Superovulation Does Not Alter Calcium Oscillations Following Fertilization

Superovulation is a common approach to maximize the number of eggs available for either clinical assisted reproductive technologies or experimental animal studies. This procedure provides supraphysiological amounts of gonadotropins to promote continued growth and maturation of ovarian follicles that...

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Autores principales:	Virginia Savy, Paula Stein, Min Shi, Carmen J. Williams
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	superovulation oocyte mouse calcium oscillations egg activation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f48dc4fad44143398b076c1ae1356685
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spelling	oai:doaj.org-article:f48dc4fad44143398b076c1ae13566852021-11-04T05:18:18ZSuperovulation Does Not Alter Calcium Oscillations Following Fertilization2296-634X10.3389/fcell.2021.762057https://doaj.org/article/f48dc4fad44143398b076c1ae13566852021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.762057/fullhttps://doaj.org/toc/2296-634XSuperovulation is a common approach to maximize the number of eggs available for either clinical assisted reproductive technologies or experimental animal studies. This procedure provides supraphysiological amounts of gonadotropins to promote continued growth and maturation of ovarian follicles that otherwise would undergo atresia. There is evidence in mice, cows, sheep, and humans that superovulation has a detrimental impact on the quality of the resulting ovulated eggs or embryos. Here we tested the hypothesis that eggs derived from superovulation have a reduced capacity to support calcium oscillations, which are a critical factor in the success of embryo development. Eggs were obtained from mice that were either naturally cycling or underwent a standard superovulation protocol. The eggs were either parthenogenetically activated using strontium or fertilized in vitro while undergoing monitoring of calcium oscillatory patterns. Following parthenogenetic activation, superovulated eggs had a slightly delayed onset and longer duration of the first calcium transient, but no differences in oscillation persistence, frequency, or total calcium signal. However, in vitro fertilized superovulated eggs had no differences in any of these measures of calcium oscillatory behavior relative to spontaneously ovulated eggs. These findings indicate that although subtle differences in calcium signaling can be detected following parthenogenetic activation, superovulation does not disrupt physiological calcium signaling at fertilization, supporting the use of this method for both clinical and experimental purposes.Virginia SavyPaula SteinMin ShiCarmen J. WilliamsFrontiers Media S.A.articlesuperovulationoocytemousecalcium oscillationsegg activationBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	superovulation oocyte mouse calcium oscillations egg activation Biology (General) QH301-705.5
spellingShingle	superovulation oocyte mouse calcium oscillations egg activation Biology (General) QH301-705.5 Virginia Savy Paula Stein Min Shi Carmen J. Williams Superovulation Does Not Alter Calcium Oscillations Following Fertilization
description	Superovulation is a common approach to maximize the number of eggs available for either clinical assisted reproductive technologies or experimental animal studies. This procedure provides supraphysiological amounts of gonadotropins to promote continued growth and maturation of ovarian follicles that otherwise would undergo atresia. There is evidence in mice, cows, sheep, and humans that superovulation has a detrimental impact on the quality of the resulting ovulated eggs or embryos. Here we tested the hypothesis that eggs derived from superovulation have a reduced capacity to support calcium oscillations, which are a critical factor in the success of embryo development. Eggs were obtained from mice that were either naturally cycling or underwent a standard superovulation protocol. The eggs were either parthenogenetically activated using strontium or fertilized in vitro while undergoing monitoring of calcium oscillatory patterns. Following parthenogenetic activation, superovulated eggs had a slightly delayed onset and longer duration of the first calcium transient, but no differences in oscillation persistence, frequency, or total calcium signal. However, in vitro fertilized superovulated eggs had no differences in any of these measures of calcium oscillatory behavior relative to spontaneously ovulated eggs. These findings indicate that although subtle differences in calcium signaling can be detected following parthenogenetic activation, superovulation does not disrupt physiological calcium signaling at fertilization, supporting the use of this method for both clinical and experimental purposes.
format	article
author	Virginia Savy Paula Stein Min Shi Carmen J. Williams
author_facet	Virginia Savy Paula Stein Min Shi Carmen J. Williams
author_sort	Virginia Savy
title	Superovulation Does Not Alter Calcium Oscillations Following Fertilization
title_short	Superovulation Does Not Alter Calcium Oscillations Following Fertilization
title_full	Superovulation Does Not Alter Calcium Oscillations Following Fertilization
title_fullStr	Superovulation Does Not Alter Calcium Oscillations Following Fertilization
title_full_unstemmed	Superovulation Does Not Alter Calcium Oscillations Following Fertilization
title_sort	superovulation does not alter calcium oscillations following fertilization
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f48dc4fad44143398b076c1ae1356685
work_keys_str_mv	AT virginiasavy superovulationdoesnotaltercalciumoscillationsfollowingfertilization AT paulastein superovulationdoesnotaltercalciumoscillationsfollowingfertilization AT minshi superovulationdoesnotaltercalciumoscillationsfollowingfertilization AT carmenjwilliams superovulationdoesnotaltercalciumoscillationsfollowingfertilization
_version_	1718445248257982464

Superovulation Does Not Alter Calcium Oscillations Following Fertilization

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