DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase

Abstract DNA polymerase (pol) processivity, i.e., the bases a polymerase extends before falling off the DNA, and activity are important for copying difficult DNA sequences, including simple repeats. Y-family pols would be appealing for copying difficult DNA and incorporating non-natural dNTPs, due t...

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Autores principales:	Jing Wu, Alexandra de Paz, Bradley M. Zamft, Adam H. Marblestone, Edward S. Boyden, Konrad P. Kording, Keith E. J. Tyo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f4b1ec7d932b45bca5803a0278a5f0b6
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spelling	oai:doaj.org-article:f4b1ec7d932b45bca5803a0278a5f0b62021-12-02T11:40:32ZDNA binding strength increases the processivity and activity of a Y-Family DNA polymerase10.1038/s41598-017-02578-32045-2322https://doaj.org/article/f4b1ec7d932b45bca5803a0278a5f0b62017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02578-3https://doaj.org/toc/2045-2322Abstract DNA polymerase (pol) processivity, i.e., the bases a polymerase extends before falling off the DNA, and activity are important for copying difficult DNA sequences, including simple repeats. Y-family pols would be appealing for copying difficult DNA and incorporating non-natural dNTPs, due to their low fidelity and loose active site, but are limited by poor processivity and activity. In this study, the binding between Dbh and DNA was investigated to better understand how to rationally design enhanced processivity in a Y-family pol. Guided by structural simulation, a fused pol Sdbh with non-specific dsDNA binding protein Sso7d in the N-terminus was designed. This modification increased in vitro processivity 4-fold as compared to the wild-type Dbh. Additionally, bioinformatics was used to identify amino acid mutations that would increase stabilization of Dbh bound to DNA. The variant SdbhM76I further improved the processivity of Dbh by 10 fold. The variant SdbhKSKIP241–245RVRKS showed higher activity than Dbh on the incorporation of dCTP (correct) and dATP (incorrect) opposite the G (normal) or 8-oxoG(damaged) template base. These results demonstrate the capability to rationally design increases in pol processivity and catalytic efficiency through computational DNA binding predictions and the addition of non-specific DNA binding domains.Jing WuAlexandra de PazBradley M. ZamftAdam H. MarblestoneEdward S. BoydenKonrad P. KordingKeith E. J. TyoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Wu Alexandra de Paz Bradley M. Zamft Adam H. Marblestone Edward S. Boyden Konrad P. Kording Keith E. J. Tyo DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
description	Abstract DNA polymerase (pol) processivity, i.e., the bases a polymerase extends before falling off the DNA, and activity are important for copying difficult DNA sequences, including simple repeats. Y-family pols would be appealing for copying difficult DNA and incorporating non-natural dNTPs, due to their low fidelity and loose active site, but are limited by poor processivity and activity. In this study, the binding between Dbh and DNA was investigated to better understand how to rationally design enhanced processivity in a Y-family pol. Guided by structural simulation, a fused pol Sdbh with non-specific dsDNA binding protein Sso7d in the N-terminus was designed. This modification increased in vitro processivity 4-fold as compared to the wild-type Dbh. Additionally, bioinformatics was used to identify amino acid mutations that would increase stabilization of Dbh bound to DNA. The variant SdbhM76I further improved the processivity of Dbh by 10 fold. The variant SdbhKSKIP241–245RVRKS showed higher activity than Dbh on the incorporation of dCTP (correct) and dATP (incorrect) opposite the G (normal) or 8-oxoG(damaged) template base. These results demonstrate the capability to rationally design increases in pol processivity and catalytic efficiency through computational DNA binding predictions and the addition of non-specific DNA binding domains.
format	article
author	Jing Wu Alexandra de Paz Bradley M. Zamft Adam H. Marblestone Edward S. Boyden Konrad P. Kording Keith E. J. Tyo
author_facet	Jing Wu Alexandra de Paz Bradley M. Zamft Adam H. Marblestone Edward S. Boyden Konrad P. Kording Keith E. J. Tyo
author_sort	Jing Wu
title	DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
title_short	DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
title_full	DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
title_fullStr	DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
title_full_unstemmed	DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
title_sort	dna binding strength increases the processivity and activity of a y-family dna polymerase
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f4b1ec7d932b45bca5803a0278a5f0b6
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DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase

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