Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)

Abstract Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis o...

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Autores principales:	Ping Liu, Zi-Xiang Yang, Xiao-Ming Chen, Hang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4a924b54a5f4771a036fd32fbda026a
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spelling	oai:doaj.org-article:f4a924b54a5f4771a036fd32fbda026a2021-12-02T15:05:09ZFull-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)10.1038/s41598-017-06806-82045-2322https://doaj.org/article/f4a924b54a5f4771a036fd32fbda026a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06806-8https://doaj.org/toc/2045-2322Abstract Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis of Rhus chinensis. S. chinensis has a complex life cycle, with a switch of hosts between R. chinensis and certain mosses, and a switch of sexual and asexual reproduction (cyclical parthenogenesis). We have cloned a full-length cDNA of the β-tubulin gene from S. chinensis, using qPCR and RACE. This cDNA has 1606 base pairs with a 251 bp 5′-untranslated region (5′-UTR) and a 15 bp 3′-untranslated region (3′-UTR). The gene encodes a protein with 376 amino acids residues. The expression levels of the β-tubulin gene in S. chinensis were investigated among fundatrigeniae and overwintering larvae rearing under either natural conditions, or at 7.5 °C and 18 °C. No significant differences (P > 0.01) in gene expression levels were found in insects under these conditions. It is indicates that the β-tubulin gene is highly conserved and then it may be used as a reference for further research in gene expression and reproduction determination in this important aphid.Ping LiuZi-Xiang YangXiao-Ming ChenHang ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping Liu Zi-Xiang Yang Xiao-Ming Chen Hang Chen Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
description	Abstract Some insect galls are formed on sumac plants by certain aphid species and have been used for medicinal and chemical purposes as they are rich in tannins. The most prominent species among gall aphids in China is Schlechtendalia chinensis, which formed horn-shaped galls on the winged rachis of Rhus chinensis. S. chinensis has a complex life cycle, with a switch of hosts between R. chinensis and certain mosses, and a switch of sexual and asexual reproduction (cyclical parthenogenesis). We have cloned a full-length cDNA of the β-tubulin gene from S. chinensis, using qPCR and RACE. This cDNA has 1606 base pairs with a 251 bp 5′-untranslated region (5′-UTR) and a 15 bp 3′-untranslated region (3′-UTR). The gene encodes a protein with 376 amino acids residues. The expression levels of the β-tubulin gene in S. chinensis were investigated among fundatrigeniae and overwintering larvae rearing under either natural conditions, or at 7.5 °C and 18 °C. No significant differences (P > 0.01) in gene expression levels were found in insects under these conditions. It is indicates that the β-tubulin gene is highly conserved and then it may be used as a reference for further research in gene expression and reproduction determination in this important aphid.
format	article
author	Ping Liu Zi-Xiang Yang Xiao-Ming Chen Hang Chen
author_facet	Ping Liu Zi-Xiang Yang Xiao-Ming Chen Hang Chen
author_sort	Ping Liu
title	Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
title_short	Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
title_full	Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
title_fullStr	Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
title_full_unstemmed	Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)
title_sort	full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the chinese gall aphid (schlechtendalia chinensis)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f4a924b54a5f4771a036fd32fbda026a
work_keys_str_mv	AT pingliu fulllengthcloningsequenceanalysisandexpressiondetectionofthebtubulingenefromthechinesegallaphidschlechtendaliachinensis AT zixiangyang fulllengthcloningsequenceanalysisandexpressiondetectionofthebtubulingenefromthechinesegallaphidschlechtendaliachinensis AT xiaomingchen fulllengthcloningsequenceanalysisandexpressiondetectionofthebtubulingenefromthechinesegallaphidschlechtendaliachinensis AT hangchen fulllengthcloningsequenceanalysisandexpressiondetectionofthebtubulingenefromthechinesegallaphidschlechtendaliachinensis
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Full-length cloning, sequence analysis and expression detection of the β-tubulin gene from the Chinese gall aphid (Schlechtendalia chinensis)

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