease did not show statistically significant differences from the control group.O. V. SmirnovaE. V. KasparovYa. I. PerepechayA. A. NesytykhV. S. BelyaevSPb RAACIarticlespontaneous chemiluminescent activityinduced chemiluminescent activityneutrophil granulocytesrectal cancerImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 1, Pp 157-164 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	spontaneous chemiluminescent activity induced chemiluminescent activity neutrophil granulocytes rectal cancer Immunologic diseases. Allergy RC581-607
spellingShingle	spontaneous chemiluminescent activity induced chemiluminescent activity neutrophil granulocytes rectal cancer Immunologic diseases. Allergy RC581-607 O. V. Smirnova E. V. Kasparov Ya. I. Perepechay A. A. Nesytykh V. S. Belyaev FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
description	Colorectal cancer is one of the most common malignant diseases in Russia worldwide making up 5-6% of all human malignant tumors. Neutrophilic granulocytes are actively involved in development of antitumor response. A key role in tumor regression is assigned to active forms of oxygen produced by neutrophils. In connection with these pre-requisites, our goal was to study functional characteristics of spontaneous and induced chemiluminescent activity of neutrophil granulocytes in patients with rectal cancer before starting pathogenetic therapy and in subsequent dynamics. The paper presents some laboratory results, i.e., functional indices of neutrophilic granulocytes’ activity in 36 patients with rectal cancer being at different stages of oncological process. The control group consisted of 112 practically healthy volunteers, comparable in sex and age to the group of patients under study. To perform the study venous blood was taken from patients to vacuum test tubes with lithium heparin in the morning time before surgical treatment, and on day 7 after the surgical intervention. Evaluation of spontaneous and induced chemiluminescence was performed for 90 minutes in a 36-channel “CL 3606” chemiluminescence analyzer (Russia). The following characteristics were determined: time of the curve transition to maximal chemiluminescence intensity (Tmax), maximal value of chemiluminescence intensity (Imax), integral area under the chemiluminescence curve (S). Luminol was used as the chemiluminescence enhancer. Opsonized zymosan was used to induce the respiratory explosion. Chemiluminescence amplification induced by opsonized zymosan was evaluated by the ratio of induced-tospontaneous chemiluminescence (Sind/spont) designated as an activation index.Analysis of chemiluminescence activity in neutrophilic granulocytes showed a significant increase in spontaneous chemiluminescence activity at the stages III and IV of the disease. The production of active oxygen forms induced in neutrophilic granulocytes by opsonized zymosan increased in all the study groups, relative to control parameters. The area under the curves of spontaneous and induced chemiluminescence in patients with colorectal cancer at all stages of the oncological process is less, as compared to the control group, which, despite high indices of maximal chemiluminescence activity, may indicate insufficient total production of reactive oxygen species. The time-to-peak values of the chemiluminescence curves in patients with rectal cancer at all stages of the disease did not show statistically significant differences from the control group.
format	article
author	O. V. Smirnova E. V. Kasparov Ya. I. Perepechay A. A. Nesytykh V. S. Belyaev
author_facet	O. V. Smirnova E. V. Kasparov Ya. I. Perepechay A. A. Nesytykh V. S. Belyaev
author_sort	O. V. Smirnova
title	FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
title_short	FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
title_full	FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
title_fullStr	FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
title_full_unstemmed	FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER
title_sort	features of neutrophil chemiluminescence in the patients with advanced rectal cancer
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/f48431f86f004ce59d3a27003558c57b
work_keys_str_mv	AT ovsmirnova featuresofneutrophilchemiluminescenceinthepatientswithadvancedrectalcancer AT evkasparov featuresofneutrophilchemiluminescenceinthepatientswithadvancedrectalcancer AT yaiperepechay featuresofneutrophilchemiluminescenceinthepatientswithadvancedrectalcancer AT aanesytykh featuresofneutrophilchemiluminescenceinthepatientswithadvancedrectalcancer AT vsbelyaev featuresofneutrophilchemiluminescenceinthepatientswithadvancedrectalcancer
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FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER

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