Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested inves...

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Autores principales:	Faten Ghodhbane-Gtari, Timothy D’Angelo, Abdellatif Gueddou, Sabrine Ghazouani, Maher Gtari, Louis S. Tisa
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	actinorhizal symbiosis microbiome endophyte symbiont plant-growth-promoting bacteria Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f48da7a3df8a46a2986b5ead919fbb8e
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spelling	oai:doaj.org-article:f48da7a3df8a46a2986b5ead919fbb8e2021-12-02T10:01:13ZAlone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules1664-302X10.3389/fmicb.2021.749760https://doaj.org/article/f48da7a3df8a46a2986b5ead919fbb8e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.749760/fullhttps://doaj.org/toc/1664-302XActinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.Faten Ghodhbane-GtariFaten Ghodhbane-GtariFaten Ghodhbane-GtariTimothy D’AngeloAbdellatif GueddouSabrine GhazouaniMaher GtariMaher GtariLouis S. TisaFrontiers Media S.A.articleactinorhizal symbiosismicrobiomeendophytesymbiontplant-growth-promoting bacteriaMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinorhizal symbiosis microbiome endophyte symbiont plant-growth-promoting bacteria Microbiology QR1-502
spellingShingle	actinorhizal symbiosis microbiome endophyte symbiont plant-growth-promoting bacteria Microbiology QR1-502 Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Timothy D’Angelo Abdellatif Gueddou Sabrine Ghazouani Maher Gtari Maher Gtari Louis S. Tisa Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
description	Actinorhizal plants host mutualistic symbionts of the nitrogen-fixing actinobacterial genus Frankia within nodule structures formed on their roots. Several plant-growth-promoting bacteria have also been isolated from actinorhizal root nodules, but little is known about them. We were interested investigating the in planta microbial community composition of actinorhizal root nodules using culture-independent techniques. To address this knowledge gap, 16S rRNA gene amplicon and shotgun metagenomic sequencing was performed on DNA from the nodules of Casuarina glauca. DNA was extracted from C. glauca nodules collected in three different sampling sites in Tunisia, along a gradient of aridity ranging from humid to arid. Sequencing libraries were prepared using Illumina NextEra technology and the Illumina HiSeq 2500 platform. Genome bins extracted from the metagenome were taxonomically and functionally profiled. Community structure based off preliminary 16S rRNA gene amplicon data was analyzed via the QIIME pipeline. Reconstructed genomes were comprised of members of Frankia, Micromonospora, Bacillus, Paenibacillus, Phyllobacterium, and Afipia. Frankia dominated the nodule community at the humid sampling site, while the absolute and relative prevalence of Frankia decreased at the semi-arid and arid sampling locations. Actinorhizal plants harbor similar non-Frankia plant-growth-promoting-bacteria as legumes and other plants. The data suggests that the prevalence of Frankia in the nodule community is influenced by environmental factors, with being less abundant under more arid environments.
format	article
author	Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Timothy D’Angelo Abdellatif Gueddou Sabrine Ghazouani Maher Gtari Maher Gtari Louis S. Tisa
author_facet	Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Faten Ghodhbane-Gtari Timothy D’Angelo Abdellatif Gueddou Sabrine Ghazouani Maher Gtari Maher Gtari Louis S. Tisa
author_sort	Faten Ghodhbane-Gtari
title	Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
title_short	Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
title_full	Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
title_fullStr	Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
title_full_unstemmed	Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules
title_sort	alone yet not alone: frankia lives under the same roof with other bacteria in actinorhizal nodules
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f48da7a3df8a46a2986b5ead919fbb8e
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Alone Yet Not Alone: Frankia Lives Under the Same Roof With Other Bacteria in Actinorhizal Nodules

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