Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)

Six rhizobia-like-bacterial strains in total, secluded from the root and stem nodules of various leguminous plants were characterized for growth promoting ability on ICCV 2 variety of chickpea. Bacterial strains showed production of IAA, NH3, siderophore, HCN, ACC deaminase, hydrolytic enzyme produc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohammad Imran Mir, B. Kiran Kumar, Subramaniam Gopalakrishnan, Srinivas Vadlamudi, Bee Hameeda
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Root-stem nodules Rhizobium spp. PGP traits 16S rRNA Chickpea SEM analysis Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/f487ad38f70944ac83bb36aca7548c20
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f487ad38f70944ac83bb36aca7548c20
record_format	dspace
spelling	oai:doaj.org-article:f487ad38f70944ac83bb36aca7548c202021-12-02T05:02:37ZCharacterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)2405-844010.1016/j.heliyon.2021.e08321https://doaj.org/article/f487ad38f70944ac83bb36aca7548c202021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024245https://doaj.org/toc/2405-8440Six rhizobia-like-bacterial strains in total, secluded from the root and stem nodules of various leguminous plants were characterized for growth promoting ability on ICCV 2 variety of chickpea. Bacterial strains showed production of IAA, NH3, siderophore, HCN, ACC deaminase, hydrolytic enzyme production such as chitinase, amylase, protease, lipase, β-1, 3-glucanase and solubilization of nutrients such as phosphate, zinc and potassium. However the performance of PGP traits characterized in-vitro varied among the six bacterial strains. The sequences of 16S rRNA gene of bacterial strains IHSR, IHRG, IHAA, IHGN-3, IHCP-1 and IHCP-2 showed maximum identity with Rhizobium sp., Rhizobium tropici, Rhizobium multihospitium, Mesorhizobium sp., Burkholderia cepacia and Rhizobium pusense. In plate culture conditions the bacterial strains changed the colour of media (NFB) from green to blue and showed amplification of nifH gene by PCR, and also enhanced nodule formation in chickpea under greenhouse conditions, which explains their nitrogen fixing ability. Scanning electron microscopy studies of chickpea roots showed colonization by all the six bacterial strains in solo and by consortium (IHRG + IHGN-3). Under greenhouse conditions, chickpea plants inoculated with different strains showed improvement in plant height, number of branches, total chlorophyll, nodule number, nodule weight, shoot weight, root weight, root volume and root surface area at 30 and 45 days after sowing (DAS) over the uninoculated control plants. It was also observed at the crop maturity stage all the bacterial strains inoculated separately enhanced pod number, seed number and total NPK compared to uninoculated control plants. This study suggests that bacteria associated with root and stem nodules can be a promising resource to enhance nodulation, PGP and crop yields in chickpea.Mohammad Imran MirB. Kiran KumarSubramaniam GopalakrishnanSrinivas VadlamudiBee HameedaElsevierarticleRoot-stem nodulesRhizobium spp.PGP traits16S rRNAChickpeaSEM analysisScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08321- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Root-stem nodules Rhizobium spp. PGP traits 16S rRNA Chickpea SEM analysis Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Root-stem nodules Rhizobium spp. PGP traits 16S rRNA Chickpea SEM analysis Science (General) Q1-390 Social sciences (General) H1-99 Mohammad Imran Mir B. Kiran Kumar Subramaniam Gopalakrishnan Srinivas Vadlamudi Bee Hameeda Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
description	Six rhizobia-like-bacterial strains in total, secluded from the root and stem nodules of various leguminous plants were characterized for growth promoting ability on ICCV 2 variety of chickpea. Bacterial strains showed production of IAA, NH3, siderophore, HCN, ACC deaminase, hydrolytic enzyme production such as chitinase, amylase, protease, lipase, β-1, 3-glucanase and solubilization of nutrients such as phosphate, zinc and potassium. However the performance of PGP traits characterized in-vitro varied among the six bacterial strains. The sequences of 16S rRNA gene of bacterial strains IHSR, IHRG, IHAA, IHGN-3, IHCP-1 and IHCP-2 showed maximum identity with Rhizobium sp., Rhizobium tropici, Rhizobium multihospitium, Mesorhizobium sp., Burkholderia cepacia and Rhizobium pusense. In plate culture conditions the bacterial strains changed the colour of media (NFB) from green to blue and showed amplification of nifH gene by PCR, and also enhanced nodule formation in chickpea under greenhouse conditions, which explains their nitrogen fixing ability. Scanning electron microscopy studies of chickpea roots showed colonization by all the six bacterial strains in solo and by consortium (IHRG + IHGN-3). Under greenhouse conditions, chickpea plants inoculated with different strains showed improvement in plant height, number of branches, total chlorophyll, nodule number, nodule weight, shoot weight, root weight, root volume and root surface area at 30 and 45 days after sowing (DAS) over the uninoculated control plants. It was also observed at the crop maturity stage all the bacterial strains inoculated separately enhanced pod number, seed number and total NPK compared to uninoculated control plants. This study suggests that bacteria associated with root and stem nodules can be a promising resource to enhance nodulation, PGP and crop yields in chickpea.
format	article
author	Mohammad Imran Mir B. Kiran Kumar Subramaniam Gopalakrishnan Srinivas Vadlamudi Bee Hameeda
author_facet	Mohammad Imran Mir B. Kiran Kumar Subramaniam Gopalakrishnan Srinivas Vadlamudi Bee Hameeda
author_sort	Mohammad Imran Mir
title	Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
title_short	Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
title_full	Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
title_fullStr	Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
title_full_unstemmed	Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)
title_sort	characterization of rhizobia isolated from leguminous plants and their impact on the growth of iccv 2 variety of chickpea (cicer arietinum l.)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f487ad38f70944ac83bb36aca7548c20
work_keys_str_mv	AT mohammadimranmir characterizationofrhizobiaisolatedfromleguminousplantsandtheirimpactonthegrowthoficcv2varietyofchickpeacicerarietinuml AT bkirankumar characterizationofrhizobiaisolatedfromleguminousplantsandtheirimpactonthegrowthoficcv2varietyofchickpeacicerarietinuml AT subramaniamgopalakrishnan characterizationofrhizobiaisolatedfromleguminousplantsandtheirimpactonthegrowthoficcv2varietyofchickpeacicerarietinuml AT srinivasvadlamudi characterizationofrhizobiaisolatedfromleguminousplantsandtheirimpactonthegrowthoficcv2varietyofchickpeacicerarietinuml AT beehameeda characterizationofrhizobiaisolatedfromleguminousplantsandtheirimpactonthegrowthoficcv2varietyofchickpeacicerarietinuml
_version_	1718400748041011200

Characterization of rhizobia isolated from leguminous plants and their impact on the growth of ICCV 2 variety of chickpea (Cicer arietinum L.)

Ejemplares similares