Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil

Abstract Optochin susceptibility testing is a major assay used for presumptive identification of Streptococcus pneumoniae. Still, atypical optochin-resistant (Optr) pneumococci have been reported and this phenotype has been attributed to nucleotide substitutions in the genes coding for the F0F1ATPas...

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Autores principales:	Aline R. V. Souza, Sandrine E. C. M. de Pina, Natália S. Costa, Felipe P. G. Neves, Vânia L. C. Merquior, José Mauro Peralta, Tatiana C. A. Pinto, Lúcia M. Teixeira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f49136c3acaa44c180b4936f4e0a0e5b
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spelling	oai:doaj.org-article:f49136c3acaa44c180b4936f4e0a0e5b2021-12-02T15:51:15ZDescription of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil10.1038/s41598-021-87071-82045-2322https://doaj.org/article/f49136c3acaa44c180b4936f4e0a0e5b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87071-8https://doaj.org/toc/2045-2322Abstract Optochin susceptibility testing is a major assay used for presumptive identification of Streptococcus pneumoniae. Still, atypical optochin-resistant (Optr) pneumococci have been reported and this phenotype has been attributed to nucleotide substitutions in the genes coding for the F0F1ATPase. While substitutions in the atpC gene (c-subunit of ATPase) are more common and better characterized, data on mutations in the atpA (a-subunit) are still limited. We have characterized five Optr isolates presenting alterations in the atpA (Trp206Cys in four isolates and Trp206Ser in one isolate), constituting the first report of such mutations in Brazil. Most of the Optr isolates consisted of heterogeneous populations. Except for Opt MICs and the nucleotide changes in the atpA gene, Optr and Opts subpopulations originating from the same culture had identical characteristics. In addition, we compared phenotypic and genetic characteristics of these atpA mutants with those of atpC mutants previously identified in Brazil. No structural alterations were detected among predicted proteins, regardless of mutations in the coding gene, suggesting that, despite the occurrence of mutations, protein structures tend to be highly conserved, ensuring their functionalities. Phylogenetic analysis revealed that atypical Optr strains are true pneumococci and Opt resistance does not represent any apparent selective advantage for clinical isolates.Aline R. V. SouzaSandrine E. C. M. de PinaNatália S. CostaFelipe P. G. NevesVânia L. C. MerquiorJosé Mauro PeraltaTatiana C. A. PintoLúcia M. TeixeiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aline R. V. Souza Sandrine E. C. M. de Pina Natália S. Costa Felipe P. G. Neves Vânia L. C. Merquior José Mauro Peralta Tatiana C. A. Pinto Lúcia M. Teixeira Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
description	Abstract Optochin susceptibility testing is a major assay used for presumptive identification of Streptococcus pneumoniae. Still, atypical optochin-resistant (Optr) pneumococci have been reported and this phenotype has been attributed to nucleotide substitutions in the genes coding for the F0F1ATPase. While substitutions in the atpC gene (c-subunit of ATPase) are more common and better characterized, data on mutations in the atpA (a-subunit) are still limited. We have characterized five Optr isolates presenting alterations in the atpA (Trp206Cys in four isolates and Trp206Ser in one isolate), constituting the first report of such mutations in Brazil. Most of the Optr isolates consisted of heterogeneous populations. Except for Opt MICs and the nucleotide changes in the atpA gene, Optr and Opts subpopulations originating from the same culture had identical characteristics. In addition, we compared phenotypic and genetic characteristics of these atpA mutants with those of atpC mutants previously identified in Brazil. No structural alterations were detected among predicted proteins, regardless of mutations in the coding gene, suggesting that, despite the occurrence of mutations, protein structures tend to be highly conserved, ensuring their functionalities. Phylogenetic analysis revealed that atypical Optr strains are true pneumococci and Opt resistance does not represent any apparent selective advantage for clinical isolates.
format	article
author	Aline R. V. Souza Sandrine E. C. M. de Pina Natália S. Costa Felipe P. G. Neves Vânia L. C. Merquior José Mauro Peralta Tatiana C. A. Pinto Lúcia M. Teixeira
author_facet	Aline R. V. Souza Sandrine E. C. M. de Pina Natália S. Costa Felipe P. G. Neves Vânia L. C. Merquior José Mauro Peralta Tatiana C. A. Pinto Lúcia M. Teixeira
author_sort	Aline R. V. Souza
title	Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
title_short	Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
title_full	Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
title_fullStr	Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
title_full_unstemmed	Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil
title_sort	description of optochin-resistant streptococcus pneumoniae due to an uncommon mutation in the atpa gene and comparison with previously identified atpc mutants from brazil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f49136c3acaa44c180b4936f4e0a0e5b
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Description of optochin-resistant Streptococcus pneumoniae due to an uncommon mutation in the atpA gene and comparison with previously identified atpC mutants from Brazil

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