Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

ABSTRACT Tacaribe virus (TCRV) is a mammalian arenavirus that was first isolated from artibeus bats in the 1950s. Subsequent experimental infection of Jamaican fruit bats (Artibeus jamaicensis) caused a disease similar to that of naturally infected bats. Although substantial attention has focused on...

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Autores principales:	Diana L. Gerrard, Ann Hawkinson, Tyler Sherman, Cassandra M. Modahl, Gretchen Hume, Corey L. Campbell, Tony Schountz, Seth Frietze
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	arenavirus bats transcriptome virus-host interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f4921e1e7cfd434d94e67e3f7110bab4
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spelling	oai:doaj.org-article:f4921e1e7cfd434d94e67e3f7110bab42021-11-15T15:22:05ZTranscriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats10.1128/mSphere.00245-172379-5042https://doaj.org/article/f4921e1e7cfd434d94e67e3f7110bab42017-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00245-17https://doaj.org/toc/2379-5042ABSTRACT Tacaribe virus (TCRV) is a mammalian arenavirus that was first isolated from artibeus bats in the 1950s. Subsequent experimental infection of Jamaican fruit bats (Artibeus jamaicensis) caused a disease similar to that of naturally infected bats. Although substantial attention has focused on bats as reservoir hosts of viruses that cause human disease, little is known about the interactions between bats and their pathogens. We performed a transcriptome-wide study to illuminate the response of Jamaican fruit bats experimentally infected with TCRV. Differential gene expression analysis of multiple tissues revealed global and organ-specific responses associated with innate antiviral responses, including interferon alpha/beta and Toll-like receptor signaling, activation of complement cascades, and cytokine signaling, among others. Genes encoding proteins involved in adaptive immune responses, such as gamma interferon signaling and costimulation of T cells by the CD28 family, were also altered in response to TCRV infection. Immunoglobulin gene expression was also elevated in the spleens of infected bats, including IgG, IgA, and IgE isotypes. These results indicate an active innate and adaptive immune response to TCRV infection occurred but did not prevent fatal disease. This de novo assembly provides a high-throughput data set of the Jamaican fruit bat and its host response to TCRV infection, which remains a valuable tool to understand the molecular signatures involved in antiviral responses in bats. IMPORTANCE As reservoir hosts of viruses associated with human disease, little is known about the interactions between bats and viruses. Using Jamaican fruit bats infected with Tacaribe virus (TCRV) as a model, we characterized the gene expression responses to infection in different tissues and identified pathways involved with the response to infection. This report is the most detailed gene discovery work in the species to date and the first to describe immune gene expression responses in bats during a pathogenic viral infection.Diana L. GerrardAnn HawkinsonTyler ShermanCassandra M. ModahlGretchen HumeCorey L. CampbellTony SchountzSeth FrietzeAmerican Society for Microbiologyarticlearenavirusbatstranscriptomevirus-host interactionsMicrobiologyQR1-502ENmSphere, Vol 2, Iss 5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	arenavirus bats transcriptome virus-host interactions Microbiology QR1-502
spellingShingle	arenavirus bats transcriptome virus-host interactions Microbiology QR1-502 Diana L. Gerrard Ann Hawkinson Tyler Sherman Cassandra M. Modahl Gretchen Hume Corey L. Campbell Tony Schountz Seth Frietze Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
description	ABSTRACT Tacaribe virus (TCRV) is a mammalian arenavirus that was first isolated from artibeus bats in the 1950s. Subsequent experimental infection of Jamaican fruit bats (Artibeus jamaicensis) caused a disease similar to that of naturally infected bats. Although substantial attention has focused on bats as reservoir hosts of viruses that cause human disease, little is known about the interactions between bats and their pathogens. We performed a transcriptome-wide study to illuminate the response of Jamaican fruit bats experimentally infected with TCRV. Differential gene expression analysis of multiple tissues revealed global and organ-specific responses associated with innate antiviral responses, including interferon alpha/beta and Toll-like receptor signaling, activation of complement cascades, and cytokine signaling, among others. Genes encoding proteins involved in adaptive immune responses, such as gamma interferon signaling and costimulation of T cells by the CD28 family, were also altered in response to TCRV infection. Immunoglobulin gene expression was also elevated in the spleens of infected bats, including IgG, IgA, and IgE isotypes. These results indicate an active innate and adaptive immune response to TCRV infection occurred but did not prevent fatal disease. This de novo assembly provides a high-throughput data set of the Jamaican fruit bat and its host response to TCRV infection, which remains a valuable tool to understand the molecular signatures involved in antiviral responses in bats. IMPORTANCE As reservoir hosts of viruses associated with human disease, little is known about the interactions between bats and viruses. Using Jamaican fruit bats infected with Tacaribe virus (TCRV) as a model, we characterized the gene expression responses to infection in different tissues and identified pathways involved with the response to infection. This report is the most detailed gene discovery work in the species to date and the first to describe immune gene expression responses in bats during a pathogenic viral infection.
format	article
author	Diana L. Gerrard Ann Hawkinson Tyler Sherman Cassandra M. Modahl Gretchen Hume Corey L. Campbell Tony Schountz Seth Frietze
author_facet	Diana L. Gerrard Ann Hawkinson Tyler Sherman Cassandra M. Modahl Gretchen Hume Corey L. Campbell Tony Schountz Seth Frietze
author_sort	Diana L. Gerrard
title	Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
title_short	Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
title_full	Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
title_fullStr	Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
title_full_unstemmed	Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats
title_sort	transcriptomic signatures of tacaribe virus-infected jamaican fruit bats
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/f4921e1e7cfd434d94e67e3f7110bab4
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Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats

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