Protective activity of specific transfer factor against Salmonella typhimurium infection

Specific transfer factor (TF) extracted from spleens of sensitized and non-sensitized guinea pigs to study the efficacy of transfer of cellular immunity specific for salmonella . Two groups each of five guinea pigs were used for in vivo TF preparation. The First group was inoculated with 1 ml of ar...

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Autores principales:	A. A. Yousif, K. A. Khalifa, S. A.G. Al-Samarrae
Formato:	article
Lenguaje:	EN
Publicado:	University of Baghdad, College of Veterinary Medicine 2021
Materias:	Salmonella typhimurium infection Protective activity Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/f4990b97dcc3498984ebe334f3daf130
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spelling	oai:doaj.org-article:f4990b97dcc3498984ebe334f3daf1302021-11-06T11:33:51ZProtective activity of specific transfer factor against Salmonella typhimurium infection10.30539/ijvm.v25i1.11561609-56932410-7409https://doaj.org/article/f4990b97dcc3498984ebe334f3daf1302021-11-01T00:00:00Zhttps://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1156https://doaj.org/toc/1609-5693https://doaj.org/toc/2410-7409 Specific transfer factor (TF) extracted from spleens of sensitized and non-sensitized guinea pigs to study the efficacy of transfer of cellular immunity specific for salmonella . Two groups each of five guinea pigs were used for in vivo TF preparation. The First group was inoculated with 1 ml of aromatic dependent Salmonella typhimurium SL 1479 vaccine at a dose of 10' cfu/ml intramuscularly twice at two weeks intervals. The second group was injected with trypticase soy broth similarly . These two groups used as a donor for TF, and TFn respectively. Twenty one recipient guinea pigs were divided into three groups, the first group was TF at a dose of 1 ml equivalent to 5X10 cell intramuscularly three times/2 days intervals, Similarly the second group was given TF, where as the third group was given PBS- Cell mediated immunity in recipient animal was evaluated by delayed type hypersensitivity - skin test, Macrophage migration inhibition test (MIF) and then challenged with virulent Salmonella typhimurium. The TFt recipient group induced skin test and showed migration indices less than 0.8 and overcome the challenge organism. Contrary to TFn & PBS recipient groups which did not show any response for skin test and given migration indices more than 0.8 and did not show resistance for virulent Salmonella typhimurium . A. A. YousifK. A. KhalifaS. A.G. Al-SamarraeUniversity of Baghdad, College of Veterinary MedicinearticleSalmonellatyphimurium infectionProtective activityVeterinary medicineSF600-1100ENThe Iraqi Journal of Veterinary Medicine, Vol 25, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella typhimurium infection Protective activity Veterinary medicine SF600-1100
spellingShingle	Salmonella typhimurium infection Protective activity Veterinary medicine SF600-1100 A. A. Yousif K. A. Khalifa S. A.G. Al-Samarrae Protective activity of specific transfer factor against Salmonella typhimurium infection
description	Specific transfer factor (TF) extracted from spleens of sensitized and non-sensitized guinea pigs to study the efficacy of transfer of cellular immunity specific for salmonella . Two groups each of five guinea pigs were used for in vivo TF preparation. The First group was inoculated with 1 ml of aromatic dependent Salmonella typhimurium SL 1479 vaccine at a dose of 10' cfu/ml intramuscularly twice at two weeks intervals. The second group was injected with trypticase soy broth similarly . These two groups used as a donor for TF, and TFn respectively. Twenty one recipient guinea pigs were divided into three groups, the first group was TF at a dose of 1 ml equivalent to 5X10 cell intramuscularly three times/2 days intervals, Similarly the second group was given TF, where as the third group was given PBS- Cell mediated immunity in recipient animal was evaluated by delayed type hypersensitivity - skin test, Macrophage migration inhibition test (MIF) and then challenged with virulent Salmonella typhimurium. The TFt recipient group induced skin test and showed migration indices less than 0.8 and overcome the challenge organism. Contrary to TFn & PBS recipient groups which did not show any response for skin test and given migration indices more than 0.8 and did not show resistance for virulent Salmonella typhimurium .
format	article
author	A. A. Yousif K. A. Khalifa S. A.G. Al-Samarrae
author_facet	A. A. Yousif K. A. Khalifa S. A.G. Al-Samarrae
author_sort	A. A. Yousif
title	Protective activity of specific transfer factor against Salmonella typhimurium infection
title_short	Protective activity of specific transfer factor against Salmonella typhimurium infection
title_full	Protective activity of specific transfer factor against Salmonella typhimurium infection
title_fullStr	Protective activity of specific transfer factor against Salmonella typhimurium infection
title_full_unstemmed	Protective activity of specific transfer factor against Salmonella typhimurium infection
title_sort	protective activity of specific transfer factor against salmonella typhimurium infection
publisher	University of Baghdad, College of Veterinary Medicine
publishDate	2021
url	https://doaj.org/article/f4990b97dcc3498984ebe334f3daf130
work_keys_str_mv	AT aayousif protectiveactivityofspecifictransferfactoragainstsalmonellatyphimuriuminfection AT kakhalifa protectiveactivityofspecifictransferfactoragainstsalmonellatyphimuriuminfection AT sagalsamarrae protectiveactivityofspecifictransferfactoragainstsalmonellatyphimuriuminfection
_version_	1718443707261255680

Protective activity of specific transfer factor against Salmonella typhimurium infection

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