Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.

<h4>Background</h4>Prostate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected ca...

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Autores principales:	Xiaolong Qian, Changling Li, Bo Pang, Meng Xue, Jian Wang, Jianguang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f48d5cbebbf94576bb2a71d921f27ca4
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spelling	oai:doaj.org-article:f48d5cbebbf94576bb2a71d921f27ca42021-11-18T07:18:37ZSpondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.1932-620310.1371/journal.pone.0037225https://doaj.org/article/f48d5cbebbf94576bb2a71d921f27ca42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22615945/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Prostate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected candidate molecules, and then confirmed their clinical value.<h4>Methodology/principal findings</h4>We first identified extracellular proteins by two-dimensional gel electrophoresis (2-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. We then validated the secreted proteins on a cellular level, and finally determined whether they could be used as PCa diagnostic biomarkers using prostate tissue and serum specimens of Chinese volunteers by immunohistostaining and sandwich ELISA. We obtained credible extracellular protein 2-DE graphs of prostate cell lines. The 5 spots that showed superior repeatability were selected for LC-MS/MS analysis, which identified seven candidate molecules. One of the candidate molecules, spondin-2 (SPON2), was only expressed in the conditioned media (CM) of androgen receptor (AR) positive PCa cell lines. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in PCa. SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. By receiver operator characteristic (ROC) curve analysis, we found that the serum SPON2 level was elevated in PCa patients, showing sensitivity and specificity suitable for diagnostic use. We also found that SPON2 could be used to identify PCa patients with serum PSA levels no higher than 10 ng/ml from healthy elderly men.<h4>Conclusion/significance</h4>SPON2 is a new serum and histological diagnostic biomarker for PCa. It can avoid some of the problems of PSA testing and was here found to offer relatively high sensitivity and specificity relative to PSA.Xiaolong QianChangling LiBo PangMeng XueJian WangJianguang ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37225 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaolong Qian Changling Li Bo Pang Meng Xue Jian Wang Jianguang Zhou Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
description	<h4>Background</h4>Prostate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected candidate molecules, and then confirmed their clinical value.<h4>Methodology/principal findings</h4>We first identified extracellular proteins by two-dimensional gel electrophoresis (2-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. We then validated the secreted proteins on a cellular level, and finally determined whether they could be used as PCa diagnostic biomarkers using prostate tissue and serum specimens of Chinese volunteers by immunohistostaining and sandwich ELISA. We obtained credible extracellular protein 2-DE graphs of prostate cell lines. The 5 spots that showed superior repeatability were selected for LC-MS/MS analysis, which identified seven candidate molecules. One of the candidate molecules, spondin-2 (SPON2), was only expressed in the conditioned media (CM) of androgen receptor (AR) positive PCa cell lines. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in PCa. SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. By receiver operator characteristic (ROC) curve analysis, we found that the serum SPON2 level was elevated in PCa patients, showing sensitivity and specificity suitable for diagnostic use. We also found that SPON2 could be used to identify PCa patients with serum PSA levels no higher than 10 ng/ml from healthy elderly men.<h4>Conclusion/significance</h4>SPON2 is a new serum and histological diagnostic biomarker for PCa. It can avoid some of the problems of PSA testing and was here found to offer relatively high sensitivity and specificity relative to PSA.
format	article
author	Xiaolong Qian Changling Li Bo Pang Meng Xue Jian Wang Jianguang Zhou
author_facet	Xiaolong Qian Changling Li Bo Pang Meng Xue Jian Wang Jianguang Zhou
author_sort	Xiaolong Qian
title	Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
title_short	Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
title_full	Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
title_fullStr	Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
title_full_unstemmed	Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.
title_sort	spondin-2 (spon2), a more prostate-cancer-specific diagnostic biomarker.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f48d5cbebbf94576bb2a71d921f27ca4
work_keys_str_mv	AT xiaolongqian spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker AT changlingli spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker AT bopang spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker AT mengxue spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker AT jianwang spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker AT jianguangzhou spondin2spon2amoreprostatecancerspecificdiagnosticbiomarker
_version_	1718423701335048192

Spondin-2 (SPON2), a more prostate-cancer-specific diagnostic biomarker.

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